Expression of a Carrot Antifreeze Protein Gene in Escherichia coli

The recombinant expression vectorpET43. lb-AFP, which contains full encoding region of a carrot 36 kD antifreeze protein (AFP) gene was constructed. The recombinant was transformed into expression host carrying T7 RNA polymerase gene (DE3 lysogen) and induced by 1 mmol. L-1 IPTG (isopropyl-β-D-thiogalactoside) to express 110 kD polypeptide of AFP fusion protein.The analysis of product solubility revealed that pET43. 1b-AFP was predominately soluble, and the expressed amount reached the maximum after the IPTG treatment for 3 h.     

Econometric Analysis of Factors Affecting Special Purpose Forests in China

1IntroductionTheforestestateisoneofthemostimportantsectorsofournaturalendowmentandisthematerialfoundationofsustainabledevelopmentinChina.TheurgentquestionfortheforestrysectorinChina,andindeedfortheentireworld,ishowtoeffectivelyincreasethesupplyofforestresourcesandmaintainthecurrentimprovementinenvironmentalconditionsinordertopromotesustainableeconomicdevelopment.Chinaisalargecountrywithcomplexnaturalconditionsandgreatclimaticdiversity,suitableformanytypesofforests.Thisvarietyandwealthofherfor…     

Study on Longitudinal Gas Permeability of Air-dried Masson Pine Sapwood

INTRODUCTION Masson pine (Pinus massoniana) is among the importantcommercial woods in China (Cheng Junqing 1984; Zheng Renhua et al. 2000). Permeability is one of the main properties of wood and has been studied in detail for wood preservation and impregnation in chemical pulping (Siau 1995). Due to its much simpler procedure, gas permeability is measured more often than liquid permeability (Siau 1995). Reports have been published concerning gaspermeability of Masson pine (Bao Fucheng…     

环境因素对6种植物扦插生根的影响

以凯旋金露梅、迎红杜鹃、黄花锦带、麦李、大花木绣球、茶条槭绿枝为研究对象,利用扦插技术,对影响插穗生根的光照强度、环境温度、插床温度、空气相对湿度等环境因素进行筛选,建立稳定扦插繁育技术体系。     

番茄SlMAPK12基因的分离及表达特征分析

利用PCR克隆技术,在番茄Micro-Tom的cDNA中分离出一个促分裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK),命名为SlMAPK12(GenBank登录号为JF795447)。该基因编码621个氨基酸,含有促分裂原活化蛋白激酶11个区域,磷酸化位点为TDY(苏氨酸-天冬氨酸-酪氨酸,属于D组)。氨基酸序列比对发现,它与拟南芥、杨树、蓖麻等D组MAPK具有高度一致性。利用RT-PCR及qRT-PCR技术分析SlMAPK12的时空表达特性,发现SlMAPK12在Micro-Tom植株的多个器官中均有表达,在雄蕊中的表达量最高。对Micro-Tom幼苗进行(38±1)℃高温处理,发现SlMAPK12在雄蕊中的相对表达量增高,于12h时点达到最高,而后下降。推测SlMAPK12可能参与番茄花粉发育过程中的高温胁迫反应。     

交配持续时间对茄28星瓢虫生殖适应性的影响

茄28星瓢虫(Henosepilachna vigintloctopunctata Fabricius)的我国茄科植物上的重要的害虫种类.本文以此虫为材料,研究了该虫的交配起始时间与交配持续时间,以及不同的交配持续时间其产卵量和孵化率的影响.结果表明,该虫的交配起始时间和交配持续时间变化幅度较大,平均分别为(36.61±3.24) min和(49.87±1.06) min,且两者间并不存在明显的相关性.雌虫的产卵量和卵的孵化率与交配持续时间之间呈明显的正相关,表明延长交配时间与雌虫贮存的精子数量相适应.同时,结果还表明,该种类至少需要10 min以上的交配才能导致卵的孵化.     

48%仲丁灵乳油防除稻田杂草效果及安全性

为探索48%仲丁灵乳油在南方稻田应用的可行性,2011年通过田间试验了该药剂对稻田杂草的防除效果及对水稻生长的安全性.结果表明,在水稻移栽后7 d 施用48%仲丁灵乳油1080~3600 g(ai)/hm2,对稻田一年生禾本科杂草稗草、马唐具有较好的防除效果,对阔叶杂草鸭跖草、节节菜和莎草科杂草异型莎草、牛毛毡具有明显的兼治作用,对水稻生长安全,可以在示范的基础上推广应用.     

白菜核雄性不育两用系生理生化特性的分析

 白菜核隐性雄性不育两用系开花期，可育株群花蕾抗氰呼吸强度显著高于不育株群；不育株群花蕾的I从缺乏，而zr盈余；过氧化物同工酶带不育株群的中花蕾和大花蕾强于可育株群；细胞色素氧化酶同功酶可育株群中的小花蕾和中花蕾的两条带强于不育株群；可育株群的中花蕾可溶性蛋白电泳表现出两条特异带，而不育株群的中花蕾有一条特异带。     

大棚绿芦笋两棚三膜覆盖促发栽培技术

浙江地区芦笋多采用设施栽培,但也会遭遇冬季和早春低温寒潮影响,导致品质变差。通过多次多点试验,发现采用地膜+中棚覆盖可以促进芦笋嫩茎提早萌发和抽升,始收期提前,且早春产量增加明显。但该技术适用于大棚芦笋留母茎栽培方式,且需要选择2年生以上的绿芦笋生产田块。