Antioxidant activity of Filipendula hexapetala flowers

The antioxidant activity of the methanol extract of Filipendula hexapetala flowers was assessed by the assay for ferric-reducing antioxidant power (FRAP), the assay for DPPH free radical scavenging ability (DPPH) and the assay for the influence of lipid peroxidation in liposomes, induced by Fe(2+)/ascorbate system and measured by the TBA test (LP). The activity of the investigated extract in all test-systems was found to be significant. The principal constituent responsible for the observed effects was isolated and identified as spiraeoside.     

The study of coloration and antibacterial efficiency of corona activated dyed polyamide and polyester fabrics loaded with Ag nanoparticles

The aim of this study was to examine the influence of dyeing on antibacterial efficiency of corona activated polyamide and polyester fabrics loaded with colloidal Ag nanoparticles as well as the influence of the presence of Ag nanoparticles on the color change of dyed fabrics. C.I. Acid Green 25 and C.I. Disperse Blue 3 were used for dyeing of polyamide fabrics and C.I. disperse violet 8 for polyester fabrics. The color change of polyamide fabrics depends on the dye type, which was generally lower compared to polyester fabrics. Antibacterial efficiency of Ag loaded fabrics was tested against Gram-positive bacterium Staphylococcus aureus and Gram-negative bacterium Escherichia coli. Corona activated polyester and polyamide fabrics showed excellent antibacterial efficiency independently of order of dyeing and Ag loading. The morphology of fibers loaded with Ag nanoparticles was assessed by SEM and atomic absorption spectroscopy for elemental analysis.     

Novel Spray Dried Glycerol 2-Phosphate Cross-Linked Chitosan Microparticulate Vaginal Delivery System—Development,Characterization and Cytotoxicity Studies

Chitosan microparticulate delivery systems containing clotrimazole were prepared by a spray drying technique using glycerol 2-phosphate as an ion cross-linker. The impact of a cross-linking ratio on microparticle characteristics was evaluated. Drug-free and drug-loaded unmodified or ion cross-linked chitosan microparticles were examined for the in vitro cytotoxicity in VK2/E6E7 human vaginal epithelial cells. The presence of glycerol 2-phosphate influenced drug loading and encapsulation efficacy in chitosan microparticles. By increasing the cross-linking ratio, the microparticles with lower diameter, moisture content and smoother surface were observed. Mucoadhesive studies displayed that all formulations possessed mucoadhesive properties. The in vitro release profile of clotrimazole was found to alter considerably by changing the glycerol 2-phosphate/chitosan ratio. Results from cytotoxicity studies showed occurrence of apoptotic cells in the presence of chitosan and ion cross-linked chitosan microparticles, followed by a loss of membrane potential suggesting that cell death might go through the mitochondrial apoptotic pathway.     

Nutritional management of gastrointestinal disease

The gastrointestinal (GI) tract is primarily responsible for acquiring and digesting food, absorbing nutrients and water, and expelling wastes from the body as feces. A proper diet and normally functioning GI tract are integral for the delivery of nutrients, prevention of nutrient deficiencies and malnutrition, repair of damaged intestinal epithelium, restoration of normal luminal bacterial populations, promotion of normal GI motility, and maintenance of normal immune functions (eg, both tolerance and protection from pathogens). The amount of food, its form, the frequency of feeding, and the composition of diet each have important effects on GI function and may be used to help ameliorate signs of GI disease. Although both nutrients and nonnutritional components of a diet are important to GI health, they also may cause or influence the development of GI pathology (eg, antibiotic responsive diarrhea, inflammatory bowel disease, dietary intolerance, or sensitivity and/or allergy). The appropriate diet may have a profound effect on intestinal recovery and successful management of chronic or severe GI disease.     

Effect of different management practices on water quality of intensive tilapia culture systems in Israel

Commercial intensive aquaculture systems werebuilt and are managed in a somewhat differentway in each farm. To evaluate the effects ofseveral management procedures on water qualityin intensive fish ponds, data from severallocations, times and culture conditions indifferent farms were collected and are hereinanalyzed through multivariate statistics. Water quality in the intensive ponds depends onthe water entering, the biological processeswithin, and the water leaving the ponds. Areservoir used as source and sink water supplied theintensive ponds with higher organic loadingthan clear source waters, and its phytoplanktoncontent affected nitrogen cycling within theintensive ponds. The systems with a reservoirhad better water quality in the intensive pondsthan those with only clean source water. Within the ponds (1) compared to paddle-wheelaeration, aeration by pure oxygen increasedoxygen concentration, improved nitrificationand promoted decomposition that reduced organicloading. (2) In concrete ponds accumulation oforganic matter and development of anerobicconditions on the pond bottom was higher thanin the slippery plastic-covered ponds. (3) Allintensive ponds provided good growthconditions, tilapia biomass having relativelysmall influence on water quality. Only inpaddle-wheel aerated ponds did increased tilapiabiomass increased inorganic nitrogen compoundsand soluble phosphorus through excretion, andreduce organic nitrogen through a moreefficient removal of food particles. Water leaving the ponds removes matteraffecting water quality within the pond. (1)Draining sediments accumulated on the bottomavoided development of anaerobic conditionswhere denitrification and phosphorus liberationcan occur. (2) Water exchange removed particleswith nitrifying bacteria and algae that absorbnutrients. A high water exchange rate may havea negative effect from the water quality pointof view and from the extra costs incurred inenergy and feeds washed out. The processes described occur simultaneouslythroughout the culture period and shape waterquality dynamics in the ponds. This researchcontributed to the understanding of howmanagement procedures affect the differentphases of water quality dynamics in real-scaletilapia commercial intensive systems.     

Antimicrobial activity of Chenopodium botrys essential oil

Essential oil, isolated by hydrodistillation from Chenopodium botrys aerial parts, expressed significant bactericidal and fungicidal activity against selected strains of microorganisms.     

Development of sperm vitrification protocols for two endangered salmonid species: the Adriatic grayling, <Emphasis Type="Italic">Thymallus thymallus</Emphasis>, and the marble trout, <Emphasis Type="Italic">Salmo marmoratus</Emphasis>

Vitrification was applied to the sperm of two endangered fish species of So?a River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA). Fertilization trials were carried out to test the effectiveness of vitrification in the case of grayling. A protocol utilizing a glucose-based extender, 30% cryoprotectants (15% methanol + 15% propylene glycol), 1:1 dilution ratio, and droplets of 2 μl on a Cryotop as cooling device yielded the highest post-thaw motility values for both Adriatic grayling (7.5?±?6.5%) and marble trout (26.6?±?15.8%). Viable embryos were produced by fertilizing eggs with vitrified grayling sperm (hatching 13.1?±?11.7%, control hatching 73.9?±?10.4%). The vitrification protocol developed in this study can be utilized in the conservation efforts for the two species as an alternative to slow-rate freezing when working in field conditions or when specific equipment necessary for slow-rate freezing is not available.