Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.

Previous studies showed that the yellow seed color gene of a yellow mustard was located on the A09 chromosome. In this study, the sequences of the molecular markers linked to the yellow seed color gene were analyzed, the gene was primarily mapped to an interval of 23.304 to 29.402M. Twenty genes and eight markers’ sequences in this region were selected to design the IP and SCAR primers. These primers were used to screen a BC₈S₁ population consisting of 1256 individuals. As a result, five IP and five SCAR markers were successfully developed. IP4 and Y1 were located on either side of the yellow seed color gene at a distance of 0.1 and 0.3 cM, respectively. IP1, IP2 and IP3 derived from Bra036827, Bra036828, Bra036829 separately, co-segregated with the target gene. BLAST analysis indicated that the sequences of newly developed markers showed good collinearity with those of the A09 chromosome, and that the target gene might exist between 27.079 and 27.616M. In light of annotations of the genes in this region, only Bra036828 is associated with flavonoid biosynthesis. This gene has high similarity with the TRANSPARENT TESTA6 gene, Bra036828 was hence identified as being the gene possibly responsible for yellow seed color, in our research.     

浅议农业科研单位固定资产如何计提折旧

文章针对农业科研单位固定资产折旧作了初步分析,从固定资产计提折旧的重要性、计提方法、会计核算及存在的问题等方面进行了一些探讨。     

中国煤炭生产甲烷排放现状及对策研究

指出了煤炭行业的甲烷排放也是造成温室效应的重要因素之一,气候的变化直接影响到人类社会的可持续发展,我国有着丰富的煤层气储藏量,为发展新清洁能源奠定了坚实的基础,但是由于政策和技术限制,我国煤炭行业每年向大气中排放了大量的煤炭甲烷气体,尤其是低浓度煤炭甲烷,造成了资源浪费的同时也是煤炭生产安全的隐患.结合我国煤炭甲烷排放特征,分析了我国煤炭甲烷排放现状和主要的利用技术,并提出了中国煤炭甲烷排控的对策.     

青葙高效再生体系的建立

以青葙茎段为外植体建立高频再生体系,探讨不同激素配比对青葙不定芽诱导、继代增殖和不定根诱导的影响。结果表明:青葙茎段外植体诱导培养基为MS+6-BA(2mg/L)+IBA(0.5mg/L),诱导率为100%;继代增殖培养基为MS+6-BA(2mg/L)+IBA(0.01~0.02mg/L),不定芽增加3~5个;不定根诱导培养基为1/2MS+NAA(0.5mg/L)+IBA(0.5~1.0mg/L),诱导率高达100%;组培苗移植到盛有细沙的花盆中,成活率为100%。     

滨城区中水回用可行性分析

指出了滨州市滨城区水资源短缺严重制约着城市的快速发展,为了解决水资源的供需矛盾,开辟新水源势在必行.通过滨城区水资源现状、解决途径、中水回用的潜力及经济规模预测,阐述了滨城区中水回用的可行性.结果表明:采用中水回用措施每年可提供4.7亿m3杂用水,能够解决当前滨城区水资源供需矛盾;中水回用运行成本约在1.57元/m3,价格较为低廉,经济效益明显.可见中水回用不仅节约水资源也产生明显的经济效益,是城市发展的趋势所在.     

Regulatory effect of salicylic acid and methyl jasmonate supplementation on ergosterol production in Hericium erinaceus mycelia

We evaluated the biomass and ergosterol content of Hericium erinaceus mycelium, and extracellular enzyme activities in H. erinaceus liquid culture following salicylic acid(SA) and methyl jasmonic acid(Me JA)supplementation. The optimal SA concentration was100 lmoláL-1, where the highest ergosterol content of 2.33 mgág-1was obtained following 6-day cultivation with100 lmoláL-1SA supplementation, and which was significantly higher than the unsupplemented control(p \ 0.01). Following 4-day supplementation with50 lmoláL-1Me JA, the highest ergosterol content obtained was 1.988 mgág-1, which was 25.8 % higher than the unsupplemented control. Our data indicate that SA and Me JA supplementation improves ergosterol content in H.erinaceus mycelium.     

凤凰单丛茶树资源遗传多样性的ISSR分析

采用ISSR分子标记技术对48份凤凰单丛茶品种(系)进行了遗传多样性分析。选用10个多态性高、分辨力强的ISSR引物分别对供试材料基因组DNA进行扩增,共获得121个位点,其中多态位点带98个,多态位点比率(PPB)为81.0%。品种(系)之间的遗传相似系数变化范围在0.590 9~0.967 4之间。UPGMA聚类分析结果表明,在GS值为0.792的水平上供试材料可划分为7大类,其亲缘关系远近与地理来源关系不大且与香味类型没有必然的联系。     

粮食烘干塔换热器智能折边设备研发

结合螺旋板式换热器的应用领域及其板材折边生产现状,分析了其生产弊端。针对板材折边工艺要求,研发了粮食烘干塔换热器智能折边设备,并剖析其工作机理。根据典型产品结构参数,设计智能折边装备总体装配图并计算主要零件参数,为智能折边装备制作提供理论依据,从而实现换热器板材折边智能加工,提高换热器卷板折边质量及精度。     

钙果物理参数和生物力学特性试验

为减少钙果在收获中的机械损伤，对品系为农大5号的钙果果实的3轴尺寸、密度、结实度以及采摘力进行了分析。结果表明:农大5号在采收时期的平均粒径大小呈正态分布；随着果实成熟度的增大，果实的结实度会降低；果实的抗挤压能力具有各向异性，顶部受到的抗压力最大，纵向次之，横向最小；果实成熟前期在受到横向、纵向及顶部压力时，其变异系数分别为27.0%、27.5%和23.9%；随着加载速率的提高，摘果的平均力呈下降趋势，表现为拉力在5 mmmin时最大，15 mmmin时次之，45 mmmin时最小；对农大5号钙果果实的采摘拉力进行回归模型分析，其决定系数为0.995 9。试验结果可为钙果采摘装置的结构参数和工作参数提供试验基础依据。