美洲黑杨基因资源收存及其遗传评价的研究

从１７个国家引进３３１个黑杨派无性系（其中５２个美洲黑杨无性系）,在山东省长清县营建我国第一个黑杨派无性系基因库。连续１０年对基因库内美洲黑杨无性系进行了多性状系统研究,结果表明各无性系间在物候期、生长、生根、抗寒和抗病虫等方面均存在着显著的差异,遗传变异丰富;系间的主要性状与无性系起源纬度及各性状间存在一定相关性。主要材料性状变异的研究结果表明,３８个８年生美洲黑杨无性系在木材基本密度、纤维长度无性系间变异达到极显著水平;木材密度和纤维长度与树高和胸径呈一定相关性;木材密度和纤维长度这两个性状在遗传上相互独立,受不同遗传机制控制。利用分子遗传标记ＲＡＰＤ技术研究库内美洲黑杨无性系ＤＮＡ多态性,结果表明本库美洲黑杨ＤＮＡ多态率为８６％,再次证明本库美洲黑杨遗传多样性高,可为我国杨树改良提供丰富的育种材料。     

有益菌根真菌及其互作对带叶兜兰试管苗生理生长的影响

为探讨菌根真菌的互作效应,筛选协同互作模式组合,对从野生兜兰新鲜营养根中分离、筛选出的3种菌根真菌（PF02、PF06和PF07）,采用单菌接种、双菌混合接种和三菌混合菌种等7种接种方式,开展菌−苗共生培养,筛选出优势菌株PF02和PF07,揭示在实验室环境下接菌方式与接种效应之间的相互联系,分析菌根真菌互作对带叶兜兰植株生长和生理的影响。结果表明：显示菌根真菌互作对宿主植株的生物量、保护酶活性及叶绿素总量具有较好的正向效应。3种双菌混合接种方式PF02−PF06、PF02−PF07及PF06−PF07均能有效促进带叶兜兰生物量增长,尤其是PF02−PF06接种方式,抗氧化酶POD、超氧化物歧化酶SOD、过氧化氢酶CAT活性及叶绿素总量提高显著。选择优化的接种方式,以及能够协同促进、达到共生平衡的最佳接种方式组合,最大限度发挥菌根对宿主植物的益生作用。     

猪脐静脉血管内皮细胞的分离及体外培养研究

为建立一种操作简单并且能获得大量猪血管内皮细胞的分离培养方法,本研究采集新生健康长白猪脐带,取其脐静脉血管,灌注0.1%的Ⅰ型胶原酶,于37℃水浴消化10 min后收集内皮细胞,培养于含20%胎牛血清的M199培养基中,长满单层后用胰酶消化传代培养。并对培养的细胞形态学、相关抗原和其他特征以及对病毒的易感性进行鉴定。结果表明,Ⅰ型胶原酶消化后可获得大量内皮细胞,细胞培养后的形态学观察显示细胞贴壁生长良好,呈典型的铺路石状排列;第Ⅷ因子相关抗原和细胞摄取乙酰化低密度脂蛋白均呈阳性,内皮细胞比率可高达100%。传3代后的生长曲线显示细胞传代后延迟期小于1 d,对数生长期约3 d,第5 d进入平台期,第7 d开始脱落死亡。病毒感染试验表明所获得的内皮细胞对猪瘟病毒高度易感,病毒生长与常用的PK-15没有区别。上述研究结果表明本研究建立的血管内皮细胞分离培养方法简便有效,为大量制备原代血管内皮细胞提供了可靠的方法。     

光唇鱼网箱养殖试验

网箱单养、混养光唇鱼的试验结果表明,在25m2网箱中,单养光唇鱼以200～220尾/m2的放养密度比较合适,单位面积产量可达12.7～12.9 kg/m2;瓯江彩鲤网箱中混养光唇鱼,在瓯江彩鲤保持180尾/m2的放养密度不变的情况下,混养20尾/m2的光唇鱼,其效果比单养瓯江彩鲤更好,单位面积产量可达103.2kg/m2.     

Application of Principal Component Analysis on Workable Competition Evaluation

The effective competition is an effective organizational form of the industry in modem market economy. It is a form accompanied with large - scale production and vigor competition. The analysis of effective competition has been the focus that people have paid close attention to it very much all the time. It is a basis of industrial policy, on the basis of the former research, the aufhors bring out 9 indexes according to the actual situation of our country, then take automobile trade as an example, we analyze the automobile trade with principal component.     

可控硅移相单异步电动机的研究

由于单相异步电动机本身产生的磁场是脉振磁场,没有起动转矩,因此需要用一些辅助方法使其产生旋转磁场而得到起动转矩。以往采用的方法有:电容移相、电阻移相、罩极移相等辅助起动方法。本文介绍一种用双向可控硅移相的单相异步电动机起动方法,并用富里叶级数及双旋转磁场理论对系统中单相异步电动机输出转矩M随可控硅控制角α变化的规律进行了分析,求出了输出转矩M随α变化的函数关系,给出了出现最大转矩时的控制角α_M。最后,用实测值和理论值进行了比较,对理论值和实测值间的误差原因进行了分析。     

Ultrastructural pathogenesis of the PRRS virus

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) was first isolated in swine alveolar macrophages (SAMs) and has subsequently been reported to replicate in other cell lines. Entry of the virus inside the cell takes place by receptor-mediated endocytosis. Following the entry of the virus into the cell, several not completely understood changes take place. PRRSV has been reported to be an apoptotic-inductor virus both in vivo and in vitro. Interestingly, it has been suggested that PRRSV-induced apoptosis occurs in cells other than those in which PRRSV replicates by a bystander mechanism. In this paper the ultrastructural pathogenesis of PRRSV will be reviewed.     

Pathologic findings and association of Mycobacterium bovis infection with the bovine NRAMP1 gene in cattle from herds with naturally occurring tuberculosis

OBJECTIVE: To determine necropsy and Mycobacterium bovis culture results in cattle from herds with tuberculosis, the role of the bovine NRAMP1 gene in resistance and susceptibility to infection with M bovis, and the association between magnitude of the tuberculous lesions and various types of M bovis isolates. ANIMALS: 61 cattle from herds with tuberculosis in Texas and Mexico. PROCEDURE: 61 cattle were evaluated by necropsy; 59 had positive and 2 had negative caudal fold tuberculin intradermal test (CFT) results. Thirty-three cattle with positive CFT results were genotyped to evaluate polymorphism of the 3' untranslated region of the bovine NRAMP1 gene, using single-stranded conformational analysis, 9 were resistant to M bovis with no tuberculous lesions and negative M bovis culture results, and 24 were susceptible with tuberculous lesions and positive M bovis culture results. Isolates of M bovis were analyzed by restriction fragment length polymorphism (RFLP) on the basis of IS6110 sequences and direct-repeat fingerprinting patterns. RESULTS: 21 (35.6%; 21/59) cattle with positive CFT results had tuberculous lesions or positive culture results; in addition, 1 of 2 cattle with negative CFT results had tuberculous lesions and positive culture results. Tuberculous lesions were most common in the thorax (35/63; 55.5%) and lymphoid tissues of the head (10/63; 15.9%). Tuberculous lesions varied from 1 to 11/animal; 8 of 21 (38.1%) had solitary lesions. Associations were not found between resistance or susceptibility to infection with M bovis and polymorphism in the NRAMP1 gene or between the magnitude of the lesions and various RFLP types of M bovis isolates. CONCLUSIONS AND CLINICAL RELEVANCE: The NRAMP1 gene does not determine resistance and susceptibility to infection with M bovis in cattle.     

对妊娠母猪追加n-3类脂肪酸对其子宫脂肪酸含量的影响

用2个试验来评估母猪饲料中追加脂肪酸对母猪子宫脂肪酸构成的影响。在试验1中,组1为对照组(基础日粮),组2为亚麻组(基础日粮添加3.75%的亚麻),组3为保护性脂肪酸组(PFA)含有n-3多不饱和脂肪酸(PUFA)。当母猪170日龄时,对其进行配种使其怀孕。在妊娠40～43d将组1的7头、组2的5头和组3的8头妊娠母猪屠宰。结果表明,相比对照组,组2有增加胎儿二十碳五烯酸(EPA:C20:5n-3)的趋势(P=0.055);组3中胎儿的二十二碳六烯酸(DHA:C22:6n-3)含量显著增加;组2和组3中胎儿的绒毛尿囊膜中的DHA含量有显著增加趋势;在组2中的母猪子宫内膜中,EPA和二十二碳五烯酸(DPA)含量和对照组差异显著(P0.001;P0.05),组3的DHA含量也显著增加(P0.001)。总体来看,亚麻组(组2)和保护脂肪酸组(组3)都选择性地使得母猪子宫内膜和绒毛尿囊膜中的EPA和DHA含量有所增加。在试验2中,将含有PFA(1.5%)的170日龄的母猪日粮作为试验组(n=13/每组)日粮,对照组日粮为玉米-豆粕型。30d后采集其血液样本,当母猪205日龄时对其进行人工配种。妊娠11～19d采集其胎儿和母体子宫样本。血液样本数据显示,PFA日粮组的血浆中EPA和DHA含量都有所增加,且试验组的子宫内膜的EPA含量和对照组差异显著(P0.005);另外试验组胚胎外其他组织的EPA和DHA含量也成倍增加;PFA日粮组的19d胎儿的DHA含量较对照组也有所增加(P0.05)。     

我国出口观赏鸟血液原虫的调查与分析

通过显微镜检查对来源于全国各地的37种出口观赏鸟进行了血液原虫调查。共检查血样1144份，结果为：血液原虫的检出率为2．1％，主要是血变原虫和／或疟原虫。感染季节以秋冬两季为主，易感鸟品种主要是白腰文鸟、红喉歌鸲、蓝歌鸲、暗绿绣眼鸟、豆冠、白燕、情侣鹦鹉。