大白菜NPR家族基因鉴定及表达模式分析

为明确大白菜NPR转录辅助因子在大白菜基因组中的数量、结构和表达特点,揭示NPR在水杨酸信号途径调控大白菜生长发育过程中的作用。本研究利用拟南芥资源信息库TAIR10和芸薹属基因组网站Brassica Database鉴定大白菜基因组中的NPR家族成员,并对其蛋白和基因特性进行生物信息学分析。鉴定出大白菜基因组中包括11个NPR家族基因,分别定位在大白菜7条染色体上,具有1~5个外显子不等。BrNPR家族蛋白可聚类为6个分支,包含11个保守元件,并且所有BrNPR家族成员均含有DUF和Ank两个保守结构域,其中BrNPR1-4成员基因的编码区均含有核定位信号(NLS)。基因表达模式检测结果表明部分大白菜NPR基因在不同组织中具有组织特异的表达模式,BrNPR1B在根、茎和叶及BrNPR3B在花和种荚中表达显著,绝大部分BrNPR基因受盐胁迫诱导表达,部分BrNPR基因受根肿菌侵染诱导表达。本研究可为后续大白菜NPR基因的功能验证提供依据。     

Identification of N-linked Glycoproteins in Silkworm Serum Using Con A Lectin Affinity Chromatography and Mass Spectrometry

Glycosylation is one of the most common post-translational modifications to occur during protein biosynthesis, but remains poorly understood in insects. In this study, we collected serum proteins from two silkworm developmental stages, namely day 7 of the fifth instar larval stage and day 2 of the pupal stage. Results of SDS–PAGE and periodic acid-Schiff staining revealed that most serum proteins with high abundance were putative glycoproteins. LC-MS/MS identified 149 larval and 303 pupal serum proteins in the Con A lectin-enriched fractions. GO analysis revealed that many serum proteins were involved in the proteolysis and carbohydrate metabolic process. 82 N-linked glycoproteins with at least one glycosylation site were identified. N-Linked glycosylation occurred at the sequon, Asn-X-Ser/Thr, and the proportions of Ser and Thr glycosylation at the hydroxy position were found 39.6% and 60.3%, respectively. The N-glycan structures found in serum glycoproteins were mainly Man₂FucGlcNAc₂ (67.9%). Since storage protein 1 and transferrin had a relatively high abundance in the serum and could be significantly enriched by Con A lectin, their glycosylation was analyzed in detail. Glycoside hydrases, serine proteases and serpins were found to form three interacting glycoprotein networks using the website STRING. This study provides important clues for the understanding of the function of N-linked glycosylation in metabolism, immunity, and metamorphosis.     

蚕豆测土配方施肥指标体系初报

为探索、构建适宜生产实践的蚕豆测土配方施肥指标体系,为蚕豆生产提供科学施肥依据,以本地白花蚕豆、尿素、普钙、硫酸钾为试验材料,采用"3414"回归最优设计原理设置田间肥效试验,对蚕豆氮磷钾施肥效应进行了研究。结果表明:不同施肥处理对蚕豆产量、产值和蚕豆植株地上部分养分吸收量、100 kg蚕豆籽粒吸收养分量影响较大。处理7(N_2P_3K_2)较对照处理1(N0P0K0)增产量1600.50 kg/hm~2、增产值5601.75元/hm~2,为最高;其次分别是处理6(N_2P_2K_2)、处理9(N_2P_2K_1)、处理14(N_2P_1K_1),分别较对照增产量1450.5 kg/hm2、增产值5076.75元/hm~2;植株地上部分氮、磷、钾养分吸收量最高的是处理6,其次,是处理4(N_2P_2K_2)的磷、钾和处理8(N_2P_2K_0)的氮;100 kg籽粒吸收氮养分量处理2(N0P2K2)达6.26 kg为最高,其次是处理6达5.90 kg,最低的是处理8;100 kg籽粒吸收磷、钾养分量最高的分别是处理2和处理4,分别达0.76、3.07 kg;最低的是处理1和处理8。说明不同施肥处理是影响蚕豆植株吸收土壤养分、蚕豆产量的主要因素,合理施肥能有效提高蚕豆产量。氮肥增产率十分明显,1 kg纯氮增产蚕豆23.33 kg;磷、钾肥增产效果比较好,1 kg纯磷、1 kg纯钾分别增产蚕豆6.12、3.34 kg。说明蚕豆吸收养分的限制因子主要是施氮量,其次是施磷量。蚕豆土壤有效养分N、P_2O_5、K_2O校正系数分别为1.806711、0.80545、1.397613。     

云浮市城郊主要森林植被类型分布调查

对云浮市城郊主要森林植被类型进行调查，结果表明，在该地区有591种木本植物，构成9种植被类型；并分析了不同植被类型的结构特征，为地域性植被恢复提供参考。同时对如何加强原生植被的保护提出了意见。     

庆元杉木种子园投资结构及经济效益研究

用动态经济评价方法对浙江省南部的庆元杉木种子园的投资结构及经济效益进行了评价。结果表明：（１）该园在２０ａ生产周期内生产性投资６１．６５万元，占种子园总投资的６６．３１％，基建、设备投资占３３．６９％。其中１５ａ产种期投资为５２．０２万元，占良种生产区总投资５７．６６万元的９０．２２％；（２）该园在２０ａ生产周期内销售种子收入达６４．４１万元，就此一项就回收了６１．６５万元的生产性投资。净现值为２９．６６万元，内部收益率达８．０％，成本效益率为１．４２元，投资回收期为１９．２５ａ；（３）该园良种子代材积的遗传增益为１５．３９％，因此宏观静态评价为，每投资种子园１元钱成本，可使良种使用者在１个轮伐期后获得１８０．７０元的额外收益，该种子园经济效益和社会效益均十分显著     

沙菜胶的初步研究

本文报道了对沙茶胶在提取方法、性质和应用等方面的初步研究结果。其提取方法是先将沙茶置于室温条件下用波美20°的Na0H处理2天,水洗后加入15-20倍0.04%的硫酸,100℃煮胶1-1.5小时,加入KCl至0.2—0.3%过滤(或过滤后加KCl),滤波放置凝固、冷冻、解冻,最后干燥后得产品。电解质对沙菜的凝固强度影响很大,对提高凝胶强度,阳离子的顺序是:Ca~( )>K~ ,Cs~ ,Rb~ >Na~ ,Li~ ,NH_4~ ;阴离子的顺序是:Cl~->NO_3~-,SO_4~->I~->CO_3~-。沙菜胶的凝固温度与加入的KCl浓度有关,KCl浓度大,凝固温度也随之升高。试验了四种细菌在沙菜胶培养基上的生长情况,结果表明细菌在沙菜胶培养基上生长的活菌计数甚至比在琼脂培养基上的还多。     

三门湾潜堤网栏养殖对虾的初步试验

塔堤网栏养殖方式,曾由中国水产科学研究院渔业工程研究所利用国产设备,1986年在黄渤海区粉砂底质较为开阔的海区试验成功。本试验选在台风较为频繁的东海区浙江三门湾进行。三门湾由许多小港湾组成,沿岸滩涂广阔,近岸水域饵料生物相当丰富,水质清。     

云岭山羊皮肤组织结构的观察

 本文报道了分布于云南省曲靖地区的云岭山羊皮肤组织结构的特点,其结果是:皮肤总厚度1655μm,真皮毛囊层厚435.5μm,网状层厚1160μm。次级毛囊短,一般只达初级毛囊皮脂腺水平处。1mm²皮肤面积初级毛囊达2.97个;次级毛囊与初级毛囊数比为6.6。同时,对该品种羊的皮用质量问题进行了讨论。     

0.2%苦皮藤素乳油防治茶尺蠖等害虫药效试验研究

【研究目的】研究植物源类杀虫剂苦皮藤素乳油对茶尺蠖等害虫防治效果。【方法】应用0.2%苦皮藤素乳油,500倍、1000倍和1500倍3个浓度,分别处理茶鲜叶放入果酱瓶中,接上茶尺蠖二龄幼虫,进行室内防效评价实验;应用1500倍进行大田防效试验;应用500倍、1000倍和1500倍3个浓度进行防治茶小绿叶蝉的田间防效小区对比试验。【结果】应用500倍、1000倍和1500倍3个浓度的0.2%苦皮藤素其结果表明;室内:0.2%苦皮藤素500倍,药后3天死亡率98.67%,1000倍的死亡率85.33%,1500倍的死亡率73.33%;大田防效:0.2%苦皮藤素1500倍药后10天防治茶尺蠖,防效为88.13%～90.69%;0.2%苦皮藤素500倍药后8天防治小绿叶蝉,防效为63.66%～80.11%。【结论】试验显示了该药剂对茶尺蠖有较好的防效和持效期,可作为植物源类杀虫剂在无公害茶叶生产基地上推广应用。     

Stored light in an optical fiber via stimulated Brillouin scattering

We describe a method for storing sequences of optical data pulses by converting them into long-lived acoustic excitations in an optical fiber through the process of stimulated Brillouin scattering. These stored pulses can be retrieved later, after a time interval limited by the lifetime of the acoustic excitation. In the experiment reported here, smooth 2-nanosecond-long pulses are stored for up to 12 nanoseconds with good readout efficiency: 29% at 4-nanosecond storage time and 2% at 12 nanoseconds. This method thus can potentially store data packets that are many bits long. It can be implemented at any wavelength where the fiber is transparent and can be incorporated into existing telecommunication networks because it operates using only commercially available components at room temperature.