观赏植物养护专用缓释肥在3种植物上的应用研究初报

就一种新型观赏植物养护专用缓释肥在醉蝶花、罗汉松、莞香3种观赏植物上的应用效果进行初步探讨。结果表明:缓释肥可促进3种观赏植物的生长,与复合肥相比,该缓释肥具有肥效慢、肥效持续时间长、肥料利用效率高的特点。试验还发现,适量施用缓释肥可促进醉蝶花开花和延长开花期,且该缓释肥覆土施用要明显优于表土施用。     

Characters of the fibroblast-like cells cultured from the mobilized peripheral blood cells

AIM: To explore the characters of fibroblast-like (F-L) cells cultured from granunocyte clony stimunating factor (G-CSF)-mobilized peripheral blood cell (PBC) harvests. METHODS: The adherent cells in the PBC harvests were cultured for 2 week in the mediums of RPMI-1640/L-DMEM/G-CSF or interleukin-3 (IL-3) plus RPMI-1640, the cultured F-L cells were analyzed by flow cytometry (FC). RESULTS: The adherent non-confluent F-L cells obtained from the four groups were similar in their phenotypes: CD33+, CD11c+, CD64+, CD14+, CD45+, HLA-DR+, CD86+, CD34-, CD38-, CD3-, CD19-, CD56-, CD29-, CD44-, CD105-. The F-L cells are similar to monocytes except CD38- and were distinct from dendritic cells (DC) or mesenchymal stem cells (MSC). CONCLUSION: The cultured F-L cells are macrophages rather than DC or MSC. G-CSF, rhIL-3 enhances their numbers.     

乳鼠成骨细胞的培养及鉴定

无菌采取5日龄SD大鼠的颅盖骨,用胰蛋白酶预消化后,漂洗,剪碎,再用Ⅱ型胶原酶消化获得成骨细胞。通过倒置相差显微镜观察,并用碱性磷酸酶和矿化结节染色鉴定,利用微量滴定（MTT）法测定一个培养周期的成骨细胞活性分布,探讨一种经济、高效的原代成骨细胞培养方法。倒置相差显微镜形态观察结果显示,未贴壁的细胞呈圆形,贴壁生长的细胞呈不规则梭形、三角形或多角形,单核,有1-3个核仁;碱性磷酸酶和钙化结节染色均呈阳性（阳性率≥98.06%）;MTT法结合形态学观察结果显示,成骨细胞培养至第10 d时活性最强。证实用改良的二次酶消化法能够在短时间内获得大量成骨细胞,且所培养的细胞具有典型的成骨细胞形态和功能。     

Effects of different chemotherapeutic agents on reversing the acquired resistance to TRAIL gene in DLD1 colon cancer cells

AIM:To evaluate effects of different chemotherapeutic agents on reversing the acquired resistance to TRAIL gene and clarify the involved mechanisms in DLD1-TRAIL/R colon cancer cells. METHODS:Human colon cancer cell line DLD1-TRAIL/R cells that were resistant to TRAIL-expressing adenovector (Ad/gTRAIL) were treated with Ad/gTRAIL combined with different chemotherapeutic agents. Then, the cell viability was measured by MTT method, and apoptotic signaling conditions, including activation of caspase-3 and caspase-8, expression of Bax and Bcl-XL, were measured by Western blotting analysis. RESULTS:In vitro data showed that several chemotherapeutic agents, including 5-fluorouracil (5-FU) and mitomycin c (MMC), overcome the acquired resistance to TRAIL gene in DLD1-TRAIL/R colon cancer cells. The combination of Ad/gTRAIL and 5-FU effectively suppressed tumor growth in vivo in subcutaneous tumors established from DLD1-TRAIL/R cells. Further data showed that treatment with the combination of Ad/gTRAIL and 5-FU or MMC led to enhance the activation of caspase-3. Moreover, MMC but not 5-FU induced overexpression of Bax gene that was sufficient to overcome the resistance to TRAIL gene in DLD1-TRAIL/R cells. CONCLUSION:Chemotherapeutic agents, such as 5-FU and MMC, overcome the acquired resistance to TRAIL gene in DLD1-TRAIL/R cells. The candidate mechanisms for MMC but not 5-FU to overcome this resistance might involve the induction of over-expressed Bax protein in DLD1-TRAIL/R cells.     

不同覆盖方式下旱植水稻N、P、K吸收特性研究

以品种P88S/1128为材料,设置地膜覆盖、稻草覆盖和液体膜覆盖3个处理,以裸地为对照,研究了不同覆盖方式对旱作水稻N、P、K吸收特性的影响。结果表明,覆盖方式对旱作水稻N、P、K吸收特性有明显影响,稻草覆盖栽培有效提高了分蘖期植株对N素营养的吸收,为大群体的建立打下基础;成熟期茎秆和叶片中P素含量都降至最低,稻穗P素含量显著提高,其中以稻草覆盖处理最高,达3.70 g/kg,液膜覆盖处理次之,不覆膜最低;成熟期稻穗K素含量在成熟期降至最低,仅为2.40～3.08 g/kg,4种处理间差异不明显。从不同栽培模式比较来看,地膜、稻草、液膜覆盖处理对N、P、K营养元素的吸收量都显著高于不覆膜对照,表明覆盖栽培增强了旱植水稻对营养元素的吸收能力,其中以地膜覆盖处理最为明显。     

风景区自然景观资源评价及合理开发

文章以庐山风景区为例,对自然景观资源进行了分类与分级评价,并依据评价的结果及当前风景区自然景观资源的开发利用现状,提出了庐山风景区自然景观资源进一步科学开发与管理的合理化建议,以期为今后庐山风景区自然景观资源的可持续发展提供一定的理论参考。     

再生稻茎生腋芽的生育特性观察

为探索再生稻高产途径,观察了各节位茎生腋芽的分化、生长、穗粒发育性状和茎生器官形态.结果表明:茎生腋芽在母茎抽穗前按由下而上的节位顺序开始幼穗第一苞分化,在母茎抽穗后按由上而下的节位顺序进入一、二次枝梗分化;在顶端优势控制下,茎生腋芽至母茎黄熟前3 d才开始萌发,萌发成穗率随节位下移而降低,且生长先天不足,每穗粒数仅为母茎的三分之一.争取再生季高产的关键,是在头季收割保留上位优势芽的基础上,大力提高下部茎生腋芽的萌发率,形成比头季多70%～100%的穗数,以多穗补小穗的不足.倒3叶枕比倒2芽着生节部高8～16 cm,其高程可作为简捷诊断母茎适宜留桩高度的形态指标.     

桤木群体遗传分化研究I.DNA提取和PCR条件的建立

桤木(ＡｌｎｕｓｃｒｅｍａｓｔｏｇｙｎｅＢｕｒｋ)为桦木科(Ｂｅｔｕｌａｃｅａｅ)植物,主要分布于四川盆地及其周边地区。桤木喜光、喜湿,根系发达,对土壤要求不严,适应性强,在长江中下游地区防护林建设中具有重要作用。国外对桤木属(Ａｌｎｕｓ)植物的研究起步较早,主要集中于生理生化方面包括生长、固Ｎ、抗涝性以及种间杂交研究等[1　3]。虽有学者利用ＲＦＬＰ研究其核糖体ＤＮＡ的基因结构、以同工酶技术研究桤木属的群体遗传变异[4　6],但未见应用ＲＡＰＤ技术的研究报道。随着现代生物技术的发展,生物多样性的检测技术日趋成熟和多…