尽快建立森林生态效益补偿制度——对福建江西两省相关问题的调查

分别调查了福建、江西省实施林业分类经营改革试点和生态公益林区划、界定和管护的主要作法 ;指出了在目前国家没有建立森林生态效益补偿机制的情况下 ,实施分类经营和加强生态公益林保护面临的困难和问题 ;对森林生态效益补助资金的补偿对象、范围、标准 ,补助资金的管理形式 ,补助资金与现有生态公益林管护资金的衔接等问题进行了研究。通过调研提出统一认识 ,尽快建立森林生态效益补偿制度等 5点政策建议。     

花粉中酶的研究

花粉中酶的研究王开发张玉兰张盛隆（同济大学花粉应用研究中心）酶是影响细胞代谢的重要物质，是摄入生物体内的营养成分进行分解、合成时起催化作用，而称为生物催化剂。但酶与其它催化剂不同，能在机体十分温和的条件下高效率地起催化作用，使生物体内的各种物质处于不...     

液相色谱/酶标免疫分析法测定动物组织中已烯雌酚

青岛黄海水产研究所２６６０００ 欧盟及国际社会对在动物性食品严禁使用１，２一二苯乙烯类药物。我国也相应制定了严禁在肉食动物中使用己烯雌酚（ＤＥＳ）等激素的法规。继而我国于 １９９９年制定了《中华人民共和国动物及动物源食品残留监控计划》，加强了对违禁药物的监控力度，规定动物产品中１，２－二苯乙烯类药物的最大残留限量（ＭＲＬ）为不得检出。目前，检测动物产品中ＤＥＳ残留量的方法较多，有紫外分光光度法、液相色谱法、放射免疫法、ＧＣ／ＭＳ法等。各方法各有优缺点。本文根据残留监控过程中既要大批量快速测定，又…     

两种一年生植物在替代试验中的相互竞争关系

（1）用替代试验研究了燕麦（Aneva sativa）和箭舌豌豆（Vicia sativa）在混播中的竞争相互作用。为了攻混播比例对两种植物在混播条件下的行为影响，田间试验设为5个混播总密度和5种混播比例，共了5次。（2）方差分析表明，相对总生物量（RYT）和竞争平衡指数（CBI）对总密度，混播比例和生长时间的反应敏感。总密度对RYT和CBI的影响高度显著，在大多数情况下，混播比例对RYT和CBI的影响不显著。（3）本文研究的结果表明，持续时间对RYT和CBI很强的影响，在第1次测定时，RYT值接近1而BI值接近0，这一事实表明，种间无竞争或互惠作用。而在生长旺盛期，种间竞争较为剧烈。（4）本研究结果表明，箭舌豌豆的生长严惩受到燕麦的抑制作用，这说明箭舌豌豆在与燕麦混播时表现为竞争上的弱者。     

The effect of exploratory laparotomy on the serum and peritoneal haptoglobin concentrations of the pony.

Serum haptoglobin concentration was used as an indicator of the acute phase response in ponies undergoing exploratory laparotomy. Preoperative, 1 h intraoperative, 3 h, 6 h, 12 h and 24 h postoperative blood samples and 48 h postoperative peritoneal fluid samples were obtained for haptoglobin analysis. A spectrophotometric assay based on cyanmethemoglobin binding capacity (CyanBC) was used to determine haptoglobin concentrations. The preoperative reference range for serum haptoglobin concentrations in these ponies was 25-60 mg CyanBC/dL. Intraoperative and 3 h postoperative blood samples had decreased haptoglobin concentrations when compared to preoperative values. Serum haptoglobin concentrations began to rise by the 6 h postoperative sample and were generally elevated above preoperative values by the 24 h postoperative sample. Two of the ten ponies had mild signs of postoperative colic which were associated with twofold elevations in serum haptoglobin concentrations and fivefold elevations in peritoneal fluid haptoglobin concentrations.     

桑悬浮细胞原生质体培养的研究

采用继代培养三个月后的桑子叶悬浮细胞为材料,进行了原生质体的分离和培养。桑悬浮细胞在纤维素酶、果胶酶、半纤维素酶的混合溶液中,酶解获得产量高、活力强的原生质体。原生质体在K8p(附加6—BA、NAA、2,4—D、LH)液体培养基中,再生细胞经多次分裂,得到肉眼可见的小愈伤组织。再通过增殖继代培养,获得浅黄色、具有明显颗粒结构的愈伤组织,转至各种激素含量的MSB固体培养基中,尚未获得绿苗分化。     

Feline leukemia virus detection by immunohistochemistry and polymerase chain reaction in formalin-fixed, paraffin-embedded tumor tissue from cats with lymphosarcoma.

The prevalence of feline leukemia virus (FeLV) antigen and DNA was assessed in formalin-fixed, paraffin-embedded tumor tissues from 70 cats with lymphosarcoma (LSA). Tissue sections were tested for FeLV gp70 antigen using avidinbiotin complex (ABC) immunohistochemistry (IHC); DNA was extracted and purified from the same tissue blocks for polymerase chain reaction (PCR) amplification of a 166 base pair region of the FeLV long terminal repeat (LTR). Results were related to antemortem FeLV enzyme-linked immunosorbent assay (ELISA) for serum p27 antigen, anatomic site of LSA, and patient age. Viral DNA was detected by PCR in 80% of cases and viral antigen by IHC in 57% of cases. Seventeen cases were PCR-positive and IHC-negative; one case was PCR-negative and IHC-positive. Clinical records included FeLV ELISA results for 30 of 70 cats. All 19 ELISA-positive cats were positive by PCR and IHC; of the 11 ELISA-negative cats that were negative by IHC, seven were positive by PCR. When evaluated according to anatomic site, FeLV DNA and antigen were detected less frequently in intestinal LSAs than in multicentric and mediastinal tumors. Lymphosarcoma tissues from cats < 7 yr were several fold more likely to be positive for FeLV antigen by IHC than were tumors from cats > or = 7 yr. However, there was no significant difference in PCR detection of FeLV provirus between LSAs from cats < 7 yr and those > or = 7 yr.(ABSTRACT TRUNCATED AT 250 WORDS)     

饲粮不同蛋白质、能量水平对大白鼠生长发育及体成分的影响

本试验所用动物为53—57日龄雄性大白鼠(sprague——Dawley品系),初始体重为121.73±3.95g,试验期为大白鼠生长发育旺期的21天。试验拟研究不同粗蛋白水平(10％、15％、20％、25％和30％)及两个能量水平(消化能:3.6和3.9kcal/g)的饲粮对大白鼠生长发育及各种体成分的影响。结果表明,当饲粮蛋白质供应充分时,大白鼠生长随饲粮能量浓度的提高而改善;而当饲粮蛋白质供应感觉不足时,提高能量浓度对生长无益。机体各项成分指杯中,以蛋白质、脂肪、水分沉积受饲粮蛋白质,能量浓度影响显著;而蛋白质、脂肪沉积在很大程度上受饲粮能朊比的影响,饲粮中添加脂肪可使大白鼠机体肥胖。