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911.
周莲云 《农业图书情报学刊》2013,(12):89-91
开展读书活动是提高全民族科学文化水平的最佳途径.图书馆作为精神文明财富的社会公益性文化设施,要充分发挥自身职能,积极开展读书活动,努力创建行之有效的活动模式,掀起全社会的读书热潮,在精神文明建设中发挥其不可替代的作用. 相似文献
912.
在介绍宁夏现代化农产品物流体系存在的问题的基础上,剖析了产生问题的原因,提出了相应的对策。 相似文献
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生物炭对植烟土壤养分的影响 总被引:4,自引:0,他引:4
为探讨生物炭作为土壤改良剂对植烟土壤养分含量的影响,以湖南烟区主要栽培品种云烟87为试验材料,通过大田试验研究了不同的生物炭用量(CK:0 kg/hm~2;T1:3 000 kg/hm~2;T2:3 750 kg/hm~2;T3:4 500kg/hm~2)下植烟土壤中的养分含量。结果表明:施用生物炭能够显著提高植烟土壤有机质、碱解氮、铵态氮及速效钾含量,且随着生物炭施用量的增加呈先升高后降低的趋势,从改良土壤肥力效果来看,生物炭施用量在3 750 kg/hm~2的水平下较为适宜。 相似文献
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919.
以同一棵毛竹笋上快速伸长生长的节间组织和基本停止伸长生长的节间组织为材料,分别提取总RNA,纯化成mR-NA后合成cDNA双链;以Uni-ZAP XR vector为载体,构建了2个cDNA文库。快速伸长生长的、停止伸长生长的节间组织cDNA文库的滴度分别为9.7×109、8.4×109pfu.mL-1,含插入片段的频率均达到99%以上,插入片段的大小均在500~3000 bp。cDNA文库的成功构建为探究毛竹快速生长的分子机理奠定了基础。 相似文献
920.
LUO Dan SHU Sai-nan ZHOU Yu-feng LIU Xing-lou TIAN Jia WANG Hui ZHOU Hua LI Ge FANG Feng 《园艺学报》2009,25(11):2173-2181
AIM: The influence of MCMV infection on differentiation and differentiation gene expression in neural stem cells(NSCs) in vitro were investigated for studying the mechanisms of brain abnormalities caused by congenital cytomegalovirus infection.METHODS: NSCs were separated from fetal BALB/C mouse, and cultured and identified in vitro. The differentiation potency of NSCs was observed by immunofluorescence. The NSCs infected by MCMV at dosage of MOI(multiplicity of infection) equaled to 5, 1 and 0.1,respectively, were cultured in differentiation medium. The morphological changes of infected cells were observed under inverted microscope. The ratios of NSCs and its differentiated cells were detected by flow cytometry. The expressions of nestin, GFAP and NSE, markers of NSCs and its differentiated cells, were studied by immunofluorescence(MOI=1). The expression of early antigen(EA) of MCMV was detected to observe the infection process. Real-time RT-PCR method was employed to measure the expression levels of the key genes Neurog2, Myc and Ccnd1 in Wnt signal pathway of NSCs at early stage of differentiation culture.RESULTS: NSCs isolated from embryonic mouse brains proliferated to form neurospheres, strongly expressed nestin and differentiated into NF-200 positive neurons or GFAP positive astrocytes. The infected NSCs did not adhere to the wall and appeared differentiation growths, but showed swollen gradually after differentiation culture. The nestin expression in the infected cells downregulated slowly and was higher than that in control groups(P<0.05). The GFAP and NSE expressions of the infected cells were lower than those in control groups(P<0.05). The early antigen(EA) of MCMV was always detected in the cells in infected groups. The ratios of nestin positive cells in infected groups were higher than those in control groups, but the ratios of GFAP and NSE positive cells of former were lower than that of the latter from 3rd to 9th d after differentiation culture(P<0.05). The levels of Neurog2 mRNA and Myc mRNA in infected groups were markedly lower than those in normal control groups on 1st d and from 1st to 4th d after differentiation culture, respectively(P<0.05). The levels of Ccnd1 mRNA of infected groups were obviously lower than those in normal control groups from 12th h to 1st d(P<0.05). These changes in infected groups became more obvious as MCMV MOI increased.CONCLUSION: MCMV significantly inhibits differentiation of NSCs to neurons and astrocytes, and leads to the decrease in differentiated cells. MCMV inhibits or interferes with the gene expression of Neurog2, Myc and Ccnd1 in Wnt signal pathway of NSCs. The effect that MCMV inhibits the expressions of differentiation and the differentiation genes in NSCs shows dose-dependent with MCMV MOI. The inhibitory effect of MCMV on the differentiation of NSCs might be induced by interfering with the expression of differentiation gene in NSCs, which is possibly the one of primary causes of brain development disorders induced by congenital CMV infection. 相似文献