全文获取类型
收费全文 | 137816篇 |
免费 | 20751篇 |
国内免费 | 31272篇 |
专业分类
林业 | 16349篇 |
农学 | 19307篇 |
基础科学 | 13960篇 |
40595篇 | |
综合类 | 50467篇 |
农作物 | 9591篇 |
水产渔业 | 7996篇 |
畜牧兽医 | 17047篇 |
园艺 | 4343篇 |
植物保护 | 10184篇 |
出版年
2024年 | 987篇 |
2023年 | 1674篇 |
2022年 | 3220篇 |
2021年 | 3974篇 |
2020年 | 5178篇 |
2019年 | 8247篇 |
2018年 | 7270篇 |
2017年 | 8731篇 |
2016年 | 9069篇 |
2015年 | 9769篇 |
2014年 | 9004篇 |
2013年 | 10224篇 |
2012年 | 10688篇 |
2011年 | 10197篇 |
2010年 | 9132篇 |
2009年 | 8196篇 |
2008年 | 7048篇 |
2007年 | 7592篇 |
2006年 | 6993篇 |
2005年 | 6371篇 |
2004年 | 4118篇 |
2003年 | 3564篇 |
2002年 | 3013篇 |
2001年 | 2888篇 |
2000年 | 3010篇 |
1999年 | 3286篇 |
1998年 | 3100篇 |
1997年 | 2726篇 |
1996年 | 2515篇 |
1995年 | 2500篇 |
1994年 | 2314篇 |
1993年 | 1985篇 |
1992年 | 1820篇 |
1991年 | 1531篇 |
1990年 | 1239篇 |
1989年 | 1051篇 |
1988年 | 893篇 |
1987年 | 650篇 |
1986年 | 500篇 |
1985年 | 259篇 |
1984年 | 231篇 |
1983年 | 226篇 |
1982年 | 212篇 |
1981年 | 265篇 |
1980年 | 203篇 |
1979年 | 155篇 |
1978年 | 130篇 |
1975年 | 109篇 |
1965年 | 139篇 |
1964年 | 141篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
161.
162.
163.
E M Aburto A Cribb I C Fuentealba B O Ikede F S Kibenge F Markham 《Canadian journal of veterinary research》2001,65(2):104-110
This study evaluates the ability of selenium (Se) supplementation to prevent experimental copper (Cu)-induced hepatocellular damage. Weanling male Fischer 344 rats were randomly assigned to groups of 15, 3 groups (A,B,C) were fed Cu-loaded diets (containing 2000 microg/g copper, added as CuSO4) and different levels of Se (added as Na2SeO3 x 5H2O) as follows: A) Cu-loaded/Se adequate diet (0.4 microg/g Se, fed basis); B) Cu-loaded/Se-supplemented diet (2 microg/g Se); and C) Cu-loaded/Se-deficient diet (< 0.2 microg/g). Three additional groups (D,E,F) were fed diets containing adequate levels of Cu (14 microg/g Cu, fed basis) and different levels of Se as follows: D) Cu-adequate/Se-adequate diet; E) Cu-adequate/Se-supplemented diet (2 microg/g Se); and F) Cu-adequate/Se-deficient (< 0.2 microg/g) diet. After 4, 8, and 12 weeks on the experimental diets, liver samples were processed for histology, histochemistry, metal analysis, glutathione peroxidase (GSH-Px) measurement, and quantification of malondialdehyde (MDA). Morphologic changes characteristic of Cu-associated hepatitis, without an increase in hepatic MDA levels, were seen in all Cu-loaded rats in each sampling. Similar changes occurred in rats fed Se-adequate, Se-supplemented and Se-deficient diets. This study demonstrates that Fischer 344 rats fed 2000 microg/g Cu develop morphologic changes due to Cu toxicity without evidence of lipid peroxidation. Furthermore, Se supplementation does not result in protection against Cu-induced liver injury. 相似文献
164.
结核病与布鲁氏杆菌病都是对人体健康有严重危害的人畜共患传染病。据卫生部门统计,我国现有结核病病人近600万人,每年因结核病死亡的约25万人,是单一病菌引起死亡最多的传染病。仅1998年第三季度新疆结核病发现人数为 4 794人,由此可见,此病对人类健康有较大的威胁。而布鲁氏杆菌病同样是人畜共患的全身性传染病,且结核病和布鲁氏杆菌病的传播途径相同,由于人类的结核和畜禽结核有较强的相互交叉感染性,结合我地区实施“放心肉、放心奶”跨世纪“牧业工程”,我们于2000年春、秋两季对哈密市郊区的500余头奶牛… 相似文献
165.
用铬变素2R法对家蚕微孢子虫孢子染色的研究 总被引:4,自引:1,他引:3
采用铬变素 2R法 (Chromotrope 2R)染色家蚕微孢子虫 (Nosemabombycis,N .b)孢子和绿僵 (Nomuraearile yi)孢子、曲霉 (Aspergillusflavus)孢子、花粉粒 (Pollengranule)等与N .b孢子形状相似物 ,结果表明 :Chromotrope 2R法特异性地将N .b孢子染成粉红色 ,绿僵孢子、曲霉孢子、花粉粒未被染成红色 ,初步认为Chromotrope 2R法可应用于母蛾镜检的N .b鉴定 ,能明显提高N .b的检出率与准确性 ;染色N .b孢子的最佳时间与温度为 4 0min ,30℃。鉴于本染色法的条件、技术操作简便 ,染色特异性强 ,因而具有良好的应用前景 相似文献
166.
167.
乡镇企业如何走出目前的经营困境,如何确定自己的战略选择,不同的人会有不同的认识。笔者试图从经营理念和管理者收购这一“软”一“硬”两方面做一些探讨。 相似文献
168.
Differences in Mycoplasma hyopneumoniae colonization were evaluated in experimentally inoculated pigs sired by 3 different boars of the same genetic line. Forty-six pigs were used, including a treatment group and positive and negative control groups. The pigs were intratracheally inoculated with an M. hyopneumoniae suspension or with Friis media as a placebo. To evaluate differences in the magnitude of colonization during a 35-day period, nasal and tracheal swabs were collected weekly and tested by nested polymerase chain reaction (N-PCR). Temperature, weight and circulating antibodies were measured for 35 days. At 11 and 35 d postinoculation the pigs were necropsied and macroscopic and microscopic lesions were determined. A section of bronchus was tested by the indirect immunofluorescence test (IFAT), scanning electron microscopy (SEM) and N-PCR. The N-PCR results from the nasal and tracheal swabs showed that the pigs sired by one boar (B3) had a distinctive colonization pattern, different from that of the pigs from the other 2 boars and from the positive controls. SEM studies demonstrated that at 35 d postinoculation a higher proportion of B3 pigs had lower numbers of mycoplasmas attached to the cilia compared with B1 and B2 offspring. No significant differences were observed in temperature and weight gain among groups by ANOVA; however, with use of a 2 × 2 table, temperature differences were observed between pigs sired by boars B1 and B2 at 4 d postinoculation. No pigs seroconverted, showed gross or microscopic lesions, or had positive IFAT results. These results provide evidence of differences in patterns of colonization between pigs sired by different boars, suggesting a possible genetic effect. 相似文献
169.
为适应按库配水,按方收费的需要,达到节约用水的目的,研制了自锁封闭式启闭机闸门。该装置将闸门和启闭机融为一体,封闭于地下,易操作,节水。介绍了其工作原理、工程结构和施工方法。 相似文献
170.
Capnographic documentation of nasoesophageal and nasogastric feeding tube placement in dogs 总被引:1,自引:0,他引:1
Paula A. Johnson DVM F. A. Mann DVM MS DACVS DACVECC John Dodam DVM MS PhD DACVA Keith Branson DVM MS DACVA Colette Wagner-Mann DVM PhD Mark A. Brady DVM Elizabeth Dunphy DVM 《Journal of Veterinary Emergency and Critical Care》2002,12(4):227-233
Objective: To evaluate the ability of capnography to document proper placement of nasoesophageal (NE) and nasogastric (NG) feeding tubes. This study was conducted in 3 phases. Phase I of this study was designed in order to test the efficacy of capnography to distinguish placement of a feeding tube in the alimentary tract versus the respiratory tract. Phase II was designed in order to document that carbon dioxide (CO2) could be measured through a polyvinyl chloride (PVC) feeding tube. Phase III was performed in order to evaluate the technique of continuous monitoring during insertion of the feeding tube into the esophagus and stomach as would be performed during a clinical‐tube placement. Design: Prospective study. Setting: Research laboratory. Animals: 24 adult dogs. Interventions: In Phase I, sedated dogs were instrumented with an intratracheal catheter and an 8 French feeding tube placed nasally into the distal esophagus and later advanced into the stomach. In Phase II, dogs were anesthetized and an 8 French feeding tube was placed down the endotracheal tube, then into the esophagus and later advanced into the stomach. In Phase III, sedated dogs were instrumented with an 8 French feeding tube inserted intranasally and then advanced to the level of the nasopharynx, distal esophagus and, lastly, the stomach. Fluoroscopy was used in order to determine location of the feeding tube. Measurements and main results: Phase I measurements included respiratory rate and CO2 from the trachea, esophagus, and stomach and pH of gastric fluid sample. Phase II measurements included respiratory rate and CO2 from the endotracheal tube, feeding tube in the endotracheal tube, feeding tube in the distal esophagus, and feeding tube in the stomach. Phase III data collection included respiratory rate and CO2 as the tube was passed through the nasal cavity, nasopharynx, esophagus and stomach. Phase I fluid samples were collected from 5 of the 9 dogs and had pH values from 1.68 to 4.20. In both phases, values for the respiratory rate and CO2 from the esophagus and stomach were 0 ± 0, significantly lower (P < 0.001) than the values from the trachea. In Phase II, there was no significant difference between the respiratory rates (P = 0.886) and CO2 (P = 0.705) readings obtained from the endotracheal tube compared to readings from the feeding tube in the endotracheal tube. In Phase III, there was a significant difference (P < 0.001) between the respiratory rates and CO2 readings obtained from the nasal cavity and the nasopharynx when compared to those readings obtained from the esophagus and stomach. Measurement of CO2 and respiratory rate resulted in a reading of 0 every time the feeding tube was in the esophagus or stomach. Conclusions: Capnography may be used in order to detect airway placement of NE and NG tubes. 相似文献