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11.
OUYANG Jing-ping LIU Yong-ming ZHENG Han-qiao WEI Lei TU Shu-zhen YANG Hai-lu CUI Ye-jian 《园艺学报》2000,16(1):70-73
AIM: The effects of BDM on isolated rat heart in cold cardioplegia were studied. METHODS: Rat heart were subjected to cold cardioplegia at 4℃ for 8, 18 and 24 h.Then each heart was perfused (90 cm H2O) in Langendorff model at 37℃ for 40 min. In the high K+ group(n=24) the hearts were preserved in St.Thomas cardioplegic solution, in BDM group(n=24) hearts were preserved in K-H solution with BDM 30 mmoL/L. RESULTS: After 18 h, heart rate and the coronary flow in BDM group were significantly higher than in high K+ group(P<0.05). Activity of Na+-K+-ATPase in cell membrane and in mitochondrial membrane in the BDM group was significantly higher than high K+ group(P<0.01). After 24 h, all hearts in high K+ group were dead, but were alive in BDM group. CONCLUSION: Under given experiment conditions, BDM did enhance the tolerance to cold ischemia significantly. The results showed that BDM may become a useful agent for prolong the storage period of heart in cold cardioglegia. 相似文献
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CHEN Yu-lu WANG Xin-yu ZHENG Shu-zhan DU Yan-fei YE Qiang LI Jia-fu FAN Zhong-cai LI Guang 《园艺学报》2000,36(8):1531-1536
AIM To observe the effect of recombinant mouse interleukin-11 (rmIL-11)injected subcutaneously into mice on heart structure and function and to determine its pro-fibrotic effect. METHODS C57BL/6 mice were randomly divided into experimental group and control group.The mice in experimental group were injected subcutaneously with recombinant mouse IL-11 at the dose of 100 μg·kg-1·d-1 for 3 consecutive weeks, while the control group were given equal volume of normal saline in the same way. After the experiment was finished, the parameters of heart function were measured by echocardiography.The heart weight was weighed and the cardiac weight index (CWI) was calculated. HE staining and Masson's trichrome staining were performed to observe the pathological changes and the extent of myocardial fibrosis in mouse myocardia respectively, and the cardiac collagen volume fraction (CVF) was calculated. The expression levels of extracellular matrix proteins in the myocardial tissues of mice, including type Ⅰ collagen, type Ⅲ collagen and fibronectin, were determined by Western blot. RESULTS Left ventricular ejection fraction and left ventricular fraction shortening in experimental group were obviously lower than those in control group (P <0.01), however left ventricular end-diastolic diamension and left ventricular end systolic dimension were significantly higher than those in control group (P <0.05).Compared with control group, the CWI was increased (P <0.01), the myocardial arrangement was disorder, the necrosis of cardiac myocytes was increased, and excessive deposition of collagen was observed in the myocardial tissues in experimental group. Correspondingly, the CVF and protein levels of type Ⅰ collagen, type Ⅲ collagen and fibronectin in the left ventricle in experimental group were increased significantly (P <0.05). CONCLUSION Injection of rmIL-11 into the mice subcutaneously induces fibrogenesis in the heart, which implies that IL-11 is likely a novel pro-fibrotic factor. 相似文献
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利用4 434个SNP标记对47份玉米自交系进行种质遗传多样性和杂种优势类群研究。采用Admixture软件对自交系的遗传结构进行分析,采用Treebest软件对自交系进行聚类分析和类群划分。结果表明,47份自交系分为6个类群,分别为瑞德群、兰卡斯特群、塘四平头群、PB群、旅大红骨群和自330亚群。10个自选系组配的杂交种主要利用的杂种优势模式为PB×塘四平头和瑞德×塘四平头,均为近年来黄淮海地区主要利用的杂种优势模式。 相似文献
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集雨农业是指收集、存储雨水径流并将其作为农村家庭人畜饮用水、环境卫生用水和农业补充灌溉用水的农业生产活动,包括收集、存储、输送、利用等多个过程,但目前各地的集雨农业技术研究多注重单项技术研究,而没有把它们当作一个整体来进行研究,导致许多地方出现收集与存储、收集与利用、存储与输送、存储与利用等脱节的现象,使各个环节的雨水利用转化效率普遍偏低。本文首先比较系统完整地提出了集雨农业雨水利用转化效率的概念,将集雨农业的雨水收集、雨水存储、雨水输送和雨水利用等各个过程看作一个整体进行研究分析,然后结合有关试验资料提出了提高集雨农业雨水利用转化效率的途径。 相似文献
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为探究蚯蚓粪与化肥配施对陕西关中地区红小豆产量及品质的影响,以红小豆新品种保红947为研究对象,设置T1(纯化肥)、T2(20%蚯蚓粪+80%化肥)、T3(50%蚯蚓粪+50%化肥)、T4(80%蚯蚓粪+20%化肥)和CK(不施肥)5个处理,研究了不同处理下红小豆成熟期农艺性状、产量性状及品质性状的差异.结果表明:蚯蚓... 相似文献
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从水资源产权的角度来理解绿洲生态用水,期望为绿洲生态和环境的保护与恢复提供新的研究思路。首先对生态用水概念进行了辨析,给出了绿洲生态用水的概念;其次对绿洲生态水权从质和量两个方面进行了界定;最后提出绿洲生态水权分配的层次结构,介绍了绿洲生态用水的计算方法,重点对绿洲生态水权进行分配。从而进一步明晰了绿洲生态水权,为绿洲生态与环境的保护和恢复提供了新的理念和思路。 相似文献
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HUANG Yi HU Jian-da ZHENG Jing LI Jing WEI Tian-nan ZHENG Zhi-hong CHEN Ying-yu 《园艺学报》2012,(1):70-75
AIM: To study the effects of baicalin on CA46 cell xenografts in nude mice. METHODS: The nude mice with CA46 cell xenografts were treated with drugs via intraperitoneal injection daily, and were divided into 5 groups: negative control group, 15 mg/kg baicalin group, 30 mg/kg baicalin group, 60 mg/kg baicalin group and 4 mg/kg etoposide (VP-16) positive control group. After 12-day treatment, the weight of CA46 cell xenografts stripped from some nude mice in the 5 groups was used to evaluate the effect of baicalin on xenograft growth in the nude mice. The apoptosis, necrosis and pathological changes of the xenograft cells were examined under light microscope and transmission electronic microscope respectively. The expression levels of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway-related proteins extracted from xenografts were determined by Western blotting. The other nude mice with CA46 cell xenografts in the 5 groups continued to be treated with the drugs until death in order to evaluate the effect of balcalin on survival time of the nude mice with CA46 cell xenografts. RESULTS: Baicalin remarkably inhibited the growth of CA46 cell xenografts, induced apoptosis and necrosis of xenograft cells, and reduced the protein expression of phospho-Akt (p-Akt), nuclear factor-kappa B (NF-κB), mammalian target of rapamycin (mTOR) and phospho-mTOR (p-mTOR) in the xenografts after 12-day treatment. Furthermore, baicalin prolonged the survival time of the nude mice with CA46 cell xenografts in a dose-dependent manner. CONCLUSION: Baicalin inhibits the growth and induces apoptosis of CA46 cell xenografts in the nude mice, and prolongs the survival time of the nude mice with CA46 cell xenografts through the mechanism of down-regulating PI3K/Akt/NF-κB and PI3K/Akt/ mTOR signaling pathways. 相似文献