瘦素及其长型瘦素受体在雌性小鼠体内的表达

采用免疫组织化学SP染色法对长型瘦素受体在雌性小鼠生殖周期不同阶段的下丘脑、卵巢、子宫中细胞定位和分布进行了研究,并用ELISA方法对小鼠血清中的瘦素浓度进行了检测。结果显示,下丘脑神经元胞质中有棕褐色阳性颗粒,且阳性细胞数量随妊娠日龄增加逐渐增加,妊娠期与间情期阳性细胞数差异显著(P0.05);在卵巢中,卵母细胞胞质中有长型瘦素受体表达,随卵泡的发育,卵泡细胞中免疫反应阳性细胞数量逐渐增加;在胚泡附植期,瘦素受体在子宫腺和子宫内膜上皮细胞中大量表达。妊娠期血清瘦素浓度均高于间情期,且瘦素浓度从间情期到妊娠4日龄,呈上升趋势,妊娠5日龄稍下降,后随妊娠日龄逐渐增加。结果表明,瘦素及其长型受体能够促进卵泡发育,有利于小鼠胚泡的附植。     

妊娠家兔子宫交感神经分布的变化--HRP法研究

利用辣根过氧化物酶 (HRP)顺向追踪法 ,研究了家兔子宫交感神经的分布及妊娠后的变化。结果发现 ,HRP标记的交感神经纤维分布于子宫壁各层 ,其中内膜和环行肌层最多 ,神经纤维的直径为 3～ 9μm,平均为 (5.92± 0 .2 3)μm。光镜下对子宫切片整个横断面计数发现 ,妊娠组 (妊娠 18d)与非妊娠组相比 ,妊娠子宫各段 HRP标记神经纤维数量显著下降 (P<0 .0 1) ,表明妊娠子宫交感神经数量减少。除数量变化外 ,妊娠组子宫 HRP标记神经纤维直径也有减小的趋势。讨论了对妊娠子宫交感神经数量减少的意义     

抗鸡致病性大肠杆菌中药的筛选及其疗效观察

[目的]为获得有效防治鸡大肠杆菌病的中药制剂奠定基础。[方法]以西药盐酸洛美沙星氯化钠注射液为对照,对15味中药及7个自拟中药复方进行药敏试验,并对7个中药复方和体外抑菌效果较好的单味中药进行体内试验。[结果]药敏试验结果表明,15味单味中药均有一定的抗大肠杆菌作用,其中以芦荟、石榴皮、地榆等抑菌效果明显。7个自拟复方中复方一、复方二和复方五的抗大肠杆菌效果较好。体内试验结果表明,中药对人工感染大肠杆菌的鸡只治疗效果优于西药,以复方一和复方二效果较好。[结论]中药制剂对鸡大肠杆菌病具有较好的治疗效果,但其在体内与体外的抑菌作用机制有所不同。     

VIP免疫反应神经纤维在大鼠和小鼠子宫中的分布

应用免疫细胞化学技术,对大鼠和小鼠子官VIP免疫反应神经纤维的分布进行了观察。结果发现,大鼠和小鼠子宫均含有丰富的VIP免疫反应神经纤维,子宫壁各层均有分布,血管层和内膜中最多。在子宫角、子官体和子宫颈间未见明显差异。VIP免疫反应神经纤维的直径大部分为3～7μm,在肌层和内膜还见到直径6～9μm,甚至16μm以上短而粗的纤维束。VIP免疫反应神经纤维在子宫壁血管旁、上皮下和肌束间均有分布,提示VIP作为神经递质可能参与子宫功能活动的调节。     

生化黄腐酸添加剂对生长兔促增重效果试验

该试验检验了生化黄腐酸添加剂饲喂生长兔的增重效果。结果表明,饲喂黄腐酸添加剂对生长兔的增重具有明显的促进作用(P<0.05)。     

抗鸡致病性大肠杆菌中药的筛选及其疗效观察(英文)

[Objective] The aim of this study was to lay a foundation for obtaining effective traditional Chinese medicine preparations controlling chicken colibacillosis. [Method] With the western medicine Lomefloxacin Hydrochloride and Sodium Chloride Injection as the control, drug sensitivity tests of 15 single traditional Chinese herbs and seven self-made Chinese herbal compounds were conducted. Subsequently, the single traditional Chinese herbs presenting good in vitro antibacterial effects against Escherichia coli and all the seven compounds were given to the chicks artificially infected with E.coli to observe their efficacies. [Result] The results of drug sensitivity tests showed that all the 15 traditional Chinese herbs had certain antibacterial effect against E.coli, among which aloe, pomegranate rind and radix sanguisorbae were the best. Compound 1, 2 and 5 had good antibacterial effects in vitro. By in vivo test, traditional Chinese medicines showed superior efficacies on infected chicks than western medicine, and compound 1 and 2 performed well. [Conclusion] Traditional Chinese medicine preparations are good at controlling Chicken colibacillosis, but their bacteriostasis mechanism in vivo was different with that in vitro.