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11.
刘小丽’.薛晓鸥’.唐炳华.牛建昭 《勤云标准版测试》2011,31(1):25-29
〔摘要〕目的探讨大豆葺元对子宫内膜癌细胞增殖的影响及其对雌激素受体报告基因表达的影响、方法体
外培养子宫内膜癌细胞子宫内膜癌雌、孕激素受体阴性细胞系(HEC 1B),采用四甲基偶氮哩盐法(M1"P)检测不同
浓度大豆葺元对子宫内膜癌细胞增殖的影响,同时检测大豆葺元对雌激素应答原件(ERE)调控的雌激素受体。
(ERa)和雌激素受体(3 ( ER(3)的荧光素酶报告基因表达的影响〔结果大豆葺元对HEC-1B细胞体外增殖有明显
抑制作用,差异有统计学意义(P<0.01),其抑制作用有明显量效和时效性特征〔在浓度为20-80x 1 O}mol/L的大豆葺
元作用卜,报告基因1u。的表达较正常对照组明显提高,差异有统计学意义(P<0.05 ),在80x 10-`mol/L时到达最高
值;大豆葺元通过ER日介导的报告基因表达水平的升高程度强于ERa,差异有统计学意义(P<0.01)〔结论大豆葺
元可通过调节子宫内膜癌细胞ER。和ER日介导的报告基因的表达,调整ERa/ER日比例,从而抑制子宫内膜癌细
胞的增殖、 相似文献
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用PCR(聚合酶链式反应)法扩增来源于几个硬粒小麦单粒萌发种子DNA提取液中的LMW(低分子量)谷蛋白基因序列.PCR反应的电泳分析表明,品质优劣不同的硬拉小麦品种具有不同的扩增产品.这种建立在PCR基础上的方法,比标准方法筛选优异品质基因型更有效、更安全. 相似文献
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Background: Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results. The purpose of this study was to identify the most stable housekeeping genes in porcine articular cartilage subjected to a mechanical injury from a panel of 10 candidate genes. Results: Ten candidate housekeeping genes were evaluated in three different treatment groups of mechanically impacted porcine articular cartilage. The genes evaluated were: beta actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hydroxymethylbilane synthase, hypoxanthine phosphoribosyl transferase, peptidylprolyl isomerase A (cyclophilin A), ribosomal protein L4, succinate dehydrogenase flavoprotein subunit A, TATA box binding protein, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein-zeta polypeptide. The stability of the genes was measured using geNorm, BestKeeper, and NormFinder software. The four most stable genes measured via geNorm were (most to least stable) succinate dehydrogenase flavoprotein, subunit A, peptidylprolyl isomerase A, glyceraldehyde-3-phosphate dehydrogenase, beta actin; the four most stable genes measured via BestKeeper were glyceraldehyde-3-phosphate dehydrogenase, peptidylprolyl isomerase A, beta actin, succinate dehydrogenase flavoprotein, subunit A; and the four most stable genes measured via NormFinder were peptidylprolyl isomerase A, succinate dehydrogenase flavoprotein, subunit A, glyceraldehyde-3-phosphate dehydrogenase, beta actin. Conclusions: BestKeeper, geNorm, and NormFinder all generated similar results for the most stable genes in porcine articular cartilage. The use of these appropriate reference genes will facilitate accurate gene expression studies of porcine articular cartilage and suggest appropriate housekeeping genes for articular cartilage studies in other species. 相似文献
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Seventeen mangrove species of eight families at seven riverine and fringe habitats in Goa West India were surveyed for Arbuscular Mycorrhizal (AM) fungal diversity. Sixteen species were found to be mycorrhizal and one species showed no AM fungal colonization. AM root colonization was recorded at all seven sites and ranged from 6%-77%. Maximum root colonization was recorded in Excoecaria agallocha (77%) and minimum colonization in Avicennia marina (6%). Paris-type colonization was predominant at all sites. Auxiliary cells were recorded in roots of Acanthus ilicifolius, Ceriops tagal and Sonneretia alba. AM fungal root colonization and spore density varied by plant species and site. Site average spore density ranged from 1.84 spores·g-1 to 0.54 spores·g-1 of soil. In total, 28 AM fungal species of five genera, viz. Glomus, Acaulospora, Scutellospora, Gigaspora and Entrophospora, were recovered. Glomus was the dominant genus, three species of which were sporocarpic forms. Maximum site species richness (SR) ranged from 16 to 5. Species richness was maximum in A. ilicifolius where seven species of three genera were recovered. Based on relative abundance (RA) and isolation frequency (IF), two common species, viz. G. intraradices and A. laevis, were recovered from all seven sites. 相似文献
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张加力’陈茹’于冰’温伟 《中国兽医杂志》2014,(4):50-51
<正>仔猪黄痢是新生仔猪的急性、高致死性传病。主要发生于1周龄内的新生仔猪,以13日龄发病最多,随日龄的增长发病减少,1周龄以上很少发病。初产母猪所产仔猪发病最为严重,而经产母猪所产仔猪发病相对较轻。猪场卫生条件不好,新生仔猪初乳吃得不够或母猪乳汁不足以及产房温度 相似文献
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〔摘要〕目的明确小儿手足ii病辨证论治治法、方药、内服、外用、中药注射液,为进一步制定科学、客观的小儿
辨证论治方案提供参考、方法根据文献研究结果,通过德尔菲法制定问卷,利用SPSS软件对数据进行积极度、集中
程度、协调程度、权威程度的统计与评价〔结果专家对7个证候对应的治法、方药协调程度一致,对2个中药注射
液、4个}}服中成药、3个外用中成药协调程度一致、结论专家问卷调查是分析小儿辨证论治方案的有效方法、 相似文献
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〔摘要〕目的研究盐酸青藤碱的透皮吸收及离子导入方法对透皮吸收的影响、方法采用家兔离体皮肤及
Franz扩散池进行体外透皮试验,并以电子治疗仪作为外加电场进行离子导入,观测盐酸青藤碱注射液的透皮渗透
率,并比较外加电场对其透皮渗透量的影响、结果盐酸青藤碱的透皮量随着时间的延长而增加,符合零级动力学
方程( R==0.952 1)、外加电场可增加其透皮吸收量,其中以电场强度20 mA、通电时间15 min的透皮量较为I}想、
结论盐酸青藤碱注射液可透皮吸收,电场导入对其透皮吸收有一定的促进作用、木研究为临床中以盐酸青藤碱注
射液进行离子导入的治疗方法提供了实验依据、 相似文献
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段春艳’.陈元.李定祥.邓奕辉 《勤云标准版测试》2011,31(1):36-39
〔摘要〕目的观察滋阴活血解毒饮片及超微方对大鼠局灶性脑缺血后白细胞介素一1日(IL-1日)、白细胞介素-
8(IL-8)、肿瘤坏死因子一。(TNF-a)含量的影响、方法将25只大鼠随机分为假手术组、模型组、滋阴活血解毒方饮
片组(以卜简称饮片组)、滋阴活血解毒方超微组(以卜简称超微组)、阿加曲班组,每组5只、大脑中动脉线栓法复
制局灶性脑缺血模型,进行神经功能评分〔造模成功后7d,用TTC染色法测定脑梗死范围,用Motic Med6.0数码
图像分析系统分析脑梗死面积〔采用ELISA法测定大鼠血清IL-1日,IL-8 ,TNF-。的含量〔结果饮片组、超微组及
阿加曲班组大鼠脑梗死体积比均明显低于模型组,差异具有统计学意义(P<0.01) ;模型组血清IL-1日,IL-8,TNF-a
含量明显高于假手术组(P<0.01),饮片组、超微组、阿加曲班组均能明显降低大鼠血清IL-1日、IL-8 , TNF-a含量,
与模型组比较,差异均有统计学意义(P<0.01)、结论滋阴活血解毒方能减小脑梗死面积,抑制炎性因子的表达,降
低脑缺血所致的脑损伤、 相似文献
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〔摘要〕目的初步了解昆明市部分社区高血压及其合并心衰人群的中医体质类型分布状况,探讨中医体质与
高血压合并心衰发病的相关性〔方法采用标准化的9种中医体质量表对高血压合并心衰人群进行横断面现状调
查〔结果所有调查414例有效问卷中,高血压人群376例,正常对照组38例〔其中高血压人群平和质117例,单纯
偏颇体质86例,兼夹偏颇体质173例〔所有人群气虚质频次最多,且正常血压、高血压及高血压合并心衰人群中医
体质分布比较,差异均具有统计学意义(P<0.05 )、结论中医体质具有其复杂性,气虚体质在木地区老年人高血压患
者存在普遍性.阳虚质在致高血压心衰中具有决定性.疲血质及痰湿质也是致高血压合并心衰发病的重要体质、 相似文献