Alfalfa Phytoestrogen Content: Impact of Plant Maturity and Herbage Components

Legumes contain a range of non‐nutritional phytochemicals that may have health‐promoting effects in humans. In this study, we determined the concentrations of four phytoestrogens (coumestrol, apigenin, luteolin and quercetin) in field‐grown alfalfa (Medicago sativa L.). Differences between plants of different stages of maturity, between plant parts, and different canopy segments were assessed. The concentration of individual phytoestrogen in whole herbage varied between 15 and 225 μg g^?1 dry matter (DM) and was strongly affected by stage of maturity. Coumestrol and apigenin concentrations were highest at early vegetative stages, luteolin and quercetin at early vegetative and late flowering stages. All phytoestrogens were found in lowest concentrations at the early flowering stage (average 68 μg g^?1 DM); stage at which alfalfa is usually harvested when used as a forage source for animals. At vegetative stages, apigenin was the predominant phytoestrogen in herbage followed by coumestrol, the reverse being observed upon initiation of flowering; luteolin and quercetin were found at all stages in similarly lower concentrations. Concentrations of luteolin, quercetin and apigenin were 225, 410 and 690 % greater, respectively, in flowers than in leaves or stems; coumestrol concentration was similar between plant parts. In flowers and stems the predominant phytoestrogens were apigenin and quercetin, followed by coumestrol and luteolin. Similar concentrations (average 26 μg g^?1 DM) of each of the four phytoestrogens were found in leaves. Concentrations through the herbage canopy varied and were greatest at >60 cm from the soil surface for apigenin and coumestrol, but greatest at >60 and 0–20 cm for quercetin and at 0–20 cm for luteolin. The results suggest that if alfalfa is to be used as a source of phytoestrogens and is harvested for the production of herbal supplements or nutraceuticals, management will need to be adapted.     

辣椒夏季品比试验报告

为了考察我公司新推广品种及新配组合在高温伏旱状况下的适应性，我们于2003年5月7日在公司试验基地进行了品种对比试验。结果表明新推广品种中有九个品种或组合为极耐热或耐热，一个品种耐热性稍差。新配组合中部分表现优良，有推广前景。     

槟榔红色素的抗氧化活性

测定槟榔红色素(areca red pigment,AP)对 DPPH·和·OH的清除能力、对脂质体过氧化的抑制能力、对Fe2+的络合能力,并测定其还原能力.结果表明:槟榔色素对DPPH·、·OH的清除能力较强:对脂质体过氧化的抑制能力很强,抑制率高达79.84%;与VE、没食子酸(gallic acid,GA)、BHT相比,槟榔色素的还原力、对Fe2+的络合能力较弱.说明槟榔红色素是较好的抗氧化剂.     

山拖农装携手五征集团同心并力再奏市场强音

5月22日,备受行业内关注的山东五征集团山拖农机装备有限公司揭牌仪式在山东省兖州市举行.至此,中国两大农机巨头--五征集团和山拖农装真正实现了战略性联合.见证了建国初期农机艰苦创业和改革开放后市场经济激烈竞争的山拖农装,遍尝辉煌与低谷的洗礼,在历经半世纪的风雨后,凤凰涅檠,浴火重生.     

对书写工具的选择与预防近视的认识和建议

从字迹是视觉信号这个角度来说,中小学生写字时最好使用较软质的铅笔或用钢笔。如果说低头歪头写字达不到书写＂三个一＂的要求,是孩子们＂喜欢低头歪头＂,那么众多家庭从孩子小的时候就手把手地教孩子怎样握笔写字、上学后老师又是那么耐心地教学生如何正确执笔书写,反而还有如此多的学生还是很难达到书写＂三个一＂的要求呢？     

黄土低山丘陵及坨甸草原区风蚀规律的研究

本文通过对黄土低山丘陵及坨甸草原区248个风蚀图斑资料的分析,运用线性模型理论,对此地区的风蚀规律进行了研究。获得了下垫面各因素与风蚀量关系的数学模型。找出了影响风蚀的主导因素,并系统阐述了植被覆盖度和土地利用类型对风蚀量大小的影响,且与水蚀规律进行了比较分析。     

3种农药的微核效应研究

运用蚕豆根尖微核技术研究3种农药(甲胺磷、甲基硫菌灵和盐酸吗啉胍)在不同浓度下的诱变效应。结果表明,3种农药诱发蚕豆根尖细胞微核率与对照组相比均有不同程度的上升(P<0.05或P<0.01),农药浓度与微核率呈剂量-效应关系,盐酸吗啉胍的诱变效应大于其他2种农药。     

Landscape and habitat factors affecting cabot's tragopan Tragopan caboti occurrence in habitat fragments.

Cabot's tragopan Tragopan caboti is an endemic and endangered pheasant of the lower montane forests of southeastern China. The typical habitats of the tragopan have been seriously fragmented because of forest management for timber production and farmland reclamation in recent years. The effects of the fragment size and isolation on the distribution of the cabot's tragopan were studied in Wuyanling Natural Reserve. Thirty one habitat fragments (2.5-48.5 ha) surrounded by non-habitat sapling coniferous forests, in an intensively managed forested landscape, were surveyed over four seasons for the occurrence of cabot's tragopan. Five of the 31 fragments were occupied in all four seasons and nine were not occupied. Both landscape and habitat factors affected the occurrence of cabot's tragopan, with landscape factors having the greatest effect. Large and less isolated habitat fragments containing a larger amount of the tree Daphniphyllum macropodum were occupied significantly more often than small, isolated fragments. The appearance of cabot's tragopan in the habitat fragments was best explained by the size of the fragments, the distance to the nearest suitable habitat and the amount of macropdous daphniphyllum trees. Our results could be used to improve the management of the forests where Cabot's tragopan occurs in southeastern China.     

一种快速分析香蕉果实采后mRNA水平变化的新方法

采用SMARTPCRcDNA合成与计算机软件相结合的方法,快速分析香蕉果实采后mRNA水平的变化。在28℃条件下分别将处于同株同穗同梳相邻位置的香蕉果实放置24、48、72h。应用这种方法对其总mRNA水平进行了分析,结果表明,香蕉采后48h时,与采后0h相比,mRNA水平最高。该方法由于使用了计算机软件TheScionImage,可对cDNA电泳图进行数字化分析,具有用材少,敏感性高,快捷方便等特点,特别适用于对较多样品进行分析。