不同投喂方式对革胡子鲶消化酶的影响

在（25±1）℃水温、自然光照条件下,设3种投喂方式饲养革胡子鲶（clarias leath-er）：第1种连续投喂整个试验期间不间断,第2和3种分别连续投喂3、7 d 后饥饿1 d,3种方式分别以R0（对照组）、R1/3、R1/7表示,试验周期共45 d。结果显示：R0、R1/3和R1/7组胃、后肠和肝脏中蛋白酶活性影响差异不显著,但前肠和中肠内蛋白酶活性影响存在差异(P<0.05)。R0、R1/3、R1/7组的胃、前肠、中肠、后肠和肝脏中淀粉酶影响不显著,R0、R1/3、R1/7的胃、前肠和肝脏内脂肪酶活性影响差异显著(P<0.05),而R0、R1/3、R1/7组中肠和后肠中脂肪酶活性影响差异不显著(P>0.05)。     

老年犬的养护

如果家里有一头老年犬,它一定陪伴你走过了好几个年头,在这么多年里它也许是你的精神寄托;有了它你的生活更快乐了,是它提高了你的生活品质。这个时候,它仍然在你的身旁,但它已经不像年轻时那样爱玩爱闹了,对事情的注意力、警惕性也有明显下降。这些都是不可避免的,你深爱的犬正在逐渐衰老。这个时候,它的需求正在变化,它对你的依赖越来越强,它需要你给它保持一个健康的身体和安逸的老年生沽。     

Inductive expression of the NOD1 signalling pathway in chickens infected with Salmonella pullorum

1. The aim of this study was to describe the role of Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) receptor signalling in chicken.

2. Tissue-specific expression analysis of NOD1, receptor-interacting serine-threonine kinase 2 (RIPK2), nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase 11 (MAPK11 or p38) by quantitative real-time PCR (qRT-PCR) revealed their wide distribution in various organs and tissues.

3. Salmonella pullorum infection activated NOD1 receptor signalling in vivo and in vitro, resulting in significant induction of downstream signalling molecules RIPK2, NF-κB/p65, MAPK11/p38 and the effector molecules IL-1b and IL-8.

4. Activation of NOD1 by its agonist bacterial γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP) in HD11 cells induced the adapter molecular RIPK2 and activated the NF-κB/p65 and MAPK11/p38 pathways, resulting in an increase in IL-8 but not IL-1β. Additionally, inhibition of NOD1 using NOD1-shRNA resulted in downregulation of RIPK2, MAPK11 and IL-8, while NF-κB/p65 and IL-1β were unaltered.

5. These results highlight the important role of NOD1 receptors in eliciting the innate immune response following pathogenic invasion in chicken.     

丰宁县森林食品资源现状及开发利用前景

随着科学技术的进步、人类生活水平的提高,天然绿色食品受到人们的青睐。森林经济植物种源丰富,其可食部分的营养价值及微量元素有的优于栽培植物,开发利用前景广阔。1989年,丰宁林业局同原北京林学院等单位联合,对丰宁森林木本植物资源进行了清查,摸清了种源分布,为合理?..     

中国河西走廊草地农业的基本格局和它的系统相悖─—草原退化的机理初探─—

河西走廊的草地资源位于西北荒漠的大背景中，由山地、荒漠和绿洲三个子系统构成，其中荒漠子系统居主导地位，具有先天的脆弱性。它们在人为干预下，在自然生态系统的农业化过程中，发生了山地一绿洲一荒漠组成的耦合农业系统。由于耦合不够完善，它所固有的相悖因素未能有效控制，形成涵有系列相悖因子群的系统相悖。主要表现为时间相悖，空间相悖和种间相悖。三者互相影响，但时间相悖起主导作用，使系统的生态危机加剧。它通常表现为草原退化。对草地农业生态系统的自由能的调控，保持自由能的积累和输出基本相当，是防止草原退化的根本出路。草原退化是草地对于草食动物压迫的对策系统和动物对于草地负荷行为的对策系统的综合反映。放牧程度可以作为草原退化过程的向量系统，但不是草原退化的本质。     

Pharmacokinetics and bioavailability of valnemulin in broiler chickens

Wang, R., Yuan, L.G., He, L.M., Zhu, L.X., Luo, X.Y., Zhang, C.Y., Yu, J.J., Fang, B.H., Liu, Y.H. Pharmacokinetics and bioavailability of valnemulin in broiler chickens. J. vet. Pharmacol. Therap. 34 , 247–251. The objective of this study was to investigate the pharmacokinetics and bioavailability of valnemulin in broiler chickens after intravenous (i.v.), intramuscular (i.m.) and oral administrations of 10 mg/kg body weight (bw). Plasma samples were analyzed by high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS). Pharmacokinetic characterization was performed by non‐compartmental analysis using WinNonlin program. After intravenous administration, distribution was wide with the volume of distribution based on terminal phase(V_z) of 4.27 ± 0.99 L /kg. Mean valnemulin t_1/2β(h), Cl_β(L /h /kg), V_ss (L /kg) and AUC_(0–∞)(μg·h /mL) values were 2.85, 0.99, 2.72 and 10.34, respectively. After intramuscular administration, valnemulin was rapidly absorbed with a C_max of 2.2 μg/mL achieved at 0.43 h (t_max), and the absolute bioavailability (F) was 88.81%; and for the oral route the same parameters were 0.66 ± 0.15 μg/mL, 1.54 ± 0.27 h and 74.42%. A multiple‐peak phenomenon was present after oral administration. The plasma profile of valnemulin exhibited a secondary peak during 2–6 h and a tertiary peak at 32 h. The favorable PK behavior, such as the wide distribution, slow elimination and acceptable bioavailability indicated that it is likely to be effective in chickens.     

DNA Methylation Pattern in Pronuclear-Stage Mouse Embryos: Effect of Oocyte Vitrification

This study was conducted to investigate the pattern of DNA methylation in pronuclearstage mouse embryos derived from vitrified-warmed oocytes.Mouse oocytes at metaphase Ⅱ (MⅡ) stage of meiosis were all...     

平行串叶法小蚕片叶立体一日一回育技术的研究

在小蚕片叶立体一日一回育的基础上,试验了平行串法小蚕片叶立体一日一回育,与立体一日二回育比,节约用桑３％,节约劳力３６％,张种产量提高８．５％,还阐明了该法的技术要点。     

黑龙江省东南部林区6种啮齿动物食性分析

为了准确掌握黑龙江省东南部地区对农林危害比较严重的啮齿动物的食性及食物量,以便更好地、有针对性地灭鼠,从而为林业部门预防鼠害提供科学依据,于2010年3月—2011年1月通过实地考察,对捕获的鼠进行解剖,并结合实验室喂养进行食物量测定。结果表明,种子和浆果是树栖型种类啮齿动物的主要食物,约占70%左右;而地栖型种类啮齿动物的食物存在明显的季节性和地域性差异。     

四季鹅产蛋前后腺垂体远侧部细胞的超微结构比较

对处于产蛋、停产和产前等不同时期的四季鹅腺垂体远侧部分泌促激素的６类细胞,即促生长激素细胞（α细胞）、催乳激素细胞（η细胞）、促卵泡形成激素细胞（β细胞）、促黄体生成激素细胞（γ细胞）、促甲状腺激素细胞（δ细胞）及嫌色细胞等的超微结构作了比较观察。结果表明,产蛋期嗜碱性β、γ和δ细胞呈旺盛的细胞结构象,细胞器结构发达,腺体功能活跃;停产期特征是η细胞稍增多,细胞器少,腺体功能处于相对静止;产前期仅α细胞功能活跃,嫌色细胞数量较多,此时腺体功能低下。