全文获取类型
收费全文 | 27512篇 |
免费 | 1379篇 |
国内免费 | 3317篇 |
专业分类
林业 | 3226篇 |
农学 | 4118篇 |
基础科学 | 2617篇 |
5099篇 | |
综合类 | 7960篇 |
农作物 | 1529篇 |
水产渔业 | 1060篇 |
畜牧兽医 | 3819篇 |
园艺 | 797篇 |
植物保护 | 1983篇 |
出版年
2024年 | 99篇 |
2023年 | 317篇 |
2022年 | 808篇 |
2021年 | 1120篇 |
2020年 | 1002篇 |
2019年 | 999篇 |
2018年 | 660篇 |
2017年 | 961篇 |
2016年 | 967篇 |
2015年 | 1326篇 |
2014年 | 1205篇 |
2013年 | 1430篇 |
2012年 | 1673篇 |
2011年 | 1842篇 |
2010年 | 1697篇 |
2009年 | 1617篇 |
2008年 | 1521篇 |
2007年 | 1668篇 |
2006年 | 1490篇 |
2005年 | 1399篇 |
2004年 | 673篇 |
2003年 | 540篇 |
2002年 | 481篇 |
2001年 | 511篇 |
2000年 | 604篇 |
1999年 | 759篇 |
1998年 | 642篇 |
1997年 | 603篇 |
1996年 | 529篇 |
1995年 | 544篇 |
1994年 | 437篇 |
1993年 | 413篇 |
1992年 | 349篇 |
1991年 | 305篇 |
1990年 | 257篇 |
1989年 | 204篇 |
1988年 | 198篇 |
1987年 | 115篇 |
1986年 | 70篇 |
1985年 | 29篇 |
1984年 | 28篇 |
1983年 | 32篇 |
1982年 | 33篇 |
1981年 | 14篇 |
1980年 | 14篇 |
1979年 | 7篇 |
1973年 | 2篇 |
1964年 | 4篇 |
1963年 | 3篇 |
1934年 | 2篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
102.
103.
雄性不育是水稻杂种优势利用的重要资源,对雄性不育现象的研究具有重要理论意义和实践价值。本研究以自然突变的水稻雄性不育突变体012S-3为试验材料,对其表型特征和花粉育性等进行调查,并构建遗传群体,利用分子标记对目的基因进行初步定位,然后应用基因组重测序技术对其进行精细定位。结果表明,012S-3是一个典型的无花粉普通型雄性不育材料,其不育性状受1对隐性核基因控制。初步定位分析目的基因与SSR标记RM6081存在连锁关系,其遗传距离约为34.4 c M;进一步的精细定位分析,找到3个候选基因:LOC_Os07g35880、LOC_Os07g35920和LOC_Os07g35940,其中LOC_Os07g35880和LOC_Os07g35940编码β-淀粉酶,属于水稻中新发现的花粉致死基因。该不育基因的成功定位为其进一步的分离克隆及其在水稻分子设计育种中的应用奠定了基础。 相似文献
104.
Xue-Yin Li Ling Tang Kai Hu Yang Liu Wei-Dan Jiang Jun Jiang Pei Wu Gang-Fu Chen Shu-Hong Li Sheng-Yao Kuang Lin Feng Xiao-Qiu Zhou 《Fish physiology and biochemistry》2014,40(3):659-671
The dietary lysine requirement of sub-adult grass carp (460 ± 1.5 g) was assessed by feeding diets supplemented with grade levels of lysine (6.6, 8.5, 10.8, 12.9, 15.0 and 16.7 g kg?1 diet) for 56 days. The test diets (28 % CP) contained fish meal, casein and gelatin as sources of intact protein, supplemented with crystalline amino acids. Weight gain (WG), feed intake and feed efficiency were significantly improved with increasing levels of lysine up to 12.9 g kg?1 diet and thereafter declined (P < 0.05). Quadratic regression analysis of WG at 95 % maximum response indicated lysine requirement was 10.9 g kg?1 diet. Activities of trypsin, chymotrypsin, lipase, Na+, K+-ATPase and alkaline phosphatase in intestine, creatine kinase activity in proximal and mid-intestine responded similar to WG (P < 0.05). In addition, lipid and protein oxidation decreased with increasing levels of lysine up to certain values and increased thereafter (P < 0.05); the anti-hydroxyl radical capacity, dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase (GST) activities and glutathione content were increased with increasing dietary lysine levels up to certain values in the detected tissues, except for hepatopancreatic GST. Requirement estimated on the basis of malondialdehyde content in intestine and hepatopancreas was 10.6 and 9.53 g lysine kg?1 diet, respectively. 相似文献
105.
Pengfang Zhu Zhendong Tian Zhichao Pan Xin Feng 《The Journal of Horticultural Science and Biotechnology》2018,93(5):466-473
Anthocyanins are responsible for the colour of many fruits, vegetables, flowers, and coloured-leaved trees. Ornamental kale (Brassica oleracea L. var. acephala DC) is widely cultivated for its colourful inner leaves. To investigate the relationship between the degree of colouration and anthocyanin distribution, content, and composition in ornamental kale, the authors studied the pigment characteristics of five cultivars with different coloured leaves (white, pink, red, purple, and purple-black). Microscopy observation, spectrophotometer, and high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of fresh inner leaves revealed that pink, red, and purple colourations were associated with high levels of anthocyanin, while purple-black was the result of the combination of anthocyanins and chlorophyll. In the coloured cultivars, anthocyanins were abundant mainly in the first and second cell layers below the epidermis in both the hypocotyls and inner leaves. No anthocyanin was found in the white-leaved phenotype cultivar. Anthocyanin content increased as leaf colour deepened from pink, red, to purple cultivars, which had little chlorophyll and carotenoid. The authors identified eight anthocyanins in the four coloured cultivars, including one non-acylated, four monoacylated, and three diacylated cyanidin glycosides. Cyanidin-3-(sinapoyl)(feruloyl)-diglucoside-5-glucoside was the most abundant anthocyanin in the four coloured cultivars followed by cyanidin-3-(sinapoyl)-diglucoside-5-glucoside. The analysis of anthocyanin accumulation characterisation provides important information on evaluating colouration patterns in coloured plants, and will be helpful for breeding desired leaf colours in ornamental kale. 相似文献
106.
107.
关于修订桃种质资源(Prunus persica)描述体系的建议 总被引:2,自引:2,他引:2
评价是种质资源工作的重要环节之一,是资源利用与共享的基础。目前,中国桃种质资源的评价描述体系一般是按照中国1990年编制的“桃种质描述符”进行。通过对国际植物遗传资源委员会1980年编制“桃描述系统″和中国“桃种质描述符”在评价的层次、评价内容、评价方法和评价标准的系统比较,以及2个标准在种质资源科研实践使用过程中存在的不足之处,结合中国桃种质资源工作的特点和国外资源的描述方法的新进展,提出中国桃种质资源描述系统的修改建议,为建立中国标准化、数字化的桃种质资源描述系统奠定基础。 相似文献
108.
一种水产养殖用微生物制剂的研究 总被引:1,自引:0,他引:1
利用枯草芽孢杆菌、干酪乳杆菌、沼泽红假单胞菌和啤酒酵母4种菌株配制了一种复合微生物制剂,试验结果表明,该制剂除含有丰富的营养成分外,在水产养殖中还能起到净水剂的作用,它能增加水中的溶解氧,显著降低氨氮、亚硝态氮、硫化物等不利于水产养殖的污染成分。此微生物制剂在水产养殖中将能发挥营养、保健及净化水质的功效。 相似文献
109.
110.
H. Hu A. Barker T. Harcourt‐Brown N. Jeffery 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(6):1456-1463
Intracranial neoplasia is commonly diagnosed in dogs and can be treated by a variety of methods, but formal comparisons of treatment efficacy are currently unavailable. This review was undertaken to summarize the current state of knowledge regarding outcome after the treatment of intracranial masses in dogs, with the aim of defining optimal recommendations for owners. This review summarizes data from 794 cases in 22 previously published reports and follows PRISMA guidelines for systematic review. A Pubmed search was used to identify suitable articles. These then were analyzed for quality and interstudy variability of inclusion and exclusion criteria and the outcome data extracted for summary in graphs and tables. There was a high degree of heterogeneity among studies with respect to inclusion and exclusion criteria, definition of survival periods, and cases lost to follow‐up making comparisons among modalities troublesome. There is a need for standardized design and reporting of outcomes of treatment for brain tumors in dogs. The available data do not support lomustine as an effective treatment, but also do not show a clear difference in outcome between radiotherapy and surgery for those cases in which the choice is available. 相似文献