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In order to develop a rapid, sensitive and specific qPCR assay for detection and quantification of Tomato yellow leaf curl virus (TYLCV), a pair of primers and TaqMan probe were designed according to the conserved sequence of known TYLCV isolates. Combining with MNP technique, a novel MNP-qPCR detection method was established and verified based on specificity, sensitivity and reproducibility tests. The results indicated that the Ct value of plotted standard curve showed good linear relationship(R2 =0.9994)with the log of copy number of template. The established method showed a high specificity for TYLCV detection without crossing reaction with Tomato severe leaf curl virus and Tomato yellow leaf curl Sadinia virus, and was 10-fold more sensitive than routine PCR. Both coefficients of variation were less than 2%, indicating a good reproducibility. We have provided a novel method for detection of TYLCV in plant samples rapidly and quantitatively. 相似文献
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1985~2007年,22年的时间在历史长河中瞬间而过.但对于北京军犬繁育训练基地来说,22年是从无到有,艰苦创业的如歌岁月.经过22年风风雨雨的艰苦创业,北京军犬繁育训练基地由单一的军犬训练大队发展成为保障全军军犬工作开展的,集军犬繁育、训练、教学和科研为一体的综合性基地.…… 相似文献
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本研究以对牧草种植区内苜蓿(Medicago sativa)、小麦(Triticum aestivum)、玉米(Zea mays)和马铃薯(Solanum tuberosum)生产住户的实地调研数据为基础,对苜蓿及竞争农作物的投入产出进行分析。结果表明,种植苜蓿有良好的经济效益,且其潜在效益很大。本研究还通过建立苜蓿、小麦和玉米3种作物的产出模型,对其要素弹性进行分析,得出影响苜蓿及竞争农作物生产的显著要素,并提出意见和建议。 相似文献
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Francili A. Molossi Bianca S. de Cecco Camila B. Pohl Rogrio B. Borges Luciana Sonne Saulo P. Pavarini David Driemeier 《Journal of veterinary diagnostic investigation》2021,33(4):677
We determined the prevalence of diseases and pathogens associated with mortality in beef cattle in the State of Rio Grande do Sul, Brazil, based on pathology laboratory submissions. Postmortem examinations were conducted on 1,277 beef cattle that died between 2008 and 2018. Information regarding age, time of the year, breed, and regional location were analyzed statistically. Most cattle were from the surrounding region of Porto Alegre, and 78.7% of the analyzed cases had diagnostic value. The diagnostic category with most cases was infectious and/or parasitic diseases (60%), followed by toxic and toxicoinfectious (25%). Most cases occurred in the fall. Major disease conditions identified included hemoprotozoal infection (18.2%), rabies (8.2%), and plant intoxications by Senecio spp. (8.5%) and Pteridium arachnoideum (4.6%). Hemoprotozoal infection occurred at a higher frequency in young cattle, mainly in animals up to 1 y old. Intoxication by Senecio spp. was more frequent in cattle 2–3 y old. 相似文献
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This paper analyzes the limitation of regarding the weighted average cost of capital as the optimal standard of capital structure in the traditional capital structure decision-making method, and analyzes the view that the capital structure is affected by many factors,such as earning power, long-term stability of operating, the attitudes held by owners and operators, the attitudes held by creditors and financial risk. With the example, the affecting factors of the capital structure is analyzed. The fuzzy synthetic evaluation model are employed to decision-making of the capital structure. This article also shows the key point of its application. 相似文献
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In CDMA communication system, the relativities between users result in Multiple Access Interference (MAI). With the increase of users, MAI becomes the main jam of broadband CDMA communication system. Multi-User Detection (MUD) is the most important technology of anti-jamming in the broadband CDMA communication system, which can eliminates MAI effectively by using the information of all user signals to detection single. This paper analyze the expression of system capability without MUD and with MUD, and puts forward the way to increase system capability by using MUD. Then the influence of MUD on CDMA system capability is discussed by MATLAB emulation. Basing analysis and emulation, the conclusion is got: the higher the MUD efficiency, the better the improvement of CDMA system capability; under the same MUD efficiency, the lower data rate, the smaller inter-cell interference, the lower the ratio of bit energy to power spectrum, the better the improvement of CDMA system capability. 相似文献
10.
Väli Ülo Mirski Paweł Sein Gunnar Abel Urmas Tõnisalu Grete Sellis Urmas 《Landscape Ecology》2020,35(7):1667-1681
Landscape Ecology - Biodiversity is closely related to landscape heterogeneity, but both are difficult to evaluate directly. Apex predators, such as raptors, are potential surrogate indicators of... 相似文献