肉种鸡群晚开产的原因分析与启示

某种鸡场于2008年5月中旬引种AA+父母代肉种鸡12 600套,至29周才见蛋,且产蛋增幅缓慢,至34周产蛋率还不足30%,与相似文献   

肉鸽种蛋蛋重对孵化率和乳鸽生长发育的影响

以前,关于鸡蛋蛋重对雏鸡生长发育的影响有所报道[1],而关于种鸽蛋蛋重对乳鸽生长发育的影响研究较少,本试验旨在通过对美国落地王种鸽种蛋对孵化和生长发育影响的研究,进一步了解它们之间的关系.     

“黄白散”对兔接种RHD疫苗后免疫功能的影响

在注射兔病毒性出血症 (RHD)疫苗后 ,30只兔随机分为 2组 ,试验组兔饲喂含 1 5 %黄白散的饲料。从接种到第 1 80天 ,每组取兔 8只 ,每隔 1 5d采血 1次 ,检测血凝抑制 (HI)抗体 ;在接种前 1天及接种后 5、 1 0、 1 7、 2 4、 31、 41、 5 1d ,每组取兔 1 0只 ,采血检测ANAE+淋巴细胞百分率 ;在 1 80、 2 4 0、 30 0d时 ,每组分别取兔 5只做攻毒保护试验。结果表明 :2组兔HI抗体峰值分别为 7 3log2和 9 8log2 ,差异极显著 (P <0 0 1 ) ;试验组疫苗保护期可延长 60d ;试验组ANAE+率从第 1 0天至 5 1天与对照组比较 ,差异极显著(P<0 0 1 )。所以黄白散可增强兔接种RHD疫苗后的特异性免疫功能 ,增强RHD疫苗的免疫效果。     

牛体外受精时老化卵对受精率与染色体异常发生的影响

通过延长体外培养时间得到牛老化卵，对其体外受精率与染色体的异常发生进行了研究，体外受精48h后的受精率，46h培养组与22h和34h培养组之间差异显著（P＜0．05），随着老化时间的延长，受精率及胚胎发育速度明显降低。染色体分析结果表明，46h组的受精卵的染色体异常发生率为61．6％，22h组44．6％，2组之间差异显著（P＜0．05），单倍体（n=31)的发生率也随着老化时间的延长而增加，这些单倍体的性染色体的构成表明，含有X－性染色体的胚的出现率显著高于含有Y－染色体的胚的出现率（P＜0．05）。单倍体的发生是由卵的老化而引起单一配子的孤雌发生，体外培养时间的延长对正常的2倍体的性别无明显影响。     

杀菌型乳酸菌饮料的模糊数学综合感官评判方法

通过对市场上流行的5种杀菌型乳酸菌饮料的焦糖味、果味、发酵味、甜味、酸味、涩感的感官评定,采用组合赋权法得出杀菌型乳酸菌饮料焦糖味、果味、发酵味、甜味、酸味、涩感的质量权重.对5种杀菌型褐色乳酸菌饮料各指标以权重,然后结合模糊数学综合评判方法,得出模糊关系曲线.结果表明:5种杀菌型乳酸菌饮料的整体感官排序为:样品5＞样品2＞样品4=样品l＞样品3,与一般排序法所得结果样品2＞样品4=样品1＞样品5＞样品3相比较稍有不同.因此,模糊数学综合评判方法比常用的排序法更科学、合理、客观.     

西藏兔病毒性出血症的研究--病原鉴定及疫苗免疫效力试验

１９９４年６￣７月,西藏林芝地区家兔大批急性死亡同,经过流行病学调查,临诊断症状观察,病理剖检等结果与兔病毒性出血症特征相符。进一步作病毒学检查表明,病变组织超薄发切片经电子显微镜观察发现有大量直径约３０ｎｍ的球状裸听病毒粒子,自然病死兔肝组织匀交涉 清能凝集入）型红细胞,而且血凝兔出血症病毒阳性抗血清所抑制,致病性试验结果表明,该病毒对兔有很强的致病性;用西藏自然病死兔肝组织匀浆试制的矩形疫苗兔     

Vero E6细胞酵母双杂交cDNA文库的构建及鉴定

To seek out proteins which interact with structural proteins of porcine epidemic diarrhea virus （PEDV） in Vero E6 cells and study the mechanisms of viral infection.We created a Vero E6 cDNA yeast hybrid library. The total RNA of Vero E6 was extracted using Trizol method, and double strands cDNA was synthesized by SMART technique. We created a Vero E6 cDNA library by using homologous recombination in yeast. The results showed that the titer of cDNA library was 9.7×10⁸ CFU with the average of inserted fragments about 1.6 kb, and the recombination rate was 87%. These data indicated that the yeast two-hybrid cDNA library of Vero E6 was successfully constructed and might be useful for screening the host proteins interacting with structure protein of PEDV.     

Cloning and Sequence Analysis of Major Histocompatibility Complex Ⅰ Gene from Goose F1 of Fast-growth Lines

According to the multiple alignments identified major histocompatibility complex Ⅰ (MHC Ⅰ) gene conserved sequence registered in GenBank from the family ducks (Anatidae) anser waterfowl, a pairs of specific primers for the fragments of MHCⅠgene of goose F1 from fast-growth lines were designed and synthesized by Primer Premier 5.0. Using the genome DNA of goose F1 from fast-growth lines, the target gene fragment was obtained by PCR. To conduct sequencing of the fragments of MHCⅠgene of goose F1 from fast-growth lines and make sequence alignment and analysis of protein structure and function by bioinformatics, and research the characteristics of MHCⅠgene of goose F1 and the physicochemical properties of the protein. Bioinformatics was analyzed the nucleic acid data, deduced amino acid sequence and phylogenetic trees. The result of sequence analysis showed that the fragments of MHCⅠgene of goose F1 from fast-growth lines was 1036 bp in length, which coded 96 amino acids polyprotein. The homology were 93% and 83% with MHC Ⅰ gene and coding sequence of Wulong goose in NCBI respectively. There were 72 different bases sequence and 16 amino acids change. There also was higher homology with other poultry, and existed genetic relationship of Siji goose > chickens > ducks.The homology segment sequences corresponding to the fragments of MHCⅠ gene of goose F1 coded 96 amino acids protein, which molecular weight, PI, positively or negatively charged amino acid, estimated half-life, instability index, aliphatic index and average hydrophobicity were 11.342 ku, 5.32, 14, 17, 2.8 h, 34.92, 42.81, -1.066, respectively, and appeared 9 B cell epitopes, but contained no signal peptide. These results indicated that the protein for hydrophilic non-secreted proteins, had the high immunogenicity. In addition, The protein structure study indicated that alpha-helix, beta-sheet, beta-turn and random coil were 31.25%,16.67%, 14.58% and 37.50%, respectively. There existed amino terminal domain and carboxyl terminal domain in the tertiary structure. Therefore, MHC gene had significant difference between species and populations of individuals by the pathogen pressure in environment, and there were the interaction between polymorphism of MHC molecules and the diversity of antigenic peptide. MHC determined the differences of individual susceptibility to disease, and could be treated as a candidate gene for disease resistance.     

中度盐碱化草地混播牧草替代玉米青贮对绵羊生产性能的影响

为研究中度盐碱化草地混播牧草对绵羊采食量、日增重和屠宰性能的影响,试验选用24只3月龄、体重(23.1±0.62) kg的德国肉用美利奴杂交一代绵羊,随机分为4组,对照组饲喂精料补充料十玉米(Zea mays)青贮,处理Ⅰ,Ⅱ和Ⅲ组在对照日粮基础上分别以中度盐碱化草地混播牧草(披碱草(Elymus dahuricus)、碱茅(Puccinellia distans)和沙打旺(Astragalus adsurgens))替代1/3,2/3和3/3玉米青贮,每只羊精料补充料日喂600 g,粗饲料自由采食.结果表明:处理Ⅰ,Ⅱ和Ⅲ组的有机物、干物质、酸性洗涤纤维、中性洗涤纤维采食量均显著高于对照组(P＜0.05);处理Ⅰ和Ⅱ组的日增重虽略高于对照组,但差异不显著;处理Ⅱ和Ⅲ组的饲料转化率显著高于对照组;各处理组绵羊屠宰体重、胴体重、屠宰率、净肉率、胴体出肉率、肉骨比、眼肌面积均较对照显著提高(P＜0.05).因此,中度盐碱化草地混播牧草替代玉米青贮可以增加绵羊的采食量,提高绵羊的屠宰性能,并以处理Ⅰ和Ⅱ组的效果较理想.     

24％溴虫腈·辛硫磷乳油对家蚕残毒试验

桑园治虫用药一直移用农田农药,安全性差,家蚕中毒事故频发;近年虽有一些桑园专用农药推出,但由于数量少,使用频率高,促成了害虫耐药性迅速提高,造成多种药物防效明显下降;开发桑园专用新农药,对桑园害虫控制和抗性治理都有积极意义。