Perinatal hypoxia induces subsequent retinal degeneration in the offspring of ovoviviparous fish, Xiphophorous maculates

OBJECTIVE: This experiment evaluated the perinatal hypoxic effect on the retina of offspring of the ovoviviparous fish. ANIMAL STUDIED: The ovoviviparous fish Xiphophorous maculates was used for the experiment. PROCEDURE: The mothers were kept in a hypoxic environment of 3.5% oxygen for 6 h, starting 30 h before hatching. Subsequently, the retinae of the offspring were fixed, sectioned at 6 microm and evaluated microscopically from the age of 1 to 35 days. RESULTS: Degeneration of the outer nuclear layer of the retina was noted on the 3rd day and severe retinal degeneration was observed on the 35th day. Immunocytochemistry confirmed apoptosis by TUNEL reaction. There was no difference in neovascularization, as revealed by vascular endothelial growth factor, between controls (group 1) and hypoxic fish (group 2). CONCLUSIONS: Perinatal hypoxia could have long-lasting effects on the central nervous system in some species.     

在语文教学中如何培养学生的“创新精神”？

创新是一个民族进步的灵魂,是国家兴旺发达的不竭动力源泉。因此,对创新人才的培养,应该从基础教育抓起。为了在语文教学中培养学生的“创新精神”,教师首先应转变观念,使自己成为有创新精神的人。其次为学生创设和谐、民主的学习氛围,让学生最大限度地发展创造性思维能力。应让学生丰富知识、夯实基础、拓展视野,为创新思维的发展打下坚实的基础。     

Effect of intermittent starvation on growth and some antioxidant indexes of Macrobrachium nipponense (De Haan)

The effect of different periods of starvation (0, 2, 4 and 8 days) followed by re-feeding on growth, feed utilization, oxygen consumption and some immune indexes [reactive oxygen intermediates (ROIs), activities of superoxide dismutase (SOD) and catalase (CAT)] was evaluated over an 18-day experimental period in shrimp Macrobrachium nipponense (De Haan) that had an initial body weight of 0.52 g. As a result of compensatory growth, indicated by an increase in specific growth rate (SGR), feeding rate (FR) and feed conversion efficiency (FCE) after re-feeding, final body weight of shrimp starved for 2 days (0.63 g) and 4 days (0.65 g) did not differ ( P >0.05) from the control group (0.64 g), with feed withholding for 8 days presenting a significant lower value (0.63 g). Oxygen consumption rate (OCR) decreased during the starvation period in all groups, followed by a gradual increase to a similar ( P >0.05) value than found in the control group (0.47 mg kg⁻¹ h⁻¹) at the end of the experiment. Although ROIs and the activity of SOD and CAT fluctuated during starvation in the feed-deprived groups, values at the termination of the experiment were comparable ( P >0.05) to those found for the control group.     

Effects of dietary carbohydrate level on growth and body composition of juvenile tilapia, Oreochromis niloticus×O. aureus

A growth trial was conducted to feed juvenile tilapia (initial weight, 9.1±0.1 g), Oreochromis niloticus×O. aureus, isonitrogenous diets for 8 weeks. Six diets were formulated containing 29% crude protein from casein and gelatin, 10% crude fat from soybean oil and refined soybean lecithin and varying levels of corn starch ranging from 6% to 46% at increments of 8%, with corresponding energy to protein (E/P) ratios of 35.6, 37.9, 40.2, 42.5, 44.8 and 47.1 kJ g^?1. Weight gain (WG), specific growth rate, feed efficiency ratio and protein efficiency ratio were significantly higher in fish fed diets with starch ≥22% (or E/P ratio ≥40.2 kJ g^?1) than in fish fed diets with 6% or 14% starch (or E/P ratio of 35.6 or 37.9 kJ g^?1). No further improvement was measured when dietary starch content increased beyond 22%. Body protein retention showed the same general pattern as WG, and was highest in fish fed the 22% starch diet. Body composition was significantly affected by dietary starch level. Fish fed diets with starch ≥30% had significantly higher lipid content than fish fed diets with 6% or 14% starch. Ash content was negatively correlated with starch inclusion level, but moisture and protein contents did not show discernible trends among treatments. Results indicate that hybrid tilapia can utilize 46% dietary starch without growth retardation, while 22% starch in feed for juvenile tilapia containing 29% protein and 10% lipid, or an E/P ratio of 37.9 kJ g^?1 is optimal.     

Square Design of Mountain Cities: A Case Study of Shiyan Broadcasting Media Center Square

City squares are an important part of urban public spaces and play an important role in improving the quality of urban landscapes. For mountain cities, city squares with mountainous characteristics formed by its special geographical environment, which have more identifiability and uniqueness than that in plain cities, have greater influence on the promotion of city image. Their unique regional cultural characteristics and local customs and practices have shaped the totally different temperament of mountain cities. Based on the dialectical relationship between the square, the landscape and the culture, this paper took Shiyan Broadcasting Media Center Square for example to explore the ideas of landscape design in mountain cities.     

中密度纤维板工厂设计中的循环经济——减量化（Reduce）原则应用

文章叙述了在中密度纤维板产品工厂设计中尝试应用循环经济理论中的减量化原则,从提高原材料综合利用率,降低能源动力消耗量,清洁生产过程三方面入手,取得了较好的效果。     

果胶脱色技术研究进展

果胶脱色技术是影响果胶生产质量的重要因素,就果胶的呈色机理,活性炭脱色、醇氨溶液脱色、双氧水脱色、树脂脱色等方法进行了综述。