大麻GRAS转录因子家族的全基因组鉴定及镉胁迫下表达分析

GRAS转录因子在植物生长发育及抗逆境胁迫中具有重要作用。本文对大麻GRAS转录因子家族进行了全基因组鉴定,对其理化性质、系统进化发育、基因结构、基因间的共线性关系进行了分析,并利用云麻1号及内蒙古小粒大麻的转录组数据分析其在镉胁迫下的表达量变化。结果表明,大麻基因组中鉴定出54个GRAS基因, 96.30%的基因编码酸性蛋白,长度为415～757aa,分子质量为46,405.05～85,748.52kD,等电点为4.77～8.54,划分为9个亚家族,其中PAT1、LS、SHR、HAM保守性较高, PAT1、LISCL、CsGRASA出现大量基因串联重复, CSGRAS12基因在5种植物的共线性分析中均存在。将2个大麻品种进行镉胁迫处理发现,云麻1号株高下降10.48%,鲜重下降6.33%;内蒙古小粒大麻株高下降66.07%,鲜重下降42.67%,表明云麻1号较内蒙古小粒大麻更耐镉胁迫。云麻1号的54个GRAS基因中, 42个(77.78%)基因表达上调1.05～18.10, 11个(20.37%)基因表达下调0.13～0.91;内蒙古小粒大麻的54个GRAS基因中, 27个(50.0...     

工业大麻纤维负载纳米银复合材料制备及抗菌性评价

研究在不添加还原剂的条件下,利用工业大麻纤维的羟基及衍生醛基将纤维上吸附的银离子原位还原生长成纳米银颗粒,制备工业大麻纤维负载纳米银复合功能材料.通过扫描电子显微镜(SEM)、透射电镜(TEM)、X射线光电子能谱(XPS)、X射线衍射(XRD)对复合材料的形貌结构进行表征.结果表明:银离子被大麻纤维还原为纳米银并均匀地...     

利用ISSR分子标记分析44份红麻种质资源的遗传多样性(英文)

[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular marker-assisted breeding of kenaf. [Method] Ninety one ISSR molecular markers were used for amplification on 44 shares of kenaf germplasm resources, of which 21 showing good diversity and clear bands were chosen for PCR amplification. Based on amplification results, the genetic similarity coefficients among kenaf germplasm resources were calculated by using analytic software NTSYSpc-2.10e, and phylogenetic tree was then established via UPGMA. [Result] Totally 169 bands were amplified using the 21 screened primers, averagely 8.05 bands were amplified from each primer. Of them, 141 bands were polymorphic, accounting for 83.4%. When genetic similarity coefficient 0.887 was used as criterion L1, these 44 shares of kenaf germplasm could be classified to be 32 shares of cultivars and 12 shares of wild type or half-wild type varieties. When genetic similarity coefficient 0.897 was used as criterion L2, these 32 shares of cultivars could be further grouped into four sub-clusters. The genetic diversities between cultivars and wild type or half-wild type varieties were between 0.46-0.91, showing huge hereditary difference; while that among 32 cultivars were between 0.85-0.97, suggesting that genetic relationships among cultivars are relatively close and their genetic similarities are rather narrow. [Conclusion] ISSR could well determine the genetic similarities among kenaf germplasm resources and provide valuable molecular information for selecting parents of hybrid cross, which can lay a good foundation for DNA mapping of kenaf germplasm resources.     

红麻HcMYB3基因克隆和表达分析

为探究HcMYB3基因在红麻中的潜在功能和盐胁迫响应规律,研究以红麻品种“中红麻21号”为试验材料,对HcMYB3基因进行同源克隆,并进行生信分析以及盐胁迫下时空表达模式分析。结果表明,HcMYB3基因包含675 bp的开放阅读框,编码224个氨基酸,编码的蛋白质为酸性,具有不稳定、亲水性、不跨膜、不含信号肽的特性,二级结构以无规则卷曲为主,与木槿HsMYB3亲缘关系最近。HcMYB3基因在中低浓度盐(50、150 mmol/L NaCl)胁迫下显著上调,高浓度盐(250 mmol/L NaCl)胁迫下随时间呈“降—增—降”的趋势。研究为红麻耐盐调控网络的研究提供了理论基础。     

大麻FT同源基因CsHd3a的克隆及表达谱分析

为探究FT基因在大麻光周期途径中调控开花的作用,从工业大麻品种庆麻1号(Q1)中使用PCR技术克隆了FT同源基因cDNA序列,命名为CsHd3a.利用生物信息方法对该基因序列特征进行了分析,并使用实时荧光定量(qRT-PCR)技术研究其组织特异性表达模式.选取了晚花品种云麻7号(Y7)与早花品种Q1使用qRT-PCR技...     

甘蔗品种比较试验初报

经过对甘蔗品种的比较试验,选育出适应当地的优良品种.     

甘蔗品种比较试验初报

经过对甘蔗品种的比较试验,选育出适应当地的优良品种。     

红麻高产杂优组合H116多代利用的研究

本文从产量性状、抗病性和纤维品质等方面分析了红麻高产杂优组合H116（7804×南选）F#-1和F#-2的优势表现。与大值亲本7804品种比较,F#-1和F#-2的纤维产量分别平均增产21.7%和10.7%,F#-2优势降低9.1%。而与生产上大面积栽培的青皮3号品种比较,F#-1和F#-2分别平均增产30.1%和18.3%。产量差异均达到极显著或显著水平。实验证明,用两个遗传组成不同的优良品种配成的强优势组合,不仅F#-1可以利用,而且有的F#-2仍保持有较强的杂种优势,表现抗病、高产、优质,具有生产实用价值,而F#-3的利用价值不大。     

优良红麻：中杂红316

该品种前、中期生长较快,后期生长稳且不早衰;茎秆硬挺,株型紧凑;耐涝抗倒,抗病性强,适于在高肥条件下种植。其突出特点是生长快、植株高大粗壮、有效株数多、竿麻率低、生物产量高,表现为纤维产量和茎秆产量高;纤维品质好,韧皮含量高,适于纺织和造纸兼用;