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101.
AIM: To explore the effect of EBV infection on growth and apoptosis of nasopharyngeal carcinoma(NPC) cell line.METHODS: NPC cell line CNE1 was directly infected by Epstein Barr virus (EBV). The expression of EBV-latent membrane protein 1 (EBV-LMP1) and bcl-2 were detected by immunohistochemistry method (LSAB). The growth of NPC cells was identified by MTT method. Apoptotic carcinoma cells were detected by flow cytometry analysis and the terminal deoxynucletidyl transferase-medicated dUTP-biotin nick end labeling (TUNEL) methods. RESULTS: EBV-LMP1 was positive in CNE1 infected by EBV(E-CNE1). Compared with CEN1, the growth of E-CNE1 apparently increased (P<0.01). No apoptotic carcinoma cells were detected and bcl-2 postive cells were 2%~3% respectively in 2 kinds of NPC cells.CONCLUSION: Growth of NPC cells is enhanced by EBV infection and EBV-LMP1 expression, but no influence on expression of bcl-2 and apoptosis of NPC cells. 相似文献
102.
YAN Xiao-cheng MU Wei-na QIANG Ye ZHAO Hui-chen SUN Qi YAO Xiao-min ZHANG Yu-chao LIU Yuan-tao 《园艺学报》2000,36(9):1661-1666
AIM To investigate the effects of cytochrome P450 (CYP450) epoxygenase/epoxyeicosatrienoic acid (EET) pathway on insulin resistance in obese mice, and to explore the possible mechanisms. METHODS High-fat diet-induced obesity model was established in C57BL/6Cnc mice, and the obese mice were randomly divided into 3 groups, including obesity group (treated with saline; n =10), EET group (treated with 11,12-EET; n =10) and EET inhibitor 14,15-epoxyeicosa-5(Z)-enoic acid (EEZE) group (n =10). Normal C57BL/6Cnc mice (n =10) treated with saline served as control. Protein expression of CYP2J2 (one of CYP450 epoxygenases) and hypoxia-inducible factor-1α (HIF-1α) was measured by Western blot. Vessel-like structure was detected by immunofluorescence staining. The serum levels of insulin, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and monocyte chemoattractant protein-1 (MCP-1) were measured by ELISA. RESULTS In obese mice, homeostasis model assessment of insulin resistance (HOMA-IR) values were increased, the protein level of CYP2J2 was reduced, and the protein level of HIF-1α was increased in adipose tissues as compared with the controls (P <0.05). The serum levels of MCP-1, IL-1β, IL-6 and TNF-α were also significantly increased in obese mice (P <0.05). After treatment with 11, 12-EET, the HOMA-IR values were decreased compared with vehicle-treated obese mice, HIF-1α expression levels were decreased in the adipose tissue, and the serum levels of MCP-1, IL-1β, IL-6 and TNF-α were reduced (P <0.05). Immunohistochemical results of adipose tissue from vehicle-treated obese mice showed a marked decrease in vessel-like structures (CD31-positive) compared with normal control mice (P <0.05). EET treatment significantly increased the newly formed vessel-like structures in the visceral adipose tissues of obese mice as compared with vehicle-treated obese mice (P <0.05). CONCLUSION High-fat diet-induced obesity and insulin resistance are closely related to the CYP450 pathway. Exogenous EETs effectively decrease obesity-induced insulin resistance possibly through pro-angiogenesis and attenuation of hypoxia and inflammation. 相似文献
103.
GUAN Cang-hai ZHAO Yu-qiao Guo Liang CHEN Yu-Zhu WANG Wei-Na JIANG Xing-ming 《园艺学报》2000,36(10):1908-1912
MNX1 antisense RNA 1 (MNX1-AS1) is a newly discovered long non-coding RNA (lncRNA), which is highly dysregulated in various carcinomas and its expression level is closely related to the overall survival and prognosis of patients. MNX1-AS1 regulates the occurrence and development of carcinomas by endogenous competitive adsorption of miRNA, regulating cell cycle, inducing epithelial mesenchymal transformation and activating multiple signaling pathways. The in-depth study of the carcinogenesis of MNX1-AS1 is useful for the early diagnosis, targeted therapy and prognostic assessment of relevant carcinomas. This article reviews the roles of MNX1-AS1 in malignant tumor. 相似文献
104.
105.
以200份玉米优良自交系为材料,2022-2023年夏分别进行小斑病菌的田间接种,综合两年数据共鉴定出高抗小斑病的玉米自交系3份,分别为K22、CML50和齐319;筛选到包括Mo17在内的抗性玉米自交系24份。选取K22和B73两个具有不同抗性水平的玉米自交系进行小斑病菌的室内接种。结果表明,48 h和72 h时,自交系K22叶片的病斑数、病斑面积和病斑面积比等各项指标均显著优于自交系B73,进一步验证田间鉴定的准确性。48 h或72 h时,高抗小斑病自交系K22与感病对照B73相比,5种防御酶活性增加14.32%~70.45%。 相似文献
106.
基于2019-2022年夏玉米播期试验资料,分析夏玉米生理成熟后延迟收获对子粒农艺性状改善、增产效应。结果表明,早播10 d至晚播20 d处理的有效灌浆结束日均比生理成熟期延后,子粒有效灌浆持续日数依次比其花后10 d至生理成熟期日数延长28~9 d,播期每推迟10 d延长日数减少6.3 d。子粒农艺性状有明显的改善,百粒重、穗粒重提高,子粒增产效应明显,理论产量增产率16.95%~7.66%,增产量1 960.36~681.51 kg/hm2,对照增产最多,比晚播10、20 d增产粮食多2.4~2.9倍。为华北地区提高气候资源利用效率和粮食生产提质增效提供参考。 相似文献
107.
108.
介绍了网站信息资源组织的新模式--主题网关的概念与特征;介绍了ASP技术的相关概念;并以中国茶主题网关建设为例,介绍了ASP技术在主题网关建设中的应用. 相似文献
109.
由于我国档案价值鉴定理论和观念的长期滞后,导致人们在认识上存在许多“误区”。一、对“丰富馆藏”的理解片面,效益观念淡漠;二、忽略了对永久、长期档案的价值鉴定;三、档案价值鉴定是否重要是由档案数量的多少来决定的;四、鉴定难,销毁更难。文章详细探讨了产生这些“误区”的原因,以及由此带来的负面效应,并提出了正确的认识和今后努力的方向。 相似文献
110.
2006年《情报科学》文献计量学分析 总被引:1,自引:1,他引:0
赵望娣 《农业图书情报学刊》2007,19(9):147-150
运用文献计量学的方法,对2006年《情报科学》的载文、作者及引文进行了统计分析,研究其载文特点、作者特点及引文特点。 相似文献