右美托咪定抑制NOX4缓解急性应激致大鼠肾损伤

本研究拟探索右美托咪定（dexmedetomidine,DEX）对大鼠急性应激致肾损伤的保护作用,并从氧化应激的角度探索DEX对大鼠肾的保护通路。本研究使用了急性束缚应激模型,其中,大鼠被迫游泳15 min,并束缚3 h。本试验采用生化检测、组织病理学切片观察以评估肾功能,然后测定了氧化应激以及氧化应激的相关通路蛋白。旷场试验证实成功建立了急性应激模型。急性应激引起的肾损伤增加了NOX4,降低了Nrf2/HO-1/NQO1表达水平。DEX可降低NOX4表达,同时升高Nrf2/HO-1/NQO1的表达水平。DEX治疗组与急性应激组相比的肾生化结果明显恢复正常,病理切片观察损伤显著降低。试验结果表明,DEX治疗急性应激可影响NOX4/Nrf2/HO-1/NQO1信号通路,并抑制氧化应激。因此,DEX对急性应激引起的肾损伤具有保护作用,并在应激综合征中具有潜在的临床应用。     

龙牙百合的植株再生与遗传转化

以龙牙百合鳞片叶切块为外植体，通过多种培养基的比较试验，得出MS 2，4-D 2mg／L 6-BA 0．2mg／L是诱导愈伤的最佳培养基，MS 6-BA 1mg／L NAA 0．05mg／L是不定芽诱导及伸长的适宜培养基。MS N_AA2mg／L是生根的最佳培养基。本试验已建立了适用于龙牙百合遗传转化的快速高频再生系统。用携带有几丁质酶基因和β—1、3葡聚糖酶基因的工程菌，通过农杆菌介导法和基因枪转化法转化龙牙百合，经PCR和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较，发现农杆菌介导法的转化率为16．7％，基因枪法的转化率为50％，因此可能基因枪转化法更适于龙牙百合的遗传转化。     

Influence of Steam Turbine System on Low Frequency Oscillation

Low frequency oscillation of electric power system is a problem which electric workers always care about. The influences of prime mover characteristics were ignored in the past research. The paper introduces the models of steam turbine system and each part of electric power system, and calculates the corresponding eigenvalues, then analyzes the influences of steam turbine system on low frequency oscillation.     

Analysis of thermal stress in a water pipe of blast furnace copper stave

To solve the general problem that copper staves experience water pipe failure in blast furnace,the effects of constraints on the thermal stress distribution of copper stave water pipe are investigated by thermal-structural coupling analysis.In this study,a mathematical model of thermal stress of copper stave is established to analyze the thermal deformation and stress distribution of water pipe under subject to different types of constraints.The results show that the thermal stress on the root of water pipe is larger than others as the terminal of water pipe is free or being fixed.But the equivalent stress on the root of water pipe is just 45 MPa as the terminal of water pipe is free,which is lower than the yield strength of pure copper.The equivalent stress on the root of water pipe is about 272 MPa as the terminal of water pipe is being fixed,and exceeds the yield strength of pure copper.The water pipes should be flexible.The maximum equivalent stress is about 600 MPa as the water pipe is subjected to the constraint of shell,which is far beyond the yield strength of pure copper.The maximum displacement is located at the end of water pipe and is about 1.73 mm.Then the diameter of the hole should be 120 mm with taking installation error into consideration.     

Dynamics of Actin Polymerization

The process of actin polymerization and depolymerization have some relation with its function,the activation of such process is compatible to its functions. The polymerization progress is divided into four steps: The first step is the activation of the monomer actin. The nucleation of actin nucleus forms in the second step which limits the polymerization speed. The elongation of the F-actin filaments happens in the following step. In the last step, a steady-state is attained, the length of the filament does not change because the polymerizing rate in the barbed end is the same with the depolymerizing rate in the pointed end. Some factors which affect the polymerizing process, such as nucleotide, actin related proteins are discussed. The aim is to know more about how to assemble the complicated system with biomacromolecule and what to function.     

Adaptive Intrusion Detection System Based on Agent and Data Mining

Intrusion detection system is an essential component of network security protection mechanisms. Most intrusion detection system can not adapt the variation of network environment. Aiming at this problem, an adaptive strategy that composed of condition space and strategy space is proposed. The condition space describes the network environment and the strategy space describes the strategy. There is an exclusive strategy corresponds to a certain environment state in condition space. On the base of the adaptive strategy, an adaptive intrusion detection system based on agent and data mining is designed. The simulation experiments indicate that the adaptive strategy is effective.     

Water model study on flow optimization of five streams L-type tundish

In this paper, the water model method is used to optimize the flow behavior of asymmetrical five streams L-type tundish in a steelmaking plant. The results show that the optimization plan is set up with turbulence suppressor T+ vice guide wall D2+ main guide wall with three holes (D1a) which consists of top two holes with 25° inclination and one hole with 30° inclination below. With this plan, the time which the colorants reach to the outlets increases, the difference of response time in all outlets decreases, the peak concentration of the RTD reduces, the existence time of high concentration liquid steel extends, and the ratio of dead zone in each streams reduces.