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Membrane-baesd yeast two-hybrid system is an effective method for research on interaction between Soybean mosaic virus-encoded membrane-associated proteins and host factors, while the Gateway technology without the use of restriction enzyme cloning techniques is easier for construction of virus-induced host cDNA library. In this study, both membrane-based yeast two-hybrid system and GatewayTM systems were used. With TRIZOL regent, total RNA was extracted from soybean leaves infected with soybean mosaic virus. SMV-induced soybean primary cDNA library constructed by Gateway technology was recombined into a reconstructed prey vector for membrane-based yeast two-hybrid system. The capacity and quality tests showed that the library titer was 1.7 ? 106cfu/mL and the length of inserted cDNA fragments ranged from 0.5 to 2 kb. It is available for research on interaction between the virus-encoded membrane protein and host. 相似文献
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豇豆是江苏省重要的豆科蔬菜作物之一,也是高效设施农业中的主要作物。常年栽培面积为4.67万~6.00 万hm2(70 万~90 万亩),其中有10%左右用于出口到日本、韩国、东南亚等地,每年可创造1亿元经济效益。虽然江苏省及周边地区豇豆栽培面积得到迅速增长,但适合于露地和大棚栽培的高产、优质、抗病、耐低温弱光的豇豆品种仍缺乏。江苏省农业科学院蔬菜研究所选育的苏豇2 号是以早豇1号为母本,以苏豇78-29 为父本,经杂交后系统选育而成的蔓生型豇豆新品种。2012 年7 月20 日通过江苏省农作物品种审定委员会鉴定。 相似文献
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苏菜豆4号是以81-6为母本,以苏地豆1号为父本杂交后经系统选育而成的矮生菜豆新品种。早熟,花紫色,嫩荚扁棍形,荚绿白色,平均荚长13.4cm,宽0.8cm,平均单荚质量7.7g。从播种至采收嫩荚56.1d(天)。每667m~2鲜荚产量800~1200kg。田间对叶霉病和根腐病的抗性强。种子为扁椭圆形,平均千粒重为333.0g。适合江苏省及长江中下游地区春秋季露地和大棚栽培。 相似文献