军曹鱼MHC-Ⅱα基因全长cDNA的克隆及其组织表达分析

本研究根据其他鱼类MHC-Ⅱα基因的保守序列设计兼并引物,运用同源克隆和末端快速扩增方法扩增了军曹鱼MHC-Ⅱα基因的全长cDNA序列,并对cDNA及其氨基酸序列进行了分析,比较了军曹鱼和其他物种的MHC-Ⅱα氨基酸序列的差异,分析了军曹鱼MHC-Ⅱα基因的组织分布及经LPS刺激后头肾组织中MHC-Ⅱα基因的表达变化。结果表明,军曹鱼MHC-Ⅱα cDNA全长998 bp,包括53 bp的5′末端非编码区（5′UTR）、234 bp的3′末端非编码区（3′UTR）及711 bp的开放阅读框（ORF）,编码236个氮基酸,其蛋白质分子质量约为25.94 ku,等电点为4.39;军曹鱼MHC-Ⅱα蛋白质序列具有一些重要的特征,包括前导肽、α1、α2、CP/TM/CYT区和保守的半胱氨酸等;军曹鱼MHC-Ⅱα与鼠、人及其它鱼类的氨基酸同源性在25.0%～69.5%之间。Real-time PCR检测结果显示,MHC-Ⅱα基因在正常军曹鱼组织中均有表达,但其表达量在各种组织中存在差异,其中较强表达于头肾、鳃,中等程度表达于脾脏、肠,在心脏、脑、肌肉中表达较弱;经LPS刺激后,头肾中MHC-Ⅱα基因表达下调。     

基于流场特性的液力缓速器叶栅角度优化设计

为了提高液力缓速器制动转矩系数,对液力缓速器内湍流流动进行了三维瞬态多相流数值模拟。通过对其内流场特性分析,发现液力缓速器工作流道的壁面脱离现象以及弦面内产生的涡旋区不利于恒定制动转矩的输出。针对这一问题,考虑到液力缓速器各结构参数之间的相关性,对多参数共同作用的内流场进行数值分析,优化叶栅参数。结果表明,通过分析多参数共同作用的流场来优化缓速器叶栅参数的方法比较合理;对液力缓速器叶片前倾角和叶片前缘倒角进行优化后,叶轮流道内存在的涡团和流动分离现象基本消失,制动转矩系数提高6%。     

Dynamic analysis and optimization of gear trains in a megawatt level wind turbine

Through coupling vibration analysis of the transmission system of a 1.5 MW wind turbine gearbox, a torsional vibration model of a gear drive system in wind turbines is established. The fourth order Runge Kutta integration method is applied to calculate the dynamic response of a gear system caused by wind loads, time varying meshing stiffness, and system damping. The harmonic balance method is used to find the analytic solution. Based on the solution, a mathematical model for optimum gear system design is established. The model aims at minimizing the vibration acceleration of the planet gear and the total mass of the gear transmission system. The optimization toolbox in MATLAB program is adopted to obtain the optimal solution. A calculated example shows that the primary natural frequency of the gear system has been raised, the dynamic properties of the gear train have been improved remarkably, and the total mass of the gear train has been decreased.     

Plastic upper bound theory reliability analysis of coal gangue hills

We established the plasticity limit analysis reliability limit state equation and the reliability calculating formula for gangue hills using the JC method. This work was based on the plasticity mechanics limit analysis upper bound theory and traditional slice methods. A reliability index of gangue hill stability was derived using this equation. It was found that the safety coefficient of gangue hills derived by the limit state equation risks failure if the variability of cohesion and internal friction angle are not considered. When the variability of the strength parameters rises, the reliability theory more consistently projects practicality than the limit state equation for gangue hill stability assessment.     

contact stress and steady thermal analysis of herringbone gears in a gas turbine

To obtain the distribution characteristic of contact stress and the bulk temperature on the surface of herringbone gears, parameter modeling of gears was carried out by parametric design language. A numerical simulation was given to calculate the contact stress field and the steady thermal field by combining heat transfer theory, tribology theory, and mesh theory. The distribution of contact stress, the bulk temperature and heat flux also were analyzed. The analysis results show that the distribution of the load along the contact line possesses regularity. The heat flux along the tooth depth direction decreases first and then increases. The effect of thermal conduction of the tooth is stronger than that of convective heat transfer. The conclusion indicates that the finite element emulation method can be used to estimate the transmission performance of herringbone gears.     

Agouti基因及其与动物毛色关系的研究进展

动物特别是毛皮动物,毛纤维颜色丰富多彩,其主要由遗传基因调控,在众多的调控基因中,Agouti基因是一个重要的功能基因。论文重点综述了Agouti基因结构及其在人、小鼠、家养普通动物和毛皮动物毛色方面的研究现状,旨在为动物毛色选育提供参考依据。     

基于GF集理论的五自由度混联机器人构型综合

基于GF集理论提出了一种简单而有效的混联机器人构型综合方法。首先阐述了GF集的基本概念、运算法则及转动特征存在条件。其次通过分析混联机构结构组成特点,提出了基于GF集元素组合和转动轴线迁移定理安排混联机构拓扑结构的方法,建立了混联机构数综合方程并给出了混联机构构型综合的具体步骤。基于该构型综合理论,综合出了具有确定运动特征的3T2R混联机构。针对综合出的混联机构,提出了一种混联机构末端运动特征的分析方法,运用该方法分析了一种综合得到的3PRPaRRR混联机构,证明了该构型理论的正确性。设计出的3PRPaRRR混联机构具有结构紧凑、定位精确、高速等优点,同时给出了实例应用。     

Study on Intelligent Displaying Mechanism of Control Fissure of Perilous Rock

Perilous rock is a kind of typical disaster and characterized by multitudinous structural planes; when the disaster happens, only one of the planes is the control fissue and the others are secondary. In this paper, the authors have done some attempts for     

Landfill Leachate Treatment by Cavitating Water Jets

Cavitating water jets were used to degrade complicated organic compounds sampled from landfill leachate from a sanitary landfill in Chongqing, P. R. China. The law of chemical oxygen demand (COD) and the impact of factors such as pumping pressure, confining pressure, cavitating time and pH were evaluated in the experiment. We concluded that a cavitating water jet can degrade complicated organic compounds. Cavitating water jets therefore can improve biological oxygen demand (BOD)5/COD. The optimal cavitating condition was also obtained: at a pH 9.0, confining pressure 0.6MPa, cavitating time 90mins and pumping pressure 10MPa,the COD, BOD5 and BOD5/COD increase to 124.8%, 293.3% and 52.44% respectively. Chromaticity decreases while SS increase to 191.5%. These changes create good conditions for subsequent treatment.     

A cusp catastrophe model study of destabilization of soft sandwich rock slope

A slope geomechanical model was proposed based on the destabilization characteristics of soft sandwich rock slopes. Based on an analysis of the effects of internal external factors on slope stability, the cusp catastrophe model of slopes can be employed to analyze and predict the necessary conditions leading to slope catastrophes. During the processing of the mass and energy exchange with the external, this may lead to the change of controlling variables. Slides or quick or slow creeping thus may take place due to environmental complexity. This work helps deepen understanding of the formation of landslides and makes feasible the applcation of the catastrophe model to slope destabilization investigations.