催乳保康散对奶牛隐性乳房炎的防治研究

我国奶牛乳房炎尤其是隐性乳房炎的发病率很高，一般隐性乳房炎的发病率达到20％～40％以上，甚至高达50％～80％。笔者根据传统的中兽医理论和最新的中草药研究成果，以及奶牛乳房炎的发病规律，以源自天然的毒副作用小、无残留、不易产生耐药性的中草药为原料，制成集增乳、保健、安全为一体的复合型绿色饲料添加剂催乳保康散，可以显著提高奶牛的产乳性能，为研究本饲料添加剂对奶牛隐性乳房炎的防治效果，进行了本试验。     

转译过程中小RNA病毒与宿主细胞的相互作用

小RNA病毒的转译机制不同于真核细胞mRNA的转译机制,该类病毒是借助于内部核糖体进入位点来启动内部翻译过程的,同时还伴随着宿主细胞蛋白质合成的抑制过程.在此过程中,小RNA病毒与宿主细胞间发生了一系列复杂的相互作用和信息交流,本文对该领域的研究进展作了综述.     

猪传染性胃肠炎病毒南京株纤突S蛋白基因的克隆与序列分析

参考 Genbank收录的 TGEV- Miller株的基因序列 ,自行设计合成 1对引物 (TGEVP5 /P6 ) ,对不同代次的 TGEV疫苗弱毒 STC3及种毒 、种毒 进行了 RT- PCR扩增 ,产物经琼脂糖凝胶电泳分析 ,均出现 1条大约 12 6 2 bp的目的条带 ,经 Eco R 酶切 ,都产生了 871bp和391bp左右的两个片段 ,与预期大小相符。将种毒 RT- PCR扩增目的条带回收纯化后克隆入PMD18- T载体中 ,转化宿主菌 DH5 α,挑选阳性克隆 (命名为 PTs) ,提取重组质粒 ,用 Hpa 、Eco R 对重组质粒进行酶切鉴定以及 PCR扩增 ,然后进行序列测定 ,并进行了序列分析 ,证实与国外标准毒株 Miller、Fs772 / 70、Purdue、TO14等有较高的同源性     

抗霉菌素“120”与杀灭菊酯混用防治效果试验

抗霉菌素“120”是一种新的农用抗菌素,防治农作物上多种病害都有效。实验室试验证明;本剂与杀灭菌酯混用,对防治病害和虫害的效果没有不良影响,因此可以减少打药次数,有一定经济意义。     

香菇出口生产技术综述

香菇无公害生产全程技术监控，包括优化产地生态环境栽培原材料把关，精选优良菌种，菌袋生产规范化，出菇科学管理，控制农药使用，产品标准化等七个方面。     

Effect of cGMP on voltage-gated potassium channel in pulmonary artery smooth muscle cells from rats exposed to chronic hypoxia

AIM: To investigate the effect of cGMP on voltage-gated potassium channel in pulmonary artery smooth muscle cells (PASMCs) from rats exposed to chronic hypoxia. METHODS: (1) Wistar rats were randomly divided into control group (group A) and chronic hypoxia group (group B). Then group B received hypoxia 8 hours per day for 4 consecutive weeks. (2) Single PASMC was obtained via acute enzyme separation method. (3) Conventional whole-cell patch clamp technique was used to record resting membrane potential (Em) and ion currents of voltage-gated potassium channel. The changes of ion currents of voltage-gated potassium channel before and after applying cGMP (1 mmol/L), an agonist of protein kinase G (PKG), and cGMP plus H-8 (1 mmol/L), an inhibitor of PKG were compared between two groups. RESULTS: The Em of group B were significantly lower than that of group A. The ion currents of voltage-gated potassium channel in group A and group B were all significantly inhibited by cGMP [control group: from (118.0±5.0) pA/pF to (89.9±16.5) pA/pF, n=6, P＜0.05;chronic hypoxia group: from (81.0±5.0) pA/pF to (56.8±9.1) pA/pF, n=6, P＜0.05]and these effects were reversed by H-8 [control group: from (119.2±10.3) pA/pF to (117.8±9.1) pA/pF, n=6, P＞0.05;chronic hypoxia group: from (96.8±6.2)　pA/pF to (98.0±2.2)　pA/pF, n=6, P＞0.05]. CONCLUSIONS: The currents of voltage-gated potassium channel was inhibited by chronic hypoxic. The inhibitory effect of cGMP on currents of voltage-gated potassium channel in PASMCs from both normal and chronic hypoxic rats may be probably through the phosphorylation of voltage-gated potassium channel.     

IL-12 induces T lymphocytes apoptosis and influences the expression and signal transduction of Fas/FasL

AIM: IL-12 acts upon Tlymphocytes and activates its receptor complexes of β1/β2,and so IL-12 can regulate TH1/TH2 balance. Our study is aimed at IL-12-inducing apoptosis of Tcells and expression and signal transduction of Fas/FasLduring Tcell apoptosis. METHODS: The apoptosis of Tcells was detected by Annexin Vstaining cytometry and the expression of Fas/FasLunder different inhibitors were detected by semi-quantitative PCR. RESULTS: IL-12 induced the human leukemic Tcell line(TIB-152) and the human lymphoma Tcell line(HTB-176) and the normal human Tcells to undergo apoptosis. The FasLexpression at 6 hours after treatment with IL-12 increased apparently, and reached the max at 24 hours, and FasLexpression induced by IL-12 was inhibited by PKCinhibitor. But IL-12 did not influence Fas expression. CONCLUSIONS: IL-12 can induce Tcells to undergo apoptosis which is characterized by early membrane changes, the inducing effect is correlated with the concentration of IL-12 and the maturation of Tcells. FasLparticipates in the progression of Tcell apoptosis as a apoptosis mediator, and the effect of IL-12 on FasLexpression may be related with PKCpathway.     

检测猪流行性腹泻病毒的R-PCR方法的建立

根据猪流行性腹泻病毒 (PEDV)的N基因自行设计和合成了一对可扩增长度为 641bp目的片段的引物 ,成功地建立了检测的猪流行性腹泻病毒的RT PCR方法。对猪轮状病毒 (PRV)、猪传染性胃肠炎病毒 (TGEV)的RT PCR检测结果均呈阴性。对PEDV JS株的RT PCR产物的序列分析表明 ,与CV777株的同源性为 97 3 %。     

表达H3N2亚型猪流感病毒HA基因重组伪狂犬病病毒的构建

将SV40启动子控制下的LacZ基因表达盒和CMV启动子控制下的H3N2亚型猪流感病毒（SIV H3N2）的HA基因插入到伪狂犬病病毒（PRV）通用转移载体pBdTK-Uni中，获得转移载体pLTK-HA。将该载体与PRV Bartha-K61株基因组DNA通过脂质体法共转染Vero细胞，经过10代蓝斑筛选、纯化和PCR鉴定获得了一株插入SIV HA基因的重组伪狂犬病病毒，命名为rPRV-HA。Western blotting和间接免疫荧光试验证实HA基因在重组病毒感染的细胞中获得了表达。用不同的细胞（PK-15、IBRS-2、Vero和鸡胚成纤维细胞）对该重组病毒与亲本病毒的增殖滴度和致细胞病变进行比较，未见显著差异，对第30代重组病毒的HA基因进行序列分析，表明该重组病毒遗传性状稳定。     

水分条件对巴音布鲁克高寒湿地CO_2排放的影响

在新疆天山中部巴音布鲁克天鹅湖高寒湿地,以苔草(Carex tristachya)为主要建群种的样地为研究对象,利用英国PP-systems公司生产的便携式土壤呼吸测定系统(CIRAS-2-SRC)研究了不同地表水分条件对天鹅湖高寒湿地夏季土壤CO2排放的影响。结果表明,1)湿润区的生物量大于干燥区;干燥区土壤CO2排放高于湿润区,干燥区土壤CO2排放日变化曲线为单峰曲线,CO2排放最高点出现在当地14:00-16:00,最高值为1.185 0g CO2·m-2·h-1;湿润区土壤CO2排放日变化曲线为双峰曲线,两个峰值分别出现在12:00和16:00,最高值为1.024 0g CO2·m-2·h-1。2)不同水分条件下生物量中凋落物含量影响土壤CO2排放。土壤温度是CO2排放的主要限制因子,且地表干燥区CO2排放与土壤温度的相关性更显著(P0.01)。土壤湿度与CO2排放相关性不显著(P湿润区=0.997,P干燥区=0.409)。