Identification of qRL7, a major quantitative trait locus associated with rice root length in hydroponic conditions

Root system development is an important target for improving yield in rice. Active roots that can take up nutrients more efficiently are essential for improving grain yield. In this study, we performed quantitative trait locus (QTL) analyses using 215 recombinant inbred lines derived from a cross between Xieqingzao B (XB), a maintainer line with short roots and R9308, a restorer line with long roots. Only a QTLs associated with root length were mapped on chromosomes 7. The QTL, named qRL7, was located between markers RM3859 and RM214 on chromosome 7 and explained 18.14–18.36% of the total phenotypic variance evaluated across two years. Fine mapping of qRL7 using eight BC₃F₃ recombinant lines mapped the QTL to between markers InDel11 and InDel17, which delimit a 657.35 kb interval in the reference cultivar Nipponbare. To determine the genotype classes for the target QTL in these BC₃F₃ recombinants, the root lengths of their BC₃F₄ progeny were investigated, and the result showed that qRL7 plays a crucial role in root length. The results of this study will increase our understanding of the genetic factors controlling root architecture, which will help rice breeders to breed varieties with deep, strong and vigorous root systems.     

棉花油分合成相关基因的SSCP标记开发

本研究根据棉花油份合成相关基因(lysophosphatidic acid acyltransferase,LPAAT; diacylglycerol acyltransferase,DGAT; phosphoenolpyruvate carboxylase,PEPC)设计了75对引物,在8个棉花品种中运用SSCP技术...     

Potential use of a transposon Tn916-generated mutant of Aeromonas hydrophila J-1 defective in some exoproducts as a live attenuated vaccine

Aeromonas hydrophila is a Gram-negative opportunistic pathogen that causes disease in a wide range of hosts due to its multifactorial virulence. Here we describe the application of transposon insertion mutagenesis approach to obtain an exoenzyme mutant of A. hydrophila strain J-1. Immunization of swordtail fish (Xiphophorus helleri Heckel) with the highly attenuated mutant provided protection (survival of 27 out of 35 fish, compared to survival of only 13 out of 35 control fish) in the fish given the highest immunization dose (10(7) CFU) against intraperitoneal challenge with the wild J-1 strain. Immunization with doses of 10(5) or 10(3) did not provide significant protection.     

777超声辅助提取燕麦麸皮中β-葡聚糖的工艺研究

以燕麦麸皮为原料,采用超声波法提取分离β-葡聚糖,采用刚果红分光光度比色法测定及计算β-葡聚糖的含量。在考察提取时间、料水比、温度和提取次数等单因素的基础上,进一步进行多因子正交试验,优化提取工艺。确定最佳工艺条件为料水比为1∶15,提取时间为15min,提取次数为3次,提取温度为70℃,β-葡聚糖的提取率可达80.23%,得率可达7.32%。     

吉林省日光温室钢骨架的受力性能研究

针对目前吉林省内稍大跨度的日光温室产品抗风雪能力弱的现状,阐述了7.5~9m跨度钢骨架的几何设计参数、形状参数及选材方法。通过对比分析管材和钢筋的耗钢量、抗弯刚度及抗腐蚀性等特征,确定管材替代钢筋投入日光温室使用能有效提高其经济效益。同时,利用同济大学3D 3 S软件,以8m跨度钢骨架为例,分析了给定结构形式的受力性能及选材截面尺寸,为新建者提供参考。     

‘豫杂一号’泡桐叶绿体基因组序列及特征分析

泡桐是中国重要的速生树种,对我国经济发展有着重要作用。以‘豫杂一号’泡桐为材料,IlluminaHiseq2000测序,对其全基因组序列基本结构特征、密码子偏好性和重复序列进行统计分析。结果表明,‘豫杂一号’泡桐为典型的四分体结构,全长序列为154615bp,编码114个基因,含4个rRNA,30个tRNA和80个蛋白质编码基因。该叶绿体全基因组测序的成功促进了叶绿体分子生物学的研究,也为泡桐以及其他物种的遗传育种和创制种质资源奠定了一定的理论基础。     

生态有机肥不同混配基质对辣椒生长发育的影响

研究了“绿源”牌有机肥(生态有机肥)不同混配基质对辣椒生长发育的影响。结果表明:生态有机肥、蛭石、草炭按1:1:1或生态有机肥、蛭石按1:1组配明显促进辣椒的生长发育,主要表现为株高、茎粗增大,叶片数增多,光合作用增强,开花座果期提早,生物产量及经济产量提高。生态有机肥与河砂、炉渣组配有利于提高辣椒果实的Vc含量。全部以生态有机肥作为基质栽培辣椒效果较差。     

基于非线性降维时序遥感影像的作物分类

当前基于时序遥感数据的作物分类方法大都需要较多专家知识及人工干预,难以自动化,也难以移植到其他地区。将光谱降维技术用于时序遥感影像分析可以很好地解决这一问题。其中,非线性降维方法已经成功应用于高光谱数据,并且获得了比线性降维方法更好的结果。但是,直接将非线性降维方法用于时序遥感影像无法充分利用其时相维度的信息。该文改进了一种非线性降维算法——Laplacian Eigenmaps(LE)用于时序遥感影像的作物分类,该方法更加关注相同时相下不同作物生长季的物候特征差异,而不再仅依赖于整个生长季的物候曲线轮廓。改进的LE算法被应用于美国伊利诺伊州覆盖作物全生长季的Landsat 8时间序列影像。降维后保留的波段结合随机森林分类器基于美国农业部Cropland Data Layer(CDL)提供的训练数据完成了一系列的分类试验,并与传统插值未降维的方法进行对比。试验结果表明,改进的LE降维方法完成了更高的整体及各个类别的分类精度,其中整体分类精度达到85.37%,该方法作为一种自动化的方法,不需要人工干预,可直接移植到其他研究区,并且只需要较少的训练样本就可以完成一个较高的分类精度,为日后不同尺度的作物识别和提取研究提供了有效的方法。     

硼对瓜叶菊生长的影响

以不同浓度的硼喷施瓜叶菊叶面,探究硼对瓜叶菊生长发育的影响。结果表明:喷施一定浓度的硼能有效促进瓜叶菊叶片宽度的增大,植株的增高;瓜叶菊叶片内叶绿素、可溶性糖含量和硝酸还原酸活性的提高;瓜叶菊的千粒重及发芽率也有较大的提高;延长了瓜叶菊的花期和寿命。施用 0 05%浓度的硼综合效果最好。     

仁用杏开花座果的研究进展

仁用杏具有很强的经济、生态、社会效益,非常适合干旱半干旱地区恢复生态系统和发展农村经济。但仁用杏往往座果率低,产量不稳,影响仁用杏产业的发展。综合叙述了对仁用杏开花、座果规律的研究;从内因和外因方面揭示落花落果原因,特别是春季冻害对仁用杏落花的影响;并提出保花保果措施和方法．如水分和养分管理、树体管理、化学技术措施等。重点希望可以解决仁用杏的低温冻害等问题,为生产中提高仁用杏的座果率提供理论依据。