乳胶凝集试验与血清中和试验检测猪伪狂犬病血清抗体的 …

应用血清中和试验（ＳＮＴ）和伪狂犬病乳胶凝集试验（ＬＡＴ）诊断试剂盒对两种伪狂犬病是性血清、伪狂犬病病毒（ＰＲＶ）高兔血甭及６０份被检猪血清进行了ＰＲＶ抗体效价测定和相关性分析,两种方法测得的抗体效价之间呈强相关性（ｒ＝０．９６）,且ＬＡＴ效价比ＳＮＴ一般高出一个滴度;能干为自３５个猪场的４１４份猪血清进行了ＰＲＶ抗体检测,并与ＳＮＴ检测结果进行了对比,结果在ＳＮＴ检测为阳笥的１７１份血清中,ＬＡ     

DNA Barcoding:An Effective Tool for Species Identification of Lepidopteran Oak Pests

[Objective] The paper was to improve the efficiency and accuracy of early forecast of Lepidopteran oak-infesting pests.[Method] DNA barcoding technique was established for quick species identification using mitochondrial cytochrome C oxidase subunit Ⅰ(COⅠ) as the standard gene.This barcoding technique was used to amplify and sequence genomic DNA samples from eggs and pupae of 11 species of Lepidopteran pests collected from oak.[Result] The DNA barcoding standard genes of 594-708 bp were determined from eggs and pupae of Lepidopteran insects.There were differences of 0-2 bases in DNA barcode sequences between conspecific eggs and pupae,with the sequence identity of 99.7%-100%.The average content of A,T,G and C of DNA barcode sequences from Lepidopteran insects were 30.7%,38.5%,14.9% and 15.9%,respectively.The obtained DNA barcode sequences had 91.4%-100% identity and 0-8.6% difference degree with GenBank-deposited DNA barcode sequences from organisms of the genetically-closest relationship.Among them,DNA barcode sequences from egg and pupa samples of 10 Lepidopteran insects(No.1-20) had 99%-100% identity and 0-1.0% difference degree with homologous sequences in GenBank database,while the remaining samples(No.21-22) had high difference degree(8.6%) with homologous sequences.[Conclusion] The established DNA barcoding technique is an effeetive tool for species identification of Lepidopteran pests using genomic DNA from eggs and pupae of Lepidopteran insects.     

肌肽活性的生化基础及其应用

肌肽是由β-丙氨酸和L-组氨酸组成天然活性二肽,可螫合过渡金属离子,具有抗氧化及抗衰老等生物学活性,其游离氨基羧基及咪唑氮可有效接纳质子或羟基,稳定溶液pH等。就肌肽活性的生化基础及应用研究作一综述。     

不同生长期金荞麦营养成分含量及消化率测定研究

为系统地测定分析不同生长期金荞麦的常规营养成分、氨基酸、微量元素含量及其回肠末端消化率, 本研究用3头安装“T”型瘘管, 并做回-直肠吻合切除盲肠的巴克夏-驯养野猪-高坡黑猪(巴-野-高)三元杂交生长猪作为试验动物, 采用3×3拉丁方试验设计开展消化试验。结果表明, 分枝期、孕蕾期和初花期金荞麦OM、CP、EE、Ca、P、CF含量分别为88.59%、22.72%、2.34%、1.05%、0.39%、13.51%, 89.10%、20.57%、1.69%、1.25%、0.42%、15.50%和89.63%、17.54%、1.37%、1.29%、0.46%、19.75%;EAA、NEAA和TAA含量分别为9285、7982、6244 mg/100 g, 14334、10810、9320 mg/100 g和23619、18792、15564 mg/100 g。随着生长期的后延, 营养成分含量显著降低, 在体和离体消化率也随着生长期的后延而显著降低。分枝期与孕蕾期间营养成分含量差异显著(P<0.05), 分枝期与初花期间营养成分含量差异极显著(P<0.01), 孕蕾期与初花期间营养成分含量差异显著(P<0.05)。从分枝期到孕蕾期, 金荞麦CP、EE、EAA及TAA下降较慢, CF含量升高较慢, 营养成分消化率下降也较慢;从孕蕾期到初花期, 金荞麦CP、EE、EAA及TAA下降较快, CF含量升高也较快, 营养成分消化率下降也较快。以分枝期、孕蕾期和初花期体外法测定常规营养成分及微量元素消化率值为自变量, 以对应的动物饲养试验回肠末端表观消化率值为因变量建立的回归方程拟合度好,可用于推算对应的回肠末端表观消化率。本实验证明, 金荞麦营养丰富、消化率高, 必需氨基酸比例高、适合动物消化吸收。孕蕾期为金荞麦适宜收割期, 在适宜收割期内, 收割时间点应选择孕蕾后期。     

县级畜禽屠宰管理工作中的新问题剖析

江西省南丰县畜禽屠宰管理职责由商务局移交农业部门后,相关工作进入一个全新的模式。但制度设计、单位状况、社会习惯等方面因素影响了交接后畜禽屠宰管理工作稳步发展。对于南丰县畜禽屠宰管理工作,笔者认为应从坚持政府主导,充分发挥市场的积极作用,大力查办违法违规案件及强化部门间协调配合等方面入手加以解决。     

基于TM和ASTER的植被指数建模性能对比

在对TM、ASTER数据进行多种植被指数生成的基础上,结合研究区48个杨树样地的测树因子数据,分别就杨树单位面积蓄积量、杨树平均高和杨树年龄进行了遥感统计建模研究,通过植被指数及其变形式共65个特征对比结果表明,由ASTER数据派生的植被指数中RVI3(0.5)对于上述3个测树因子的估测性能最好,由TM数据导出的植被指数中则是DVI2对上述3个测树因子回归效果最好,从建模性能上分析,ASTER数据生成的植被指数要优于TM数据生成的植被指数。     

一氧化氮信号途径参与草地早熟禾耐镉机制的研究

为探究信号分子一氧化氮（Nitric Oxide,NO）对镉（Cadmium,Cd）胁迫下草地早熟禾（Poa pratensis）的调控机理,揭示草地早熟禾耐镉机理,本试验采取根施1000 μmol·L^-1氯化镉（Cadmium chloride hemidihydrate,CdCl₂·2.5H₂O）及叶面喷施NO合成酶抑制剂（L-硝基精氨酸甲酯（L-NAME））、硝酸还原酶抑制剂（钨酸钠（Tungstate））、NO清除剂（4-羧基苯-4,4,5,5-四甲基咪唑-1-氧-3-氧化物（cPTIO））处理草地早熟禾叶片,研究不同处理对草地早熟禾幼苗叶片生长及抗氧化酶活性、光合色素含量、丙二醛（Malondialdehyde,MDA）、游离脯氨酸、内源NO含量的影响。研究发现,镉胁迫下草地早熟禾内源NO含量增加,添加一氧化氮清除剂、一氧化氮合酶抑制剂、硝酸还原酶抑制剂可增加超氧化物歧化酶（Superoxide Dismutase,SOD）、过氧化氢酶（Catalase,CAT）、抗坏血酸过氧化物酶（Ascorbate peroxidase,APX）活性,降低过氧化物酶（Peroxidase,POD）活性,增加叶绿素a、叶绿素b、类胡萝卜素、丙二醛含量,降低脯氨酸（Proline,Pro）含量、叶片相对含水量（Relative Water Content,RWC）和干物质量（Dry matter content）。结果表明,一氧化氮合酶途径可能是早熟禾合成NO的主要途径,内源NO可通过调节抗氧化酶活性、光合色素含量、游离脯氨酸含量等途径缓解镉胁迫。     

A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci-infected domestic poultry in Zhejiang Province,China

A cluster of Chlamydia psittaci (C. psittaci) cases was reported in Zhejiang Province, China, 2019. This study evaluates the extent of the outbreak and determines the source of infection. Real-time PCR and sequencing of the ompA gene of C. psittaci were performed to identify the cases, the domesticated poultry and close contacts. The index patient was a 76-year-old woman with chronic vertigo, and Case 2 was a 64-year-old female farmer with herpes zoster. Both women bought psittaci-infected chickens or ducks from the same mobile street vendor and raised them for 10 days and 23 days before fever onset. There were no direct contact between the two women. C. psittaci test was positive for the two patients, one sick chicken, three healthy ducks and the vendor's chicken cage. Phylogenetic analysis showed that all seven C. psittaci positive samples carried identical ompA genotype A of C. psittaci. Of all of the patients' 148 close contacts, none tested positive for C. psittaci, or developed acute respiratory symptoms. Both patients were discharged after a 4-week hospital stay. In conclusion, the source of this cluster was the poultry infected with C. psittaci, which occasionally cause infections in farmers, but inter-human transmission seems unlikely.     

四川省毒害植物——紫茎泽兰调查报告

紫茎泽兰是恶性毒害植物,已传入攀枝花市和凉山州两地１４县３区１市,分布面积３７５１６０ｈｍ２,每年以２０～３０ｋｍ的速度向北向东传播。其繁殖能力和侵占性极强,是农、林、牧业生产的大敌,应采取切实可行的方法,发动群众,人人动手,把防除与开发利用结合起来,开展综合防治。     

三种鹅鸡草的生物系统学研究

通过对鹅观草属犬草组长芒鹅观草、缘毛鹅观和鹅观草３个种及春种间杂种的形态变异、染色体配对行为和繁育特性进行研究,探讨这３个种的亲缘关系。Ｆ１植株的外形趋于两亲本之间。在减数分裂过程中,亲本种染色体配对正常。杂种Ｆ１的染色体配对数较高,平均构型：Ｒ．ｐｅｎｄｕｌｉｎａ＊Ｒ．ｄｏｌｉｃｈａｔｈｅｒａ为０．５６１Ⅰ＋１３．７２Ⅱ;Ｒ．ｄｏｌｉｃｈａｔｈｅｒａ＊Ｒ．ｋａｍｏｊｉ为７．５８Ⅰ＋１３．５８Ⅱ＋