p38MAPK信号通路选择性调节FSH对甾体生成 的诱导作用研究

利用乙烯雌酚（DES）处理23日龄SD大鼠，分离卵巢颗粒细胞(GC)进行无血清培养。结果表明，FSH (50ng/m1) 处理GC，可迅速激活有丝分裂原蛋白激酶（p38 MAPK），FSH处理5min便可观察到磷酸化的p38 MAPK；FSH处理30min，磷酸化的p38 MAPK水平达到最高;向培养液中加入H89(蛋白激酶A抑制剂，10μM)，则显著抑制了FSH对p38 MAPK的激活作用，提示这种激活作用依赖于蛋白激酶A(PKA)。用 SB203580(p38MAPK抑制剂，20μM)抑制p38 MAPK激活，则进一步提高了FSH对孕酮和甾体生成快速调节蛋白（StAR）的诱导作用，同时降低了FSH对雌激素生成的促进作用(p<0.01)。RT-PCR结果显示：抑制p38 MAPK活性后，FSH对StAR mRNA刺激作用明显增强，但对细胞色素P450芳香化酶（P450arom）mRNA的诱导作用却减弱了(p<0.05)。激光共聚焦和蛋白印迹结果显示：在GC中，StAR蛋白主要分布在线粒体中；与对照组相比，FSH显著提高了StAR的荧光强度和蛋白水平；抑制p38MAPK活性则增强了FSH对StAR蛋白表达的诱导作用。     

ANALYSES FOR DEFECTS OF 18G_2ANb STEEL SPHERICAL TANKS OF NATURAL GAS

Calculations and analyses are made with the CVDA, IIW, JWES standards for the strength and welding defects of two spherical tanks of natural gas in CHONG QING. The results of the analyses indecate that though there are some problems with the welding quality in these tanks, they may still be used with the desingned presure. Now the formal working of these two spherical thanks has verified of the validity the calculations and analyses.     

A Globally Convergent Algorithm for Nonlinear Programming

A new globally convergent algorithm was presented for a continuous differen-tiable nonlinear programming by defining a measure function deviating from the Kuhn-Tucker point. With this algorithm which can be used to get the optimum solution of the problem,the optimal lagrangian multiplier corresponding the optimal solution of the problem was also obtained. A new iterative algorithm for quadratic programming is obtained when applying the general algorithm to quadratic programming. Finally,a numerical example was given.     

Influence of the Flood-Control-Projects on the Hydrodynamic State of the Rivers Confluence in Chongqing

Though the Flood-Control-Projects along Yantze River and Jialing River in Chongqing may well improve the urban traffic and construction,it may change the river hydrodynamic characters.There may be two consequences,one is to reduce the size of cross section and the other is to change the river shape,such as the curvature radius.The phenomena of the local backwater and velocity variety are obvious,which result in some problems for the navigation: sediment,transport and so on.In this paper, the study object is the Flood-Control-Projects and Yangtse River and Jialing River,and the aim is to study the influence of the Flood-Control-Projects on the hydrodynamic state of the two rivers.Using the numerical technique,with the two rivers hydrodynamic model the flow field's change of river after completion of Flood-Control-Projects is simulated and the reasons caused by the change are analyzed.     

粉花石斛组织培养快速繁殖

采用种子培养(胚培养)和茎节培养直接诱导成苗的方式进行了粉花石斛组织培养快速繁育试验.试验结果表明,无论是以种子还是以茎节作为外植体,均能诱导成苗.种子培养的最适培养基为N6 6-BA 5 NAA 0.4;茎节培养的最适培养基为I/2MS 6-BA 0.9 NAA 0.2;适宜的生根培养基分别为:N6 10%香蕉汁、1/2MS.移栽最适基质配方为腐叶土:珍珠岩为1:1.     

湿式多盘制动器的研究现状及展望

分别对国内外在湿式多盘制动器摩擦副间对流换热机理、摩擦衬片压力分布规律、摩擦偶件温度场与应力场的分析、摩擦衬盘与对偶钢盘的摩擦机理、制动噪声、制动过程的影响因素、整体散热特性及其试验研究等方面的研究现状进行了综述和评价，并对湿式多盘制动器的某些研究内容提出了新的见解，最后展望了湿式多盘制动器的研究方向及其发展动态。     

创新教学方法培养创新人才

培养具有创新能力的优秀人才是对高等教育的本质要求：学生创新能力的培养具有多种方式，其中，创新教学方法是培养创新人才的一种有效途径。我们在农业工程类专业课程教学中，积极进行教学方法的创新，鼓励学生进行自主性教学活动，提高了学生的创新能力，收到了很好效果。     

标准型(三行星轮)复合齿轮转子泵的机理研究

概述了普通齿轮转子泵的结构及工作原理．介绍了标准型(三行星轮)复合齿轮转子泵的工作原理，分析了该泵的力学特性，推出了平均流量和瞬时流量公式，并对该泵的脉动率进行了分析，得出当工作轮的齿数为非3的倍数时，该泵的流量脉动将显著降低的结论。当泵的工作轮及腔体用特殊材料(如不锈钢或陶瓷)制作或处理时，该泵完全可以用在纯水液压系统。     

棱锥型孔排种部件的研究

根据地膜覆盖精量播种玉米的要求，研制了棱锥型孔排种部件，介绍了它的工作原理及基本参数，经台架试验得出了该排种器结构参数的较优设计方案。在较优设计参数下，棱锥型孔排种圆盘的线速度ｖ＝０．８０７ｍ／ｓ时，单粒率为９３．６７％，空穴率为１．６７％，其指标达到了机械式膜上玉米精量播种的先进水平，为地膜覆盖播种玉米提供了一种机械式精量排种器。     

工程车辆自动变速控制系统仿真与试验

对液力机械传动工程车辆遇到高强度载荷效率下降的问题进行了研究，推导了新的换挡规律。按照预先设定的经济型换挡规律，利用推导出的不同挡位下换挡点的通用公式，可将变矩器的效率限定在某一理想范围内。获取对换挡离合器执行机构的控制来达到对离合器油压的控制目的，仿真结果表明其跟踪效果良好。通过台架试验，取得了满意的效果。