Analysis of the Relativity Between the Nutrient Content of Pasturage and the Relative Frequency of Feeding in Leizhou Goat Grazing on Natural Grassland

In order to understand the correlation between the grazing behavior of Leizhou goat and natural meadow nutrient content, the pasture behavior of Leizhou goats were tracked and recorded for 7 consecutive days, then the grass samples were collected according to the feeding conditions. The results showed that the relative feeding frequency of Pharbitis nil, Eleusine indica, Isachne globosa and Imperata cylindrica for Leizhou goats were 55.51%,28.70%, 8.13% and 1.18%,respectively.Among the determinated pastures, the contents of dry matter (DM)(P < 0.05),neutral detergent fiber (NDF)(P < 0.05) and acid detergent fiber (ADF) in Imperata cylindrica were the highest; Bidens pilosa had the highest contents of crude protein (CP)(P < 0.05),calcium (Ca)(P < 0.05),phosphorus (P)(P < 0.05), crude ash (ASH) and crude fat (EE);The crude fiber (CF) content in Isachne globosa was the highest. The correlation coefficient between the content of grass CP,CF,ADF,NDF,Ca as well as P and relative feeding frequency was 0.793(P < 0.05),-0.813(P < 0.05), -0.328(P > 0.05), -0.945(P < 0.01),0.804(P < 0.05),0.735(P < 0.05),respectively. In conclusion, there were significant positive correlation between the relative feeding frequency of Leizhou goats and the cotent of CP, Ca and P in native grass, and had a significant or extremely significant negtive correlation with CF and NDF content.     

人工饲粮对幼年工蜂生长发育及生理代谢的影响

本试验利用液相色谱-质谱联用技术(LC-MS)对幼年工蜂进行代谢组学分析,旨在探究不同人工饲粮对幼年工蜂生长发育及生理代谢的影响,为蜂王邮寄的人工饲粮配制提供一定理论依据.试验选取1日龄的意大利蜜蜂(Apis mellifera ligustica)300只,分为5组,分别饲喂5种人工饲粮,每组30只工蜂.对照组饲粮:...     

禽网状内皮组织增生症病毒检测方法研究进展

禽网状内皮组织增生症（Reticuloendotheliosis,RE）是危害养禽业的一种重要病毒性肿瘤病。本文概述了禽网状内皮组织增生症病毒（REV）实验室检测方法的研究进展。在细胞生物学方面,主要依靠病毒分离培养、病毒电镜形态检查、病毒特异性抗原及其抗体的检查等。在分子生物学方面,主要依靠国内外相继建立起的PCR、荧光定量PCR、LAMP技术及斑点分子杂交等。介绍和比较了各种方法的优缺点和实用性,为临床兽医科技工作者建立快速、简便、准确、实用的检测方法提供参考。     

钼对镉胁迫下山羊红细胞膜自由基代谢及脾脏组织病理学的影响

36只体质量约为20kg的山羊被随机分为4个组,每组9只,试验组灌服相同量的CdCl2和不同量的[（NH4）6Mo7·O24·4H2O],对照组灌服对应量去离子水。按每千克体质量计算,低钼组（0.5mg/kg（Cd）＋15mg/kg（Mo））、中钼组（0.5mg/kg（Cd）＋30mg/kg（Mo））、高钼组（0.5mg/kg（Cd）＋45mg/kg（Mo））,每组3个重复。于试验的第0、10、20、30、40、50天每组山羊采血,于试验的第0、25、50天每组山羊取脾脏和肝脏,测定相关指标。结果显示：（1）随着试验时间的延长,各试验组红细胞膜MDA含量呈上升趋势,GSH-Px、GST、SOD活性呈下降趋势;在第50天时,与对照组相比,各试验组红细胞膜MDA含量上升（P〈0.01）,GSH-Px、GST、SOD活性降低（P〈0.01）;（2）与对照组相比,高钼组脾脏的脾小体体积减小,可见散在的含铁血黄素沉积;（3）随着试验时间的延长,各试验组肝脏Bax基因表达量呈上升趋势;在第50天时,与对照组相比,低钼组和高钼组Bax表达量上升（P〈0.05）。结果表明,钼可导致镉胁迫下山羊红细胞膜自由基代谢紊乱,并能损害脾脏的免疫功能并且上调肝Bax基因的表达量,钼和镉体现为协同作用。     

鸽疱疹病毒PCR检测方法的建立及试剂盒的研制

鸽疱疹病毒(PiHV1)感染是由I型鸽疱疹病毒引起的以幼鸽为主的一种常见病毒性传染病,临床上以鼻炎、结膜炎为主要特征。偶尔也会出现精神沉郁、食欲下降、腹泻、呕吐、神经症状。根据已发表的鸽疱疹病毒的基因序列,设计并合成了一对能特异性扩增鸽疱疹病毒的引物,通过对PCR的退火条件的优化,建立了鸽疱疹病毒的PCR检测方法,对发病鸽子进行诊断。并研制出检测试剂盒,再进一步对该试剂盒的敏感性、特异性和稳定性进行了研究,证明该试剂盒具有很高的敏感性和特异性,并且在-20℃条件下可保存1年以上。     

EZH2-mediated regulation of NF-κB target gene expression in gastric cancer

AIM: To explore the mechanism by which over-expression of enhancer of zeste homolog 2 (EZH2) in a panel of gastric cancer cell lines is involved in tumorigenesis of gastric cancer. METHODS: Real-time PCR and Western blot were employed to examine the mRNA and protein levels of EZH2, respectively. MTS assay, cell migration and soft agar assay were performed to investigate the role of EZH2 in the regulation of stomach cancer behaviors. The effect of EZH2 on NF-κB target gene expression was determined by Luciferase reporter and real-time PCR. Co-immunoprecipitation was used to analyze the interaction of EZH2 and p65 in HEK293T cells. RESULTS: The expression levels of EZH2 were significantly increased in the gastric cancer cells compared with normal gastric epithelial cells. Pharmacological inhibition by DZNep or knockdown of EZH2 significantly compromised AGS and SNU-16 cell activity, cell migration and anchorage-independent cell growth. Moreover, siRNA knockdown of EZH2 impaired NF-κB downstream targets, such as IL-8, CXCL5 and CCL20. In addition, the interaction of EZH2 and p65 was detected. CONCLUSION: EZH2 mediates the growth of gastric cancer cells through the regulation of NF-κB downstream gene expression.     

抗寒梅切花优良品种选育研究

青岛梅园通过多年对梅花的研究实践,选育出4个可作切花且抗寒性强的优良梅品种,并就其选育技术进行了探讨。     

1766～1911年黄河中游汛期建立时间研究

利用清代奏报中保留的汛期涨水尺寸记录,近现代以来黄河中游水文站存在的水位信息器测数据,基于1766～1911年黄河中游万锦滩(位于今河南省三门峡市)水位志桩所载涨水时间,在"侯"尺度下建立了研究时段内三门峡断面的汛期开始时间。结果表明:1766～1911年,1840年代前中游汛期开始时间在平均水平;1840～1860年代汛期建立时间偏晚;1860～1890年代再次开始提前。在研究时段内黄河中游汛期开始时间平均为7月上旬,与同时期长江中下游梅雨结束时间有很好的对应关系,但年际差异较大,夏季风雨带推移成为导致中游汛期开始时间产生波动的主要驱动因素。     

外源花青素对干旱胁迫下烤烟幼苗生长及其生理特性的影响

以烤烟品种‘秦烟96’和‘云烟116’等2个烤烟品种为试验材料,通过开展盆栽试验,设置正常供水和中度干旱等2个水分梯度,外源花青素设置6个浓度,研究外源花青素对干旱胁迫下烤烟幼苗农艺性状、单株干重、光合气体参数、叶绿素荧光参数及活性氧代谢系统的影响。结果表明：干旱胁迫下,两个烤烟品种生长受到抑制且生理性能下降;干旱胁迫下喷施外源花青素可显著增加烤烟幼苗的株高、茎围、最大叶长、最大叶宽、最大叶面积、单株干重、净光合速率、气孔导度、蒸腾速率PSⅡ最大光化学效率、PSⅡ实际光化学效率和光化学猝灭系数,降低叶片中胞间CO₂浓度和非光化学猝灭系数,降低超氧阴离子自由基产生速率和丙二醛含量,提高叶片中超氧化物歧化酶、过氧化物酶和过氧化氢酶活性,增强烤烟幼苗对干旱胁迫的耐受性,有效缓解干旱胁迫对烤烟幼苗生长及其生理功能的损害;其中‘秦烟96’以叶面喷施3 mg·L^-1花青素对考烟幼苗生长的促进效果最佳,‘云烟116’以叶面喷施4 mg·L^-1花青素效果最佳。     

啶酰菌胺对水稻纹枯病菌的毒力及田间防效

采用菌丝生长速率法,在室内分别测定了啶酰菌胺(Boscalid)、井冈霉素(Jinggangmycin)和己唑醇(Hexacon-azole)对水稻纹枯病菌(Rhizoctonia solani Kühn)的毒力,并进行了田间防治试验。结果表明:啶酰菌胺、井冈霉素和己唑醇对水稻纹枯病菌菌丝生长抑制的EC50值分别为1.0692、96.2859和0.0240μg/mL;50%啶酰菌胺WDG 600倍液在第2次药后14 d对田间水稻纹枯病的防效为63.53%,极显著低于对照药剂20%井冈霉素A WP 1500倍液和5%己唑醇SC 600倍液的防效。