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仔猪黄痢是由大肠杆菌引起的一种急性肠道传染病。多发生于一周龄以内的初生仔猪,以排黄色粘液性粪便为特征。该病发病急、传播快、死亡率高,常由一头开始,迅速蔓延全南。笔者从1999年3月份起对48窝黄痢仔猪有计划地分别给以不同药物治疗,其结果报告如下:1选择药物25%恩诺沙星注射液,广东省海康兽药厂生产,批号98x34146;三九克痢注射液,惠洲三九新兽药开发有限公司生产,批号980218;科星注射液,湖南省湘潭县畜牧局中农大兽药厂生产。2用法及用五三个分娩间各12张分娩床,分娩时间3月15日一5月25日。在饲养管理条件相同的情况下… 相似文献
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本研究利用EST-SSR分子标记,对国内的52份老芒麦(Elymus sibiricus)材料进行遗传多样性的研究,并构建了7个老芒麦品种及栽培材料的DNA指纹图谱。20对EST-SSR引物共产生204个条带,平均每对引物扩增出10.2条带,176(86.27%)条带为多态性条带,表明供试材料具有较高水平的多态性。同时,引物Elw404和Elw195构建的指纹图谱较为容易地将品种和栽培材料与其他材料区别开。分子方差分析(AMOVA)表明,老芒麦地理区域内(73.94%)的遗传变异远高于地理区域间(26.06%)。结构分析将52份材料分为5组,主成分分析(PCoA)结果与之相似。与品种及栽培材料相比,野生材料具有更高的遗传多样性[多态性条带数(NPB)为174,多态性百分比(PPB)为85.29%,香农多样性信息指数(I)为0.296 6,Nei’s基因多样性(H)为0.179 8,观察等位基因数(Na)为1.852 9],可作为重要的遗传资源用于后续的育种工作。本研究结果表明,EST-SSR分子标记可以有效地评价老芒麦种质遗传多样性及进行品种鉴定。研究结果为制定老芒麦种质原位和非原位保护策略提供参考。 相似文献
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In this study,SCoT molecular markers were used to analyze the genetic diversity and construct DNA fingerprints of three species of Elymus on the Qinghai-Tibet Plateau to provide a theoretical basis for identification of the different species in collection material. Among 80 SCoT primers, 22 primers were selected for PCR amplification,and a total of 290 bands were amplified,of which 254 were polymorphic with the percentage of polymorphic bands(PPB)of 87.59%. The average values of the Shannon diversity information index(I),Nei’s gene diversity index (H),the observed number of alleles (Na),and an effective number of alleles (Ne) were 0.5411,0.3643,1.9856,and 1.6270,respectively. Cluster analysis results showed that the genetic similarity coefficient ranged from 0.50 to 0.80. At a genetic similarity coefficient of 0.53,46 collections could be divided into 2 groups,and the results of the principal coordinate analysis were consistent with those of cluster analysis. At the same time,the DNA fingerprints of 46 materials were constructed by using 4 SCoT primers. The SCoT molecular marker was suitable for genetic diversity analysis and DNA fingerprint construction of Elymus germplasm resources. This study provides fundamental information for germplasm identification,high-quality character mining,and breeding practice for wild Elymus. © 2022 Editorial Office of Acta Prataculturae Sinica. All rights reserved. 相似文献