天麻田间高产栽培措施试验

为提高天麻栽培产量，扩大天麻栽培地域，提高山区有限土地利用率，笔者将影响天麻田间高产栽培的几个关键因子进行比较试验，总结出天麻田间高产栽培的有效措施，为大力发展天麻生产提供了科学依据。     

羊草草原枯枝落叶积累的研究：枯枝落叶积累对草原生态环境及生产力的作用

枯枝落叶积累可改变原生态环境，地表温度、土壤ＰＨ值积累量呈线性负相关，蒸发量、土壤容重与积累量呈指数负相关，土壤水分、土壤孔隙度与积累量呈线性正相关，枯枝落叶积累可为植物生长创造良好的生境。土壤中主要营养物质随着枯枝落叶积累均有明显提高当积累量达６００ｇ／ｍ＾２时，有机质、Ｎ、Ｐ、Ｋ含量分别比无枯枝落叶层提高１．８倍、８８．２％、２．１５倍和３７％。枯枝落叶积累对草原生产力有促进作用，在一定范围内     

Investigation of Cervical Patency and Uterine Appearance in Domestic Cats by Fluoroscopy and Scintigraphy

The cervical patency of six domestic female cats was monitored under sedation by infusion of contrast medium (Omnipaque) into the cranial vagina during early oestrus, mid‐oestrus, late oestrus and interoestrus or a radiopharmaceutical (^99mTc‐HSA) during mid‐ and interoestrus in a non‐ovulatory oestrous cycle. The transport of the contrast medium or the radiopharmaceutical through the cervix and within the uterine horns was observed under fluoroscopy and with the aid of scintigraphy. In three of the queens, transcervical transport of contrast medium was demonstrated in all stages of oestrus, in one queen during mid‐oestrus, late oestrus and 1 day after oestrus, and in two queens only during late oestrus. The relations between the cervical patency to the contrast medium and the oestrous behaviour, cornification of the vaginal cells and the serum oestradiol‐17β concentration were evaluated, and a relationship was found between the cervical patency and the degree of vaginal cornification. Transcervical transport of the radiopharmaceutical was observed in three queens during mid‐oestrus. When the cervix was open, hysterography under a fluoroscope and hysteroscintigraphy were performed. The fluoroscopic and scintigraphic recordings revealed the patterns of the uterine contractions during oestrus in both ascending and descending directions, and the movement of the uterine contents back and forth between the uterine horns. The hysterograms were classified according to the shape of the uterine horns and the appearance of the endometrial lining. Spiral‐shaped uterine horns with a smooth inner contour were observed in two queens, and a corkscrew appearance with irregular filling defects in the uterine lumen was shown in two queens that had developed subclinical cystic endometrial hyperplasia. These findings demonstrated that fluids or particles deposited in the cranial vagina of the cat can be transported into the uterus during some stages of the oestrous cycle. The fluoroscopic and scintigraphic techniques developed in this study may be further modified to permit more detailed studies of uterine contractile patterns and sperm transport in the feline female reproductive tract. Hysterography proved useful to diagnose uterine disease. The information on cervical patency is of value also for the development of techniques for artificial insemination in this species, and should be studied also in the ovulatory cycle.     

用分子生物学方法鉴别检测动物源性饲料中的牛羊源性成分

为了防止海绵状脑病疫区和痒病疫区反刍动物源性饲料进入我国 ,非常有必要对贸易往来中的肉骨粉品种来源进行鉴定检测。我们采用分子生物学方法从动物源性饲料中鉴别检测牛、羊特异性线粒体DNA的片段 ,并用限制性内切酶SspⅠ对PCR产物进行酶切鉴定及DNA序列测定。该方法的灵敏度可达 0 .12 5 % ,具有灵敏度高、快速和特异性强的优点。     

杂交水稻新组合从优9919特征特性及高产栽培技术

丛优9919是利用丛广41A为母本，与自育的新恢复系HR9919配组育成的高产较优质中迟熟籼型杂交稻新组合，2004年4月通过海南省品种审定，介绍了丛优9919主要特征特性及栽培技术要点．     

砼结构开裂原因分析及预防

该文简要说明了产生砼结构开裂原因及预防方法，分析了一些施工工艺技术引起的结构开裂因素及控制措施。     

Reproductive roles predict sexual dimorphism in internal and external morphology of lake whitefish,Coregonus clupeaformis

Abstract – The different reproductive roles of the sexes can predict the direction and magnitude of sexual dimorphism of external and internal morphology. Males should have enlarged structures that enhance the acquisition of mating opportunities, whereas females are predicted to have enlarged organs that are associated with the production of eggs. We tested these predictions in male and female lake whitefish, a species in which both sexes have similar overall body size and shape. After controlling for body size, male lake whitefish had significantly longer jaws and pectoral and pelvic fins, larger hearts, and more muscle than females. Sexual dimorphism in relative muscle mass may be one of the most fundamental morphological differences between males and females. Females had relatively heavier livers than males. Because the liver is important for the breakdown of fats and vitellogenesis, selection should favour an enlarged liver in females for the processing of energy and the production of large numbers of eggs.     

熵产生原理和耗散结构

本文讨论了熵产生原理和耗散结构理论的基本概念和有关问题,以及上述理论在生物学中的应用。     

实施结构调整发展马铃薯产业

毕节地区位于贵州省西北部，处于云贵高原台面与侧面的结合部，素有高寒山区之称。全区耕地面积43.3万hm2，其中，70%以上耕地分布在海拔1 400 m以上，旱地占86%，以旱地农业为主体。区内年均温12.8℃，降雨量1 000 mm左右，气候凉爽，雨量充沛，十分有利于马铃薯栽培。地区内马铃薯是主要粮食作物之一，播种面积和产量仅次于玉米位居第二，是山区人民的主副食品和发展畜牧业的主要饲料来源。因而马铃薯在毕节地区农业生产中占有举足轻重的地位。全区马铃薯播种面积1999年为14.3万hm2，占全省播种面积的1/3以上，是贵州马铃薯的主产区。就毕节地区得天独厚的地理、自然气候条件，将马铃薯产品生产开发建成一个大的产业是完全可能的。根据市场经济的发展，社会需求的变化，如何加快全区马铃薯产业的形成和发展，是当前急需研究和探讨的重要课题。 1 调整马铃薯的产业方向，明确马铃薯产业的发展目标 马铃薯的营养成分齐全、含量丰富，马铃薯及其产品的市场潜力很大，就毕节地区自然条件和市场需求看，毕节地区马铃薯产业的发展方向和目标可定位在三个方面。 1.1 种薯生产 毕节地区西部的赫章、威宁及中部大方的部分乡镇，气候冷凉，昼夜温差大，马铃薯生长茂盛、健壮，种薯质量好，历来都是本省和邻近省区的种源基地。但由于基地建设及栽培技术不规范，常以“大路货”作种薯调出，影响了市场的拓展。目前发展薯类已成为贵州省和南方许多省区种植业结构调整中的一个重点，抓住这个机遇，把毕节地区建成南方的一个种薯生产和供应基地是完全可能实现的。     

羊生长激素的提取及生物化学和生物活性的测定

600 g 冰冻羊垂体经过酸性溶液提取、硫酸铵的分级分离和 DEAE-纤维素柱层析得纯品(1),其得率为0.08g/kg,最后将部分纯品(1)过 Sephadex G-100分子筛,得到高纯的羊生长激素[纯品(2)]。提取的羊生长激素具有相当高的理化均一性,纯品(2):聚丙烯酰胺凝胶电泳呈现一条主带和二条酸性小带;聚丙烯酰胺等电聚焦电泳呈现二条带,其等电点分别为 pH7.35和 pH7.40,SDS—电泳呈现二条很难分开的带,其平均分子量为20 100道尔顿;用DNS-CI 法测得的 N-末端氨基酸是2个,丙氨酸和苯氨酸。纯品(1)理化均一性稍差:SDS—电泳较纯品(2)多出两条分子量低的带;等电聚焦电泳多出六条酸性小带。除此之外,其他理化性质同纯品(2)。用大白鼠胫骨实验测定生物活性,测得纯品(1)可使垂体大白鼠胫骨软骨盘的宽度相对增加79%。未对纯品(2)进行生物活性测定。用羊催乳素放射免疫药盒测定,纯品(1)和纯品(2)与羊催乳素均无交叉反应。