Application of quantum dot-antibody conjugates for detection of sulfamethazine residue in chicken muscle tissue

A new indirect competitive fluorescence-linked immunosorbent assay (cFLISA) method for the detection of sulfamethazine (SM2) in chicken muscle tissue was demonstrated using quantum dots (QDs) as the fluorescence label coupled with secondary antibody. The sensitivity of the cFLISA was compared with that of the HPLC method. The 50% inhibition value (IC50) and the limit of detection (LOD) of the cFLISA were 7.7 and 1.0 ng/mL, respectively. When SM2 was spiked at levels of 50, 100, and 200 ng/g, recoveries ranged from 80.6% to 117.4%, with a coefficient of variation of 6.9-9.6%. In the incurred sample analysis, the amounts of SM2 quantified by cFLISA were similar to the results obtained by the HPLC method. This study shows that cFLISA could be used as a screening method in practice.     

早酥红梨在甘肃正宁的引种栽培

<正>早酥红梨是早酥梨的红色芽变。我们于2013年从杨凌农高会上引进该品种试栽。2017年始结果,其果实品质优良,色泽鲜艳,具有很好的观赏和食用价值,可在甘肃庆阳地区推广。1主要性状1.1果实经济性状果实高桩,个大,色泽鲜红,具有棱状突起。平均单果质量280 g,最大570 g,果皮薄脆,果心小,可食率高,果肉莹白,质细汁多,味甜微酸,酥脆爽口,石细胞少,可溶性固形物含量11%～15%。幼果全红,成熟后果实鲜红,果面光亮,鲜艳洁净。     

Applications and prospects of functional oligosaccharides in pig nutrition: A review

Oligosaccharides are low molecular weight carbohydrates between monosaccharides and polysaccharides, which consist of 2 to 20 monosaccharides linked by glycosidic bonds. They have the effects of promoting growth, regulating immunity, improving the structure of intestinal flora, and are antiinflammatory and antioxidant. With the comprehensive implementation of the antibiotic prohibition policy in China, oligosaccharides as new green feed additive have been paid more attention. Oligosaccharides ca...     

Individual Identification of Dairy Cows Based on Deep Feature Extraction and Matching

Individual identification of dairy cows is the prerequisite for automatic analysis and intelligent perception of dairy cows’ behavior. At present, individual identification of dairy cows based on deep convolutional neural network had the disadvantages in prolonged training at the additions of new cows samples. Therefore, a cow individual identification framework was proposed based on deep feature extraction and matching, and the individual identification of dairy cows based on this framework cou...     

Pharmacokinetics of florfenicol in healthy and Escherichia coli-infected broiler chickens

The aims of this study were to evaluate the effects of a ketamine/propofol anaesthetic protocol in lions (Panthera leo), and to compare it to two commonly used anaesthetic protocols. Seventeen adult lions were anaesthetised using three different protocols. Group XK (n=6) was anaesthetised with intramuscular (i.m.) injections of xylazine and ketamine. Group KD (n=5) was anaesthetised with an i.m. injection of ketamine, followed by an intravenous (i.v.) injection of ketamine and diazepam. Group KP (n=6) was anaesthetised with an i.m. injection of ketamine followed by an i.v. injection of propofol. There was a significant difference in heart rate (P<0.0002), which was lowest in group XK and highest in KD. Jaw tone was significantly lower in Group XK (P<0.05). No undesirable effects were noted following injection of the propofol. Propofol was a suitable and safe drug for maintenance of anaesthesia in adult lions.     

Interleukin-6 expression in gut of parasite challenged sheep

Following challenge with Trichosirongylus colubrifonizis, increased numbers of T-cells and immunoglobulin responses are seen in the intestine of sheep immunised by repeated infection with live worms. IL-6 mRNA expression in the small intestine from T. colubriformis-immunised and naive sheep was determined by in situ hybridisation, whereas CD4(+), IgA(+), IgG(+) cells in the gut were evaluated by immunohistochemistry. There was constitutive expression of IL-6 mRNA by cells in the naive gut, and the number of these cells was increased by parasite challenge. There were corresponding increases in numbers of CD4(+) and TCR gamma/delta(+) T-cells and IgG(+) B-cells. Our data are consistent with a role for IL-6, perhaps produced by CD4(+) and/or TCR gamma/delta(+) T-cells or B-cells, in B-cell terminal differentiation. Infiltration of B-cells, particularly IgG(+) B-cells, may reflect parasite immunity in the host.     

Construction of cDNA Library from Populus euphratica

1 Introduction Populus euphratica is a salt tolerant tree species, which is mainly distributed in the desert regions in northwest China (Wei 1993). Moreover, it is the only large tree species, which can survive and develop into forest in these arid and saline-alkali areas. P. euphratica forest plays a very important role in restraining the expansion of desert, maintaining ecological environment in west China, protecting the biological diversity and raising the local people抯 living standard…     

Suppressive effect of a proanthocyanidin-rich extract from longan (Dimocarpus longan Lour.) flowers on nitric oxide production in LPS-stimulated macrophage cells

Anthocyanidins found in certain flowers have been shown to act as strong antioxidants in various systems, exhibiting multiple biological actions. The antioxidative effects of water extract and ethanolic extract of longan (Dimocarpus longan Lour.) flowers were evaluated by radical scavenging activity and compared to those of gallic acid, myricetin, and epigallocatechin gallate. In this study, the suppressive effects of longan flower extracts on nitric oxide and prostaglandin E2 production were investigated using a lipopolysaccharide-stimulated RAW 264.7 cell model. Abundant levels of phenolic compounds including flavonoids, condensed tannins, and proanthocyanidins were found in water or ethanolic extracts prepared from dried longan flowers. The antioxidative effect of longan flower extract was similar to the effect exhibited by pure antioxidants. Moreover, longan flower extract showed prominent inhibitory effects on prostaglandin E2 production. Significant concentration-dependent inhibition of nitric oxide production was detected when cells were cotreated with lipopolysaccharide and various concentrations of longan flower extracts. These inhibitory effects were further attributed to suppression of inducible nitric oxide synthase protein expression and not to reduced enzymatic activity. These results suggest that longan flower crude extracts, especially ethanolic extract, have antioxidant and anti-inflammatory effects, and the probable mechanism involves inhibition of inflammation by proanthocyanidins. Preliminary observations suggest that longan flower extract, especially alcoholic extract, could be another potential source of natural dietary antioxidant and anti-inflammatory agent.     

提高母猪生产效率:营养与健康

本综述主要研究能够满足猪胎儿生长发育、乳腺生长和泌乳需求的母猪营养需要和平衡。本文将讨论饲喂策略如何根据母猪的养分需要进行调节,以提高它们的生产效率和健康。本文所用的大部分研究数据是基于1996~2013年之间所进行的研究。母猪的营养需要受母猪所处的妊娠阶段和胎次的影响。母猪日粮中的抗氧化剂浓度需要进行重新评估,以防止在妊娠后期和哺乳期出现极端的过氧化应激。在饲喂母猪时,考虑妊娠母猪的饲喂阶段和哺乳母猪的胎次,能提高母猪的生产寿命和健康状况。使用精选的营养成分和添加剂,似乎能帮助母猪提高生产力和改善健康状况。