昆明地区高血压人群中医体质分布特点调查分析

〔摘要〕目的初步了解昆明市部分社区高血压及其合并心衰人群的中医体质类型分布状况,探讨中医体质与 高血压合并心衰发病的相关性〔方法采用标准化的9种中医体质量表对高血压合并心衰人群进行横断面现状调 查〔结果所有调查414例有效问卷中,高血压人群376例,正常对照组38例〔其中高血压人群平和质117例,单纯 偏颇体质86例,兼夹偏颇体质173例〔所有人群气虚质频次最多,且正常血压、高血压及高血压合并心衰人群中医 体质分布比较,差异均具有统计学意义(P<0.05 )、结论中医体质具有其复杂性,气虚体质在木地区老年人高血压患 者存在普遍性.阳虚质在致高血压心衰中具有决定性.疲血质及痰湿质也是致高血压合并心衰发病的重要体质、     

滋阴活血解毒方对局灶性脑缺血模型大鼠 血清中IL-1(3,IL-8,TNF-。含量的影响

〔摘要〕目的观察滋阴活血解毒饮片及超微方对大鼠局灶性脑缺血后白细胞介素一1日(IL-1日)、白细胞介素- 8(IL-8)、肿瘤坏死因子一。(TNF-a)含量的影响、方法将25只大鼠随机分为假手术组、模型组、滋阴活血解毒方饮 片组(以卜简称饮片组)、滋阴活血解毒方超微组(以卜简称超微组)、阿加曲班组,每组5只、大脑中动脉线栓法复 制局灶性脑缺血模型,进行神经功能评分〔造模成功后7d,用TTC染色法测定脑梗死范围,用Motic Med6.0数码 图像分析系统分析脑梗死面积〔采用ELISA法测定大鼠血清IL-1日,IL-8 ,TNF-。的含量〔结果饮片组、超微组及 阿加曲班组大鼠脑梗死体积比均明显低于模型组,差异具有统计学意义(P<0.01) ;模型组血清IL-1日,IL-8,TNF-a 含量明显高于假手术组(P<0.01),饮片组、超微组、阿加曲班组均能明显降低大鼠血清IL-1日、IL-8 , TNF-a含量, 与模型组比较,差异均有统计学意义(P<0.01)、结论滋阴活血解毒方能减小脑梗死面积,抑制炎性因子的表达,降 低脑缺血所致的脑损伤、     

PKP术治疗老年骨质疏松性胸腰椎 压缩性骨折的临床观察

〔摘要〕目的观察经皮椎体后凸成形术(percutaneous kyphoplasty , PKP)治疗老年骨质疏松性压缩性骨折的临 床疗效、方法对收治的符合纳入标准的38例老年骨质疏松性胸腰椎压缩性骨折患者应用PKP治疗并进行临床 疗效和影像学分析〔术后随访2个月、结果术后腰背部疼痛消失35例,疼痛明显减轻3例,无骨水泥渗漏〔术后 行影像学检查未见椎管内占位,后凸畸形明显改善、术后伤椎前柱平均高度明显提高(P<0.05 ) , Cobb角恢复至正 常水平,与术前比较差异有统计学意义(P<0.05 )、结论PKP术创伤小、安全、临床疗效好,能迅速缓解疼痛,有效恢 复伤椎高度,是治疗老年骨质疏松性胸腰椎压缩性骨折的理想治疗方法、     

Identification of stable normalization genes for quantitative real-time PCP, in porcine articular cartilage

Background: Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results. The purpose of this study was to identify the most stable housekeeping genes in porcine articular cartilage subjected to a mechanical injury from a panel of 10 candidate genes. Results: Ten candidate housekeeping genes were evaluated in three different treatment groups of mechanically impacted porcine articular cartilage. The genes evaluated were: beta actin, beta-2-microglobulin, glyceraldehyde-3-phosphate dehydrogenase, hydroxymethylbilane synthase, hypoxanthine phosphoribosyl transferase, peptidylprolyl isomerase A (cyclophilin A), ribosomal protein L4, succinate dehydrogenase flavoprotein subunit A, TATA box binding protein, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein-zeta polypeptide. The stability of the genes was measured using geNorm, BestKeeper, and NormFinder software. The four most stable genes measured via geNorm were (most to least stable) succinate dehydrogenase flavoprotein, subunit A, peptidylprolyl isomerase A, glyceraldehyde-3-phosphate dehydrogenase, beta actin; the four most stable genes measured via BestKeeper were glyceraldehyde-3-phosphate dehydrogenase, peptidylprolyl isomerase A, beta actin, succinate dehydrogenase flavoprotein, subunit A; and the four most stable genes measured via NormFinder were peptidylprolyl isomerase A, succinate dehydrogenase flavoprotein, subunit A, glyceraldehyde-3-phosphate dehydrogenase, beta actin. Conclusions: BestKeeper, geNorm, and NormFinder all generated similar results for the most stable genes in porcine articular cartilage. The use of these appropriate reference genes will facilitate accurate gene expression studies of porcine articular cartilage and suggest appropriate housekeeping genes for articular cartilage studies in other species.     

小儿手足口病辨证论治方案专家问卷调查分析

〔摘要〕目的明确小儿手足ii病辨证论治治法、方药、内服、外用、中药注射液,为进一步制定科学、客观的小儿 辨证论治方案提供参考、方法根据文献研究结果,通过德尔菲法制定问卷,利用SPSS软件对数据进行积极度、集中 程度、协调程度、权威程度的统计与评价〔结果专家对7个证候对应的治法、方药协调程度一致,对2个中药注射 液、4个}}服中成药、3个外用中成药协调程度一致、结论专家问卷调查是分析小儿辨证论治方案的有效方法、     

料槽的调控——时时观察，拍照记录

建议对每栋猪舍经常性拍照记录以便有效地调控料槽设置量，同时确保猪群的大小与其采食空间合适。 表1列出了猪只从断奶到育肥结束期间日采食量的建议值。猪场猪只的日采食量可能由于达不到这样的量而缺乏其完全发挥生长潜力所需要的营养素。通常认为采食量达不到标准饲喂量是限制生长育肥猪生长的一个主要因素。     

Experimental Investigation on the Aseismic Behavior of Seismic Damage Masonry Wall Retrofitted with Grouting and Steel Hoops

In order to accurately evaluate the aseismic behavior of the seismic damaged masonry pagodas destroyed by Wenchuan earthquake and strengthened after the earthquake, a test was conducted to acquire related parameters on seismic performance from the specimens made by masonry and retrofitted with grouting and steel hoops. Taking a seismic damaged ancient masonry pagoda in Sichuan as a case study, four kinds of primitive specimens are fabricated by simulating the conformation of the masonry pagoda. Then the primitive specimens are destroyed under cycle loads and the damaged specimens are repaired by grouting and steel hoops. Cycle controlling tests are conducted with the specimens and relevant data are acquired. Experimental results showed that grouting combined with steel hoops could improve the ductility and energy dissipation capacity of a seismic damage of brick masonry structure, but it do a little to improve the stiffness of a seismic damage structure. And the cracking load of the repaired masonry is much lower than the original undamaged structure.     

Analysis and optimization of resistive cavitation property of annular groove within relief valve

RNG k ε turbulence model is adopted to simulate the cavitating flow of annular groove within relief valve,and the influence of structural parameters of annular groove on cavititaing flow field is analyzed. The analysis results show that performances of resistive cavitations increase with the rising of structural parameter θ when structural parameters R1and R2 are the optimal values. According to numerical simulation data of annular groove’s structural parameters,the surrogate models is obtained via the kriging calculated method,i.e. the objective function is the maximum gas volume fraction,the correlation function is the Guass function and constant regression model is applied to the likelihood function of surrogate models. The surrogate function is optimized by the improved PSO algorithm,and resistive caviatation property of the optimized structural parameters has higher efficiency compared with other ones.     

清肝宁肺方治疗嗜酸粒细胞性支气管炎 肝火犯肺证的临床观察

[摘要〕目的观察清肝宁肺方对嗜酸粒细胞性支气管炎(Eosinophilic bronchitis EB )致慢性咳嗽肝火犯肺证的 疗效,寻求中西医治疗EB的新方法及其机制、方法60例EB中医辨证肝火犯肺患者,随机分为两组各30例,治疗 组予服用清肝宁肺方,对照组吸入普米克气雾剂〔疗程1个月、治疗前后记录咳嗽症状、咳嗽相关生活质量、分析诱 导痰细胞学〔结果治疗组和对照组咳嗽症状总有效率分别为93.3%和86.7 0Ic,两组比较差异无统计学意义((P> 0.05);治疗后诱导痰嗜酸粒细胞比例转为正常者治疗组21例((70.0%),对照组13例((43.3%),两组比较差异有统计 学意义(P<0.05 );两组对咳嗽相关生活质量总有效率分别为93.3%和73.30Ic,两组比较差异有统计学意义((P<0.05 ) 治疗期间治疗组未发现有副作用者;对照组有2例患者出现咽喉轻度不适〔结论清肝宁肺方与普米克气雾剂均可 改善EB患者临床症状,但抑制EB的气管炎症、提高咳嗽相关生活质量治疗组优于对照组,且其副作用较普米克 气雾剂少、     

湿热颗粒抗炎、止痒的实验研究

〔摘要〕目的研究湿热颗粒抗炎i1=痒的作用、方法通过湿热颗粒对二甲苯所致小鼠耳廓肿胀模型、角叉菜胶 所致大鼠足踌肿胀模型的影响,观察湿热颗粒对炎症的影响;通过湿热颗粒对葡聚糖-40所致的小鼠瘤痒模型、磷 酸组胺豚鼠致痒模型的影响,观察湿热颗粒对瘤痒的影响〔结果与模型组比较,湿热颗粒各剂量组能显著降低二甲 苯所致的小鼠耳廓肿胀度,差异具有统计学意义(P<0.05 ) ;湿热颗粒中剂量组能显著降低角叉菜胶致炎后 30 min, 1,2,3,4,6 h大鼠足趾肿胀率,差异具有统计学意义(P<0.05 ) ;湿热颗粒中剂量、高剂量组能显著延长葡聚糖-40所 致小鼠瘤痒的潜伏期及减少其瘤痒次数,差异具有统计学意义(P<0.01) ;湿热颗粒中剂量、高剂量组能显著增加磷 酸组胺所致豚鼠瘤痒的致痒阂,差异具有统计学意义((P<0.05)〔结论湿热颗粒具有抗炎、i1几痒的作用〔