Advanced cardiac morphogenesis does not require heart tube fusion

The bilateral cardiac mesoderm migrates from the lateral region of the embryo to the ventral midline, where it fuses to form the primitive heart tube. It is generally accepted that migration and fusion are essential for subsequent stages of cardiac morphogenesis. We present evidence that, in Foxp4 mutant embryonic mice, each bilateral heart-forming region is capable of developing into a highly differentiated four-chambered mammalian heart in the absence of midline fusion. These data demonstrate that left-right chamber specification, cardiac looping, septation, cardiac myocyte differentiation, and endocardial cushion formation are preprogrammed in the precardiac mesoderm and do not require midline positional identity or heart tube fusion.     

五峰县农业环境污染现状与综合治理对策

五峰是一个农业县,具有得天独厚的自然生态环境和丰富的农作物资源。虽然经过近30年的发展,摆脱了农产品短缺的长期困扰,但随着农业集约化、专业化和区域化程度的不断提高及养殖业的快速发展,化肥、农药、农膜、畜禽粪便及污水等农业投入和农业副产品大量流失进入各类环境介质,造成的农业环境污染问题越来越突出,严重影响农业生产安全及农产品质量安全。因此,科学合理使用农业投入品,综合防治农业环境污染,全面提升农产品产地环境质量,确保农业生产安全是各级政府、部门亟待解决的问题。     

一例猪肺疫合并圆环病毒感染的诊治

本文介绍了一例猪肺疫合并圆环病毒感染的诊治。     

谈后非洲猪瘟时期养猪业振兴

随着我国非洲猪瘟疫情得到进一步控制,后非洲猪瘟时期如何养好猪,本文从生猪养殖业规划和布局,遗传资源梳理和保护,引导养殖场户尽早形成养殖、屠宰、加工一体化规模生产,推广生猪养殖福利事业4方面进行探讨,为生猪养殖振兴和发展提供借鉴和参考.     

影响根癌农杆菌介导香蕉胚性悬浮细胞遗传转化的因素

用含质粒载体pCAMBIA1301的根癌农杆菌EHA105转化贡蕉(Musa A A Pisang Mas cv. Mas)品种的胚性悬浮细胞,通过测定GUS基因瞬时表达率,对转化初期的主要影响因素进行了研究。结果表明,取处于对数期的胚性悬浮细胞和D600nm为0.2的菌液混合,在室温、8000r/min离心5min,然后在110r/min的旋转摇床上摇5min,在体细胞胚诱导培养基上共培养8d,瞬时表达率达到最高值25.30%。     

鸽鼠伤寒沙门氏菌血清凝集方法的建立

本文描述了鸽鼠伤寒沙门氏菌血清凝集方法的建立步骤,并对此方法的可靠性和在实际生产中的可操作性作了简要分析。     

马铃薯愈伤组织低温保存的生理变化

对马铃薯愈伤组织在常温(23～25 ℃)和低温(4 ℃)下保存90 d过程中所发生的部分生理变化进行了研究.结果表明,在低温条件下,随着保存时间的延长,SOD活性逐渐下降,但下降幅度明显低于常温条件;POD活性、Pro及可溶性糖含量随着保存时间的延长先上升后下降,POD活性明显低于常温条件,但Pro及可溶性糖含量明显高于常温条件.     

Identification of 5,7,3',4'-tetramethoxyflavone metabolites in rat urine by the isotope-labeling method and ultrahigh-performance liquid chromatography-electrospray ionization-mass spectrometry

5,7,3',4'-Tetramethoxyflavone (TMF), one of the major polymethoxyflavones (PMFs) isolated from Kaempferia parviflor , has been reported possessing various bioactivities, including antifungal, antimalarial, antimycobacterial, and anti-inflammatory activities. Although several studies on the TMF have been reported, the information about the metabolism of TMF and the structures of TMF metabolites is still not yet clear. In this study, an isotope-labeling method was developed for the identification of TMF metabolites. Three isotope-labeled TMFs (5,7,3',4'-tetramethoxy[3'-D(3)]flavone, 5,7,3',4'-tetramethoxy[4'-D(3)]flavone, and 5,7,3',4'-tetramethoxy[5,4'-D(6)]flavone) were synthesized and administered to rats. The urine samples were collected, and the main metabolites were monitored by ultrahigh-performance liquid chromatography-electrospray ionization-mass spectrometry. Five TMF metabolites were unambiguously identified as 3'-hydroxy-5,7,4'-trimethoxyflavone, 7-hydroxy-5,3',4'-trimethoxyflavone sulfate, 7-hydroxy-5,3',4'-trimethoxyflavone, 4'-hydroxy-5,7,3'-trimethoxyflavone, and 5-hydroxy-7,3',4'-trimethoxyflavone.