Hydrogeochemistry of the Subaé River Impacted by Lead Smelting Activities,Bahia State,Brazil: Geochemical Characterization and Fluxes of Metals

The Subaé river watershed is considered one of the most critical Pb-impacted environments in Brazil and around the world, due to pollutant dispersion during 33 years of lead ore purification in Santo Amaro da Purification. Severe damages have been reported in biota and population, which depends on the Subaé river watershed quality for agriculture, fishing, and shellfish harvesting. This study aims to understand the geochemical characteristics and dynamics of the river close to the former Pb smelter. The river was sampled at eight sites upstream and eight sites downstream the smelter, near the estuary in the Todos os Santos Bay, six times during a year. Immediate analyses were performed by multiprobe. Major ions were measured by chromatography, dissolved metals by ICP-OES in the filtrated samples (0.45 μm), and particulate metals >?0.45 μm by EDX spectrometry. The ions Na⁺ and HCO₃^? are dominated in the river. Most of the samples (47.6%) were classified as sodic, due to oceanic saline intrusion during tide. Despite the high pollution caused by the smelter from 1960 to 1993, still observed in the surrounding soils, dissolved and particulate metals in the river remained low in all sites during the entire year. Only Cu presented some concentration above the threshold of the Brazilian regulations. The discharge of metals by the river into the Todos os Santos Bay appears to be low for Pb and Zn (2.2 and 14.3 kg km^?1 year^?1, respectively), but higher for Cu comparatively to other worldwide bays.     

Assessment of distribution of ventilation and regional lung compliance by electrical impedance tomography in anaesthetized horses undergoing alveolar recruitment manoeuvres

Objective

To examine changes in the distribution of ventilation and regional lung compliances in anaesthetized horses during the alveolar recruitment manoeuvre (ARM).

Growth phenology in <Emphasis Type="Italic">Pinus halepensis</Emphasis> Mill.: apical shoot bud content and shoot elongation

Key message

The chronology of periods of organogenesis and elongation is highlighted in Pinus halepensis.The two first growth units of an annual shoot are preformed inside the bud during the previous year. The following growth units are formed during the spring or summer of the current year.

Context

Analysis of annual shoot length growth phenology is crucial to assess the impact of climate change on tree production. Little is known about the basic growth characteristics and the phenology of pines.

Aims

The present study disentangles the roles of shoot organogenesis vs elongation in the annual growth cycle of the polycyclic Aleppo pine.

Methods

Growth of young Pinus halepensis trees was monitored monthly for 1 year. At each monitoring date, the bud content and meristem dimensions of the main stem shoots apices were analyzed.

Results

The two first growth units of an annual shoot are preformed inside the bud during the previous year. The following growth units are formed during the spring or summer of the current year. The gap between a shoot organogenesis and its elongation may vary from 1 month, for the last growth unit, to half a year, for the first growth units.

Conclusion

Our results underline the importance of taking seasonal environmental conditions from both the previous and the current year into account, in order to study the plasticity of annual shoot growth and its response to climate change and variability.

     

High selfing rate inferred for white fonio [<Emphasis Type="Italic">Digitaria exilis</Emphasis> (Kippist.) Stapf] reproductive system opens up opportunities for breeding programs

In a context of the global major changes, it is mandatory to enlarge the range of crops supporting food security and pay great attention to neglected and underutilized species. However, basic knowledge of the biology of many neglected and underutilized species is still lacking to increase their yields. In this study, the mating system of white fonio [Digitaria exilis (Kippist.) Stapf], a West African minor and promising cereal, is assessed. Progenies arrays from both homozygous and heterozygous mothers were genotyped with microsatellites markers. The rate of genotyping errors in the experiments was assessed and a likelihood framework was used to determine the probability of different mating systems: outcrossing, self-fertilization and apomixis. The results suggested that white fonio has a highly selfing reproductive system with a possible outcrossing rate of 1.7%. Understanding the reproduction system of white fonio opens up opportunities for more effective breeding programs and a wider use of this cereal for food security improvement.     

Identification and characterization of the precursor of chicken matrix metalloprotease 2 (pro-MMP-2) in hen egg

Using zymography and mass spectrometry, we identified for the first time the precursor of chicken matrix metalloprotease 2 (pro-MMP-2) as a complex with TIMP-2 (tissue inhibitor of metalloproteinases) in egg white and yolk. Real-time polymerase chain reaction confirmed that MMP-2 and its inhibitors TIMP-2 and TIMP-3 were expressed all along the oviduct and in the liver of laying hens. We also demonstrated that the processing of pro-MMP-2 into mature MMP-2 by serine proteases does not occur in vivo, although purified pro-MMP-2 undergoes proteolytic maturation by these proteases in vitro. Moreover, the relative pro-MMP-2 activity assessed by gelatin zymography was shown to decrease in egg white during the storage of unfertilized or fertilized eggs. However, the mature form of 62 kDa MMP-2 could not be detected. The fact that MMP-2 is found as a proform in fresh eggs suggests that the activity of this metalloprotease is regulated under specific conditions during embryonic development.     

Water table is a relevant source for water uptake by a Scots pine (Pinus sylvestris L.) stand: Evidences from continuous evapotranspiration and water table monitoring

The objective of this study was to quantify the main terms of the water cycle in a Scots pine stand (Pinus sylvestris L.) growing on a sandy soil and to estimate the contribution of the shallow water table (0.80 m deep in spring) to the forest water use. Continuous monitoring was organized in 2005 to measure climate, throughfall, soil moisture, tree transpiration and water table variations at a half-hourly basis. Leaf area index seasonal dynamic was measured and roots were counted down to the bottom of the soil profile. Forest floor evapotranspiration was modelled with Granier et al. [Granier, A., Bréda, N., Biron, P., Villette, S., 1999. A lumped water balance model to evaluate duration and intensity of drought constraints in forest stands. Ecol. Model. 116, 269–283]. From May to November, pine transpiration never exceeded 1.85 mm d⁻¹ and reached a total of 176.4 mm, which corresponded to 25% of potential evapotranspiration, whereas the understorey evapotranspiration was 130 mm (i.e. 18–20% of the stand water use). The maximum soil water reserve measured over the soil rooted zone was 250 mm, in which 145 mm was extractable water. A 3.5-week period with no rain was observed in June, which induced a regulation of pine transpiration when the soil extractable water reached 0.25 of its maximum value.We applied the water table fluctuation (WTF) method [White, W., 1932. A method for estimating groundwater supplies based on discharge by plants and evaporation from soil. US Geol. Survey Water Supply Paper 659-A. United States Government Printing Office, Washington, DC] to estimate the water table daily loss of water. A relationship was established with potential evapotranspiration and the actual transpiration fluxes of the stand. Yet, it was not possible to extract from the WTF results the part that was effectively contributing to actual transpiration. We applied then the WTF methodology on longer time intervals, with a focus on periods with no rains. From May to November, the contribution of the water table to forest transpiration reached 61%. During the drought period in June, the water table contributed to 98.5% of the water uptake by vegetation, through its contribution to the capillary rise above the water table. The presence of a groundwater table with a floor down to 180–200 cm allowed this stand to rely upon water that otherwise would have drained deeper.     

Selective logging alters allometric relationships of five tropical tree species in seasonal semi-deciduous forests

In selectively logged forests,trees are more likely to expand their diameters(D)at the expense of height(H)growth,resulting in variations in H:D relationships.This study examines how selective logging affects the H:D allometric relationships of five common tree species and whether the effects vary with functional groups(shade-intolerant or shade tolerant)in seasonal semi-deciduous forests.Individuals of five species in a 3000 m^2(0.3 ha)plot were marked and heights and diameters recorded.Most of the species,with one exception,showed greater investment in diameter per increment of height compared to an unlogged forest,possibly because of the greater light available.This study shows the effects of selective logging on species populations as evidenced by increases in H:D ratios.Comparison of forest fragments with different degrees of human impact is important because it allows us to understand the differences in architectural characteristics caused by selective logging.