杧果短截结果母枝玉米素核苷含量的动态变化

【目的】测定杧果花芽分化期间结果母枝各器官内玉米素核苷（ZR）含量,探索ZR在杧果花芽分化中的作用。【方法】利用杧果花枝短截后剪口芽在当年还能再次花芽分化并开花结果的现象,应用酶联免疫吸附分析方法（ELISA）定期测定剪口叶、附近韧皮部及芽内ZR含量。【结果】短截结果母枝后,剪口叶、附近韧皮部和剪口芽内ZR含量分别于2月25、20日和3月2日降至最低,为1.73、1.52、2.0 μg·g-1FW;叶片、韧皮部及剪口芽于3月12日达最大值,为6.39、8.47、10.32 μg·g-1FW。2月25日剪口芽芽眼中心颜色逐渐呈蜡黄,并向芽体四周扩散;3月2～7日,芽体逐渐膨大,蜡黄色加深,芽眼呈半透明状;3月7日后绿色逐渐增加,半透明状消失。【结论】ZR在杧果花芽分化的不同阶段具有不同作用。花芽分化临界期前,ZR含量低,生长点细胞活性下降,有利于杧果植株从叶芽生理状态转化为花芽生理状态;花芽临界期后,相对高的ZR含量有利于杧果植株生长点进行花芽分化。     

鸡非典型新城疫的综合防控

鸡新城疫(AvianNewcastleDisease,ND)是由新城疫病毒(Newcastlediseasevirus,NDV)引起的一种高度接触性、败血性传染病,主要特征是下痢、呼吸困难、神经机能紊乱、黏膜和浆膜出血。随着养鸡业免疫技术的发展和饲养管理水平的不断提高,典型新城疫得到了很好的控制,但非典型新城疫却屡屡发生,是危害     

Oxidant-antioxidant imbalance in horses infected with equine infectious anaemia virus

This study assesses the impact of equine infectious anaemia virus (EIAV) infection on the oxidant/antioxidant equilibrium of horses. Blood samples from 96 Romanian horses aged 1-25 years, were divided into different groups according to their EIAV-infection status, age, and time post-seroconversion. The effect of infection on oxidative stress was estimated by measuring enzymatic antioxidants (superoxide dismutase [SOD], glutathione peroxidase [GPx] and catalase), non-enzymatic antioxidants (uric acid and carotenoids), and lipid peroxidation (malondialdehyde [MDA]). Infection modified the oxidant/antioxidant equilibrium in the horses, influencing GPx and uric acid levels (P<0.05). Time post-seroconversion also contributed to oxidative stress imbalance, exhibiting a significant influence on both SOD and MDA concentrations in the blood (P<0.05). Animal age did not have a significant influence on oxidative stress. Recently infected horses (<1 year following seroconversion), and horses >5 years old, represented the most vulnerable category in terms of oxidative stress, followed by recently infected animals <5 years old. The results of this study are novel in implicating EIAV infection in the development of oxidative stress in horses.     

掌叶覆盆子红熟果营养品质及耐贮性测定

以淳安产掌叶覆盆子红熟果为试验材料,对其可溶性固形物、Vc、总糖、总黄酮等含量进行分析测定,结果表明,其营养成分和生物活性物质含量较高。红熟果平均可溶性固形物、可溶性总糖和可滴定酸含量分别为13.68%、111.8 mg·g^-1和1.27%,糖酸比10∶1左右;Vc、总黄酮含量可达1.59和1.79 mg·g^-1,鞣花酸、山奈酚-3-O-芸香糖苷含量分别为0.038%、0.043%,具有较强的抗氧化能力。红熟果采后放入4 ℃冰箱内,18 h 总黄酮、可溶性固形物和Vc含量开始加速下降,48 h后烂果率过高不宜食用。     

基于RAPD和EST-SSR标记的秀珍菇菌株聚类分析

本研究利用RAPD标记和EST-SSR标记对10个不同来源的秀珍菇菌株进行聚类分析。200条RAPD引物中127条有扩增产物,引物可用率为63．5％。从NCBI数据库中下载秀珍菇及相近侧耳属食用菌EST序列2167条,聚类比对后得到全长为713．541kb的非冗余EST1442条,搜寻后得到的29个EST-SSR全部设计引物,其中26对引物（89．7％）显示多态性。根据17条RAPD核心引物和10对EST-SSR核心引物的扩增结果,对10个秀珍菇菌株进行了RAPD标记、EST-SSR标记及二者相结合的聚类分析。3种分析结果相近,且与菌丝、子实体生长特性分析结果相统一——10个供试菌株区分为5组：1～4号菌株为一组,6号和7号菌株为一组,8号和9号菌株为一组,5号和10号菌株各自为一组。     

免疫诱抗剂保康灵1号对葡萄促生长与抗病的效应

以浙江省葡萄主栽品种甬优1号为试材,分别用保康灵1号（BKL1）、化学农药和清水处理后测定葡萄叶片面积、厚度、叶绿素含量、苯丙氨酸解氨酶活性、果实重量和总糖含量以及葡萄霜霉病和炭疽病的发病率等指标。结果表明,相比清水对照,BKL1处理能增加葡萄叶片面积和厚度,提高叶绿素含量和苯丙氨酸解氨酶活性,增加葡萄果实重量,并能提高葡萄果实总糖含量,减少葡萄霜霉病和炭疽病的发病率。除叶片面积、霜霉病和炭疽病的发病率外,BKL1处理在其他指标上均优于化学农药。总之,BKL1能改善葡萄的生长系统,促进葡萄增产和品质改善,并能激活葡萄的免疫系统,增强葡萄抗病能力。     

鄞州区土地利用变化及其生态效应

摘要：基于鄞州区2000,2003,2007年3期的土地利用变更调查数据,利用土地利用动态变化模型、生态效应评价模型以及主成分分析方法,定量分析了鄞州区2000-2007年土地利用动态变化特征及其驱动因素,并衡量了土地利用变化所引起的生态服务价值及碳排放的变化。结果表明,2000-2007年鄞州区土地利用发生了显著变化,耕地和未利用地大量减少,居民点及工矿用地、交通运输用地等各类建设用地大面积增加。GDP增长、固定资产投资、产业结构变化、人口增长等社会经济发展因素与土地利用变化密切相关,是区域土地利用变化的主要驱动因素。从生态系统服务价值变化来看,虽然森林生态系统的生态服务价值略有增长,但区域总体生态系统服务价值呈减少趋势。从碳排放变化来看,建设用地作为主要的碳源,2003-2007年间碳排放量大幅增加,导致区域总的碳排放量翻了一番。建议加强生态用地保护,提高存量建设用地集约利用水平,减少碳排放。     

Chlamydial infections in duck farms associated with human cases of psittacosis in France

Five severe cases of psittacosis in individuals associated with duck farms were notified in France between January and March 2006. Diagnostic examination included serology and/or molecular detection by PCR from respiratory samples. As a consequence, we investigated all duck flocks (n=11) that were housed in the three farms where human infections occurred. While serology by complement fixation test was negative for all samples, cloacal and/or tracheal chlamydial excretion was detected by PCR in all three units. Notably, one duck flock was tested strongly positive in 2 of the 3 affected farms, and Chlamydophila (C.) psittaci strains were isolated from cloacal and/or tracheal swab samples from both farms. Human samples and duck isolates exhibited the same PCR-RFLP restriction pattern, which appeared to be an intermediate between genotypes A and B. Analysis of ompA gene sequences and comparison to those of the type strains showed that the isolates could not be strictly assigned to any of the generally accepted genotypes of C. psittaci. Further analysis by MLVA of the PCR-positive human samples revealed two distinct patterns, which were related to previously isolated C. psittaci duck strains.     

Microstructures and tensile properties of commercial AZ31B magnesium alloy sheets

Uniaxial loading tests are performed on 0.8 mm thick sheets of commercial AZ31B magnesium alloy in the temperature range from room temperature to 400 ℃. The microstructures and tensile properties are studied. The results reveal that there are few twins in the deformation microstructures created when deformation occurres in temperatures ranging from room temperature to 100 ℃, and dynamic recrystallization take place at deformation temperatures exceeding 200 ℃. Commercial AZ31B magnesium alloy sheets have a preferable strength and ductility at room temperature. The ductility of the sheets is improved as the deformation temperature increases. Some characteristics of low temperature superplasticity or high strain rate superplasticity exist.     

草莓工厂化育苗基质的筛选

以东北泥炭土为主分,辅以草莓种植地来源广泛的工农业废弃物,配制成8种不同配方的复合基质。通过对复合基质进行理化性质测定、观察其EC和pH值在育苗过程中的变化、分析所育子苗的生物学性状和第1批产果情况,并进行模糊综合评判,筛选出适合草莓无土育苗的基质配方为泥炭∶砖粒∶砻糠灰（3∶1∶1）和泥炭∶砻糠灰（1∶1）。