Silencing of TKTL1 gene by siRNA down-regulates HIF-1α expression and activity of glycolytic key enzymes in human cervical cancer cells

AIM：To explore the relationship between the expression of transketolase-like gene 1 (TKTL1) and glycolysis metabolism in human cervical cells. METHODS：The changes of hypoxia-inducible factor 1α (HIF-1α) expression and the activity of glycolytic key enzymes, hexokinase Ⅱ (HK-Ⅱ) and lactate dehydrogenase (LDH), under hypoxia in human cervical cell line HeLa were observed after TKTL1 was knockdown by siRNA. The specific siRNA expression vector targeting TKTL1 gene was constructed, and the recombinant plasmid was transfected into HeLa cells. The effects of TKTL1 silencing were evaluated by detecting transketolase (TKT) activity and TKTL1 mRNA expression using RT-PCR. The changes of HIF-1α expression and HK-Ⅱ expression, and HK-Ⅱ and LDH activity were also observed in transfected HeLa cells. RESULTS：The mRNA expression of TKTL1 and the activity of TKT decreased significantly (P<0.01) after TKTL1 silencing. Meanwhile, all HIF-1α expression, HK-Ⅱexpression, and HK-Ⅱ and LDH activity decreased significantly compared with the untransfected cells (P<0.01). CONCLUSION：Silencing of TKTL1 gene in human cervical cancer cells by siRNA down-regulates HIF-1α expression and the activity of glycolytic key enzymes, thus changing the malignant phenotype of carcinoma cells.     

不同果袋对‘蜜丝枣’果实品质及抗氧化酶活性的影响

以毛叶枣品种‘蜜丝枣’为试材,分析无色透明薄膜袋、白色无纺布袋、白色单层纸袋、外黄内黑双层纸袋4种果袋对‘蜜丝枣’果实品质及抗氧化酶POD、CAT活性的影响。结果表明:套袋能明显改善‘蜜丝枣’果实的外观色泽,提高果实抗氧化酶POD、CAT活性;套袋在一定程度上影响了‘蜜丝枣’果实的内在品质,降低了果实风味。套袋后,在4种果袋中,只有无色透明薄膜袋可以显著提高果实的单果重(比对照提高22.1%),而套其他3种果袋却会不同程度抑制果实生长,导致单果重不同程度下降;套无色透明薄膜袋对果实的内在品质影响程度最小,仅降低了可溶性固形物和可滴定酸,而对可溶性总糖无显著影响,对维生素C含量略有提高。综合套袋对果实品质和商品性的影响,推荐‘蜜丝枣’果实套袋材料为无色透明薄膜袋。     

1952—2017年出山店水库上游降水时空变化

为了探究出山店水库上游流域近65 a来降水的时空变化特征,基于出山店水库上游流域13个雨量站1952—2017年的逐日降水资料,运用气候倾向率法、Mann-Kendall趋势检验、Morlet小波分析和反距离权重IDW插值法等多种方法,研究了降水的年际变化、周期性与空间分布特征。结果表明:(1)流域1952—2017年多年平均降水量为1 028.3 mm,年降水量的气候倾向率为-20 mm/10 a(p<0.05),呈显著下降趋势,从季节上看,春、夏、冬降水随时间变化呈现减少趋势,秋季的降水呈现增加趋势;(2)年降水量呈现南高北低的空间分布,而年降水量的气候倾向率的变化趋势则与之相反;(3)流域降水量变化呈现20 a左右的第一主周期,同时流域的周期性变化也存在一定的空间分布特征。近65 a来,出山店水库上游年降水量变化趋势为先增加后减少,在整个时间域上有显著的下降趋势,并呈现20 a左右的周期性变化。     

Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek’s Diseases

To investigate the dynamic change of heat shock protein 90 （Hsp90） in the genesis and development of tumor, we successfully established tumor animal model using Marek’s disease and then determined the location of Hsp90 in the tumor tissue using immunohistochemistry method, the antibody titer level of Hsp90 in the serum and the expression level in the tissue using enzyme-linked immunosorbent assay （ELISA） method. Our result showed that Hsp90 location in the tumor tissue was signiifcantly associated with the tumor cell and most in the cytoplasm of the tumor cell, and Hsp90 expression level in the tissue and the antibody titer level in the serum was most signiifcantly increased with the development of tumor. This is the ifrst report to show the presence of Hsp90 in tumor tissues induced by the Marek’s disease, with its expression correlated to the tumoral grading. These data may also be valuable for developing new molecular anti-cancer therapies.     

Diversity of Antioxidant Content and Its Relationship to Grain Color and Morphological Characteristics in Winter Wheat Grains

The current interest in the health beneifts of whole wheat grain has prompted breeders to further increase the concentration of antioxidants in wheat. The objective of this study was to investigate the variation in antioxidant content among Chinese wheat grains and the relationship between antioxidants and grain color and morphological characteristics. A wide variation was observed in the total phenolic, carotenoid and lfavonoid contents, as well as the antioxidant activity （AOA）, of Chinese wheat varieties. Black wheat had the highest mean total phenolic, carotenoid and lfavonoid contents and the highest AOA, followed by red and white wheats. The grain color parameters were signiifcantly negatively correlated with total phenolic, carotenoid and lfavonoid contents and AOA among all of the wheat varieties examined, and grain weight was also signiifcantly negatively correlated with these traits. The same correlation between grain weight and antioxidant traits was also observed within individual groups of wheat, which indicates that grain weight may be used as an index for selecting wheat varieties with high AOA. Landraces had signiifcantly higher lfavonoid content than commercial wheat varieties. The results of this study may be useful for breeding nutrient-rich wheat varieties.     

吉林龙湾国家级自然保护区天牛昆虫的数值分类研究

以吉林龙湾国家级自然保护区12种常见的天牛为对象,测量其10种形态学指标,利用SPSS软件得到聚类树形图,从而对其亲缘关系进行分析,结果表明:1~3号为花天牛亚科;4~9号为沟胫天牛亚科;10、11号为天牛亚科;12号为锯天牛亚科,与传统的形态分类结果一致。     

油茶大田嫁接苗质量调查

对江西省16个1年生和10个2年生油茶苗圃进行调查,结果表明,1年生和2年生大田嫁接苗的平均合格率分别为48.0%和76.6%;2年生苗的合格率与土壤有机质和水解氮的含量正相关。2年生苗推荐的施肥水平为：土壤全氮0.114%～0.413%、水解氮114.86～132.92 mg/kg、全磷0.047%～0.073%、速效磷32.13～71.59 mg/kg、全钾1.203%～1.274%、速效钾48.08~67.08 mg/kg、有机质2.627%～3.171%。     

陇东苹果主要食心虫和卷叶蛾成虫发生动态监测

利用性诱剂对陇东地区梨小食心虫、李小食心虫和苹小卷叶蛾进行了成虫发生动态监测.结果表明：庆阳、平凉两市梨小食心虫、李小食心虫和苹小卷叶蛾均有发生,庆阳市较平凉市发生程度轻、发生晚、发生时间短、高峰次数少.     

半干旱区四种典型豆科牧草群落生理生态的分异研究

在黄土高原半干旱地区,植物种群生态的改善是植被建设与生态修复的关键。通过人工植被调查,比较了四种豆科牧草的种群生长的生态差异,初步探讨了牧草生产力与种群生态的可能耦合网络机制。结果表明:基于四种豆科牧草种群的土壤水分含量和垂直下降分布规律,发现沙打旺与苜蓿为高生产力型,红豆草与胡枝子为低生产力型,这可能与它们水分利用状况和豆科牧草本身的固氮或氮沉积的氮汇与源转化调节有关。苜蓿植株生物量表现较高,与其多年生庞大根系充分吸收土壤深层水分有关,反映了根冠生长与土壤条件的耦合是导致遗传特性响应差异的重要原因,因此调整植物根冠关系以增强植物的抗旱适应能力理论上是可行的。相关分析发现生产力、根系生物量、土壤环境(水分和层次)为网络耦联相关,特别是90-100cm的土壤空间水分与根冠比成正相关,这可能说明这个层次(根系的浅层集中分布区)为土壤水库与土壤干层相转变的阈值界面。因此从干旱胁迫条件下建立的适应性生存对策上考虑,苜蓿适宜在干旱立地推广应用,不仅具水土保持功效,而且植被生产力高,应为黄土高原生态恢复和生态建设的重要参考物种。