Cloning and Analysis of 5' Flanking Region of Sporamin A Gene from Sweet Potato

[Objective] The aim of the study is to clone the 5’ flanking region of Sporamin A gene from sweet potato. [Method] The sweet potato "Xushu 18" was used to amplify the 5’ flanking region of Sporamin A gene using specifically designed primers and then target fragment was analyzed by PLACE and PlantCare online. [Result] Besides the conserved elements including TATA-box and CAAT-box,the cis-acting elements including sucrose responsive element CMSRE1,SP8 acting site and some other regulatory sequence such as MYB binding site were also found in Spo A promoter sequence. The results suggest that the promoter has sucrose responsive function.[Conclusion] The study provided reference to reveal the regulation law of Spo A,and to develop high-level expression vectors promoted by Spo A promoter.     

Profiling of miRNAs in porcine Sertoli cells

Background: Sertoli cells(SCs) create a specialized environment to support and dictate spermatogenesis.MicroRNAs(miRNAs), a kind of ~ 22 nt small noncoding RNAs, have been reported to be highly abundant in mouse SCs and play critical roles in spermatogenesis. However, the miRNAs of porcine SCs remain largely unknown.Methods: We isolated porcine SCs and conducted small RNA sequencing. By comparing miRNAs in germ cells, we systematically analyzed the miRNA expression pattern of porcine SCs. We screened the highly enriched SC miRNAs and predicted their functions by Gene Ontology analysis. The dual luciferase assay was used to elucidate the regulation of tumor necrosis factor receptor(TNFR)-associated factor 3(TRAF3) by ssc-miR-149.Results: The analysis showed that 18 miRNAs were highly expressed in SCs and 15 miRNAs were highly expressed in germ cells. These miRNAs were predicted to mediate SC and germ cell functions. In addition, ssc-miR-149 played critical roles in SCs by targeting TRAF3.Conclusion: Our findings provide novel insights into the miRNA expression pattern and their regulatory roles of porcine SCs.     

湘北地区大福大根萝卜引种试验初报

通过对大福大根萝卜的引种栽培,初步了解其生物学特征、农艺性状及产量的形成等。引种结果表明,大福大根萝卜具有丰产、优质、商品性好等特点,丰产性尤为突出,总产量为47.83t/hm2,适于湘北地区大面积推广种植。     

意大利大白猪窝产仔数候选基因的遗传结构分析

试验旨在研究意大利大白猪(ITLW)窝产仔数候选基因的多态性。试验选取6个农场120头经产母猪,对10个基因的10个SNPs进行了基因分型,在其他猪中这10个基因的多态性与窝产仔数性状间具有相关性,是功能和/或染色体定位的候选基因。结果表明,10个SNPs中的CLGN、pDAZL和RFN4没有发现基因分型,剩余具有多态性的7个标记的小等位基因频率在0.10(AFP)～0.48(RBP4)之间。由于所选位点中存在基因多态性,因此,AFP、BMPR1B、CXCL10、ESR2、GNRHR、MAN2B2、RBP4可作为候选基因用于意大利大白猪窝产仔数性状的相关性研究。     

矿物质的营养、代谢及其对现代猪生产性能和环境的影响（三）

4．2.1 镁 镁对骨骼肌有松弛作用（Hubbard，1973），可以通过在日粮中添加镁降低血浆中可的松、去甲肾上腺素、肾上腺素和多巴胺的浓度帮助缓解应激（Niernack等，1979；Kietzman和Hblonski，1985）。因此，已开展了日粮中添加镁降低应激和提高猪肉质量方面的试验研究。     

新疆几种主要特色果树施肥现状调查研究

以新疆几种主要特色果树(苹果、香梨、灰枣、核桃)为研究对象,分别在阿克苏市红旗坡农场、柯克亚林管站、实验林场和核桃林场,以农户为单元进行果树施肥调查.结果表明:四种果树在基肥使用上均以羊粪为主,有时辅以少量油渣;追肥则以二胺、尿素和氨基酸铵为主;每公顷施肥量呈现苹果>香梨>灰枣>核桃的递减趋势,盛果期果树施肥养分N:P2O5:K2O依次为1:0.92:0.08、1:0.94:0.02、1:0.72:0.03和1:1.35:0.06:施肥次数和时期因当地气候和果园灌水时间而产生差异,施肥次数依次为3、4、5、3次.     

’99中国国际饲料工业新技术研讨会将在京举办

由中国饲料工业协会主办的’99中国国际饲料工业新技术研讨会将于10月27日至29日在京召开。     

全球氮肥供应及需求展望(下)

世界氮肥供应状况氮肥供应趋势过去两年中,氨与尿素价格的急剧变化已经表明了天然气与氮肥价格的相对明显的相关性。目前,世界约四分之三的氨产品来自于天然气,16%来自于煤,其主要来自分布在中国600个小型碳铵生产厂。燃油与粗挥发油在氨生产中使用有限,它们主要应用在印度。氨