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81.
Yongtao Li Wei Zou Guangmin Jia Jianjiang Ke Jiping Zhu Xian Lin Hongbo Zhou Meilin Jin 《Veterinary research》2013,44(1):41
Since the emergence of the 2009 pandemic (H1N1) virus (2009/H1N1) in April 2009, cases of transmission from humans to pigs have been reported frequently. In our previous studies, four 2009/H1N1 variants were isolated from pigs. To better understand the phenotypic differences of the pig isolates compared with the human isolate, in this study mice were inoculated intranasally with different 2009/H1N1 viruses, and monitored for morbidity, mortality, and viral replication, cytokine production and pathological changes in the lungs. The results show that all isolates show effective replication in lungs, but varying in their ability to cause morbidity. In particular, the strains of A/swine/Nanchang/3/2010 (H1N1) and A/swine/Nanchang/F9/2010 (H1N1) show the greatest virulence with a persisting replication in lungs and high lethality for mice, compared with the human isolate A/Liaoning /14/2009 (H1N1), which shows low virulence in mice. Furthermore, the lethal strains could induce more severe lung pathological changes and higher production of cytokines than that of other strains at an early stage. Amino acid sequence analysis illustrates prominent differences in viral surface glycoproteins and polymerase subunits between pig isolates and human strains that might correlate with their phenotypic differences. These studies demonstrate that the 2009/H1N1 pig isolates exhibit heterogeneous infectivity and pathogencity in mice, and some strains possess an enhanced pathogenicity compared with the human isolate. 相似文献
82.
[目的 ]构建重大动物疫病暴发流行预警指标体系。[方法 ]以禽流感和口蹄疫为例,采用文献调研法和德菲尔专家咨询法中的专家会议法初拟重大动物疫病预警体系的框架和指标;应用德菲尔专家咨询法评价预警指标的重要性、可操作性;应用组合权重法比较各指标综合影响的大小,并将指标分类。[结果 ]筛选出禽流感和口蹄疫预警预报一级指标6项(易感动物、饲养管理和免疫情况等)以及二级指标14项(免疫效果、环境消毒、温度等),并将指标分为三类(关键指标、重要指标和一般指标)。[结论 ]初步构建了重大动物疫病暴发流行预警指标体系。 相似文献
83.
这是一起在云南警方组织开展的“天网三号”禁毒专项行动中多警种协作、群众踊跃参与、警犬投入侦破的典型案例:2006年3月12日,当3名嫌疑人面对从两公里外查获的3背包沉甸甸的毒品海洛因及其他赃物拒不认罪、案件侦查陷入僵局时,刑侦犬“安普贵”奉命出击,把毒品和毒贩锁定在一 相似文献
84.
我国奶牛养殖小区发展中存在的问题及对策 总被引:1,自引:1,他引:1
奶牛养殖小区是近些年来我国奶牛饲养业出现的一种新的饲养组织模式;本文总结了在其快速发展过程中暴露出的一些问题,如设计建设不科学、不规范,存在很大的疫病传播风险,管理和技术水平较低等,指出在创建奶牛养殖小区之前一定要进行科学论证及标准化建设、要加强奶牛小区疾病的防疫工作、实行科学规范的管理等。 相似文献
85.
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87.
提高蛋白质和赖氨酸水平对初产哺乳母猪繁殖性能的影响 总被引:1,自引:2,他引:1
研究选用16头健康的纯种约克夏初产哺乳母猪,采用2×2因子设计,分为4个处理,每个处理4个重复,研究2种蛋白水平下添加Lys对哺乳母猪繁殖性能的影响。结果表明:在同水平的蛋白质含量时,添加Lys能显著增加哺乳母猪采食量,提高其繁殖性能;无论是否添加Lys,高蛋白水平的日粮都优于低蛋白。最合适的日粮组合方式为高蛋白日粮中添加Lys。 相似文献
88.
Genetic differences in oestrous signs and oestrogen metabolism‐related genes between Chinese Mi and European Landrace‐Large White pigs 下载免费PDF全文
Oestrous signs affect timely mating and reproductive efficiency in swine breeding herds. To study the genetic difference of oestrous signs between Chinese and European pigs, 100 Landrace‐Large White (LLW) cross gilts and 50 Chinese Mi gilts were assessed for oestrous signs and the concentrations of serum estradiol‐17β and progesterone were determined. The genotype of 39 single nucleotide polymorphisms (SNPs) in 11 oestrogen metabolism and function‐related genes was determined by Sequenom iPLEX platform. Compared with LLW gilts, Mi gilts had longer time of standing reflex (p < .001), higher scores of vulva reddening (p = .001) and greater serum estradiol‐17β concentration (p < .01). Gilts with greater serum estradiol‐17β concentrations also had greater (p < .05) scores for oestrous signs. Genetic polymorphisms of nine genes in oestrogen metabolism pathways had significant differences (p < .05) between LLW and Mi gilts. There were three and six haploblocks of SNPs in LLW and Mi, respectively. Compared with LLW, the distribution of haplotypes was more centralized in Mi pigs. Genetic polymorphisms of oestrogen metabolism‐related genes have considerable differences between Chinese Mi and European LLW pigs. Because of the important roles of oestrogen during the oestrus, some genes of oestrogen metabolism pathway could be considered as candidate genes for oestrous signs. 相似文献
89.
Zuoyong Zhou Kui Nie Cheng Tang Rongqiong Zhou Ze Zhang 《Research in veterinary science》2010,89(2):262-265
To identify the species within the genus Anaplasma circulating among ruminants in the Southwest of China, we performed the phylogenetic analysis of the 16S rRNA gene of two Anaplasma isolates from cattle and seven from goats. The two sequences obtained from cattle strains belonged to the A. marginale cluster, whereas the other seven sequences from caprine strains formed two Anaplasma spp. clusters, which diverged earlier than the clusters of A. marginale, A. centrale and A. ovis. These results indicate that there are at least two Anaplasma species circulating among ruminants in Southwestern China. 相似文献
90.
本文研究了3种稀释液在0~5℃下保存火鸡精液的效果及稀释液pH值、稀释比例和卵黄浓度对精子存活力的影响。原精及稀释精液Ⅰ、Ⅱ、Ⅲ不保存时,受精率分别为71.7%,64.03%,63.29%和63.77%(P<0.05);在0~5℃下保存48h后,原精完全丧失受精率(0%),而稀释精液Ⅰ、Ⅱ、Ⅲ的受精率分别为52.40%,45.30%和20.33%(P<0.01)。火鸡精液在0~5℃保存过程中,pH值降低,原精比稀释精液降低程度大。在本研究条件下,pH6.5和1:3稀释最适合于火鸡精子的存活,稀释液中添加5%或10%卵黄可提高火鸡精子的存活力。 相似文献