Scoring of the extent and intensity of carp (Cyprinus carpio) skin changes made by cormorants (Phalacrocorax carbo sinensis): relationship between morphometric and histological indices

Cormorants cause damage to fish in aquaculture not only by consuming large number of fish but also by wounding them. Quantification of the actual damage in fish induced by wounding is important for reliable estimations of the overall loss in aquaculture caused by cormorants. In this study, specimens of mirror and scaly carp (Cyprinus carpio L.) injured by attacks of great cormorant (Phalacrocorax carbo L.) were collected from the ??E?ka?? fish farm in Serbia. Surfaces of superficial and open subepithelial wounds (expressed as a percent of the total body surface), estimated by computer-assisted image analysis, had mean values 10.66?±?1.86 and 0.85?±?0.90, respectively. Histological evaluation of wounds was performed by two scoring systems: system proposed by Bernet et al. (J Fish Dis 22:25?C34, 1999) and modified system described by Wahli et al. (Aquaculture 225:371?C386, 2003). The most frequent histopathological changes were disturbed epidermal structure and surface cell morphology and increased number of club cells (on more than 75% of specimens); increased number of melanocytes and their distribution in different layers in dermis; and enlarged hypodermis (more than 80% of samples) and myolysis (50% of samples). Comparison of wounds?? surfaces (surface of superficial injures) and histopathological changes, estimated by two scoring systems, revealed significant positive correlation. Bernet??s scoring system showed higher correlation coefficients, when compared with the modified Wahli??s scoring system.     

Antioxidant activity of Filipendula hexapetala flowers

The antioxidant activity of the methanol extract of Filipendula hexapetala flowers was assessed by the assay for ferric-reducing antioxidant power (FRAP), the assay for DPPH free radical scavenging ability (DPPH) and the assay for the influence of lipid peroxidation in liposomes, induced by Fe(2+)/ascorbate system and measured by the TBA test (LP). The activity of the investigated extract in all test-systems was found to be significant. The principal constituent responsible for the observed effects was isolated and identified as spiraeoside.     

Nutritional management of gastrointestinal disease

The gastrointestinal (GI) tract is primarily responsible for acquiring and digesting food, absorbing nutrients and water, and expelling wastes from the body as feces. A proper diet and normally functioning GI tract are integral for the delivery of nutrients, prevention of nutrient deficiencies and malnutrition, repair of damaged intestinal epithelium, restoration of normal luminal bacterial populations, promotion of normal GI motility, and maintenance of normal immune functions (eg, both tolerance and protection from pathogens). The amount of food, its form, the frequency of feeding, and the composition of diet each have important effects on GI function and may be used to help ameliorate signs of GI disease. Although both nutrients and nonnutritional components of a diet are important to GI health, they also may cause or influence the development of GI pathology (eg, antibiotic responsive diarrhea, inflammatory bowel disease, dietary intolerance, or sensitivity and/or allergy). The appropriate diet may have a profound effect on intestinal recovery and successful management of chronic or severe GI disease.     

Effect of different management practices on water quality of intensive tilapia culture systems in Israel

Commercial intensive aquaculture systems werebuilt and are managed in a somewhat differentway in each farm. To evaluate the effects ofseveral management procedures on water qualityin intensive fish ponds, data from severallocations, times and culture conditions indifferent farms were collected and are hereinanalyzed through multivariate statistics. Water quality in the intensive ponds depends onthe water entering, the biological processeswithin, and the water leaving the ponds. Areservoir used as source and sink water supplied theintensive ponds with higher organic loadingthan clear source waters, and its phytoplanktoncontent affected nitrogen cycling within theintensive ponds. The systems with a reservoirhad better water quality in the intensive pondsthan those with only clean source water. Within the ponds (1) compared to paddle-wheelaeration, aeration by pure oxygen increasedoxygen concentration, improved nitrificationand promoted decomposition that reduced organicloading. (2) In concrete ponds accumulation oforganic matter and development of anerobicconditions on the pond bottom was higher thanin the slippery plastic-covered ponds. (3) Allintensive ponds provided good growthconditions, tilapia biomass having relativelysmall influence on water quality. Only inpaddle-wheel aerated ponds did increased tilapiabiomass increased inorganic nitrogen compoundsand soluble phosphorus through excretion, andreduce organic nitrogen through a moreefficient removal of food particles. Water leaving the ponds removes matteraffecting water quality within the pond. (1)Draining sediments accumulated on the bottomavoided development of anaerobic conditionswhere denitrification and phosphorus liberationcan occur. (2) Water exchange removed particleswith nitrifying bacteria and algae that absorbnutrients. A high water exchange rate may havea negative effect from the water quality pointof view and from the extra costs incurred inenergy and feeds washed out. The processes described occur simultaneouslythroughout the culture period and shape waterquality dynamics in the ponds. This researchcontributed to the understanding of howmanagement procedures affect the differentphases of water quality dynamics in real-scaletilapia commercial intensive systems.     

Assessment of anthocyanins in grape (Vitis vinifera L.) berries using a noninvasive chlorophyll fluorescence method

Anthocyanins (Anths) in grape (Vitis vinifera L.) berries harvested at véraison from Pinot Noir and Pinot Meunier cultivars were assessed nondestructively by measuring chlorophyll fluorescence (ChlF) excitation spectra. With increasing Anth content, less excitation light was transmitted to the deeper Chl layers, and thus the ChlF signal decreased proportionally. By applying Beer-Lambert's law, the logarithm of the ratio between the fluorescence excitation spectra (log FER) from a green and a red berry gave the in vivo absorption spectrum of Anths, which peaked at about 540 nm. Absolute quantitative nondestructive determination of Anths for each berry was obtained by the log FER calculated for two excitation wavelengths, 540 and 635 nm (absorbed and not-absorbed by Anths, respectively) of ChlF at 685 nm. Over a range of skin colors going from green to purple, the relationship between the log [ChlF(635)/ChlF(540)] and the Anth concentration of berry extracts was fairly well fitted (r 2 = 0.92) using a power function. Reflectance spectra on the same berry samples were also measured, and Anth reflectance indices, which were originally developed for apples and table grapes, were derived. The log FER Anth index was superior to the reflectance-ratio-based index, but was as good as the color index for red grapes (CIRG) calculated from the whole visible reflectance spectrum. The proposed log FER method, applied by means of suitable portable devices, may represent a new, rapid, and noninvasive tool for the assessment of grape phenolic maturity in vineyards.     

Antioxidant,Antimicrobial and Genotoxicity Screening of Hydro-alcoholic Extracts of Five Serbian <Emphasis Type="Italic">Equisetum</Emphasis> Species

The hydro-alcoholic extracts of five Equisetum species, E. arvense L., E. sylvaticum L., E. fluviatile L., E. palustre L. and E. telmateia Ehrh., growing-wild in Serbia were evaluated for their genotoxicity, antimicrobial activity, antioxidant capacity and the results related to the total phenol content and HPLC flavonoid profiles. The total phenol content was 92–349 μmol expressed as equivalents of chlorogenic acid per g of dried plant material. Main identified compounds were kaempferol-, quercetin- glycosides and caffeic acid derivatives. E. telmateia extract showed the greatest antioxidant capacity. Almost all tested microorganisms demonstrated some degree of sensitivity to the examined extracts. All tested extracts at 62.5 μg/ml showed higher incidence of micronucleus formation than in the control sample. The obtained data allowed mutual comparison of examined species and their assessment as possible sources of antioxidants, antimicrobials and/or genotoxic substances.     

Seasonal stratification in fish culture and irrigation reservoirs: potential dangers for fish culture

Dual-purpose reservoirs for field irrigation and fish culture are characterized by the simultaneous (a) increase in standing crop biomass and feeds during the fish culture period and (b) decrease of water level due to irrigation. This management practice affects stratification, which in turn affects water quality and may affect the fish stocked. To study the limnology of dual-purpose reservoirs, an extensive sampling programme including automatic continuous recording and manual water sampling in deep and shallow reservoirs was carried out. This paper describes limnological changes during the week in which a 3 month long seasonal stratification ended and fish kills occurred. Management implications of stratification are also discussed. The data presented are the first continuous records of this process in this particular type of water body.