鲈鲤肌肉脂肪酸组成分析

对鲈鲤肌肉脂肪酸进行测定,结果显示:鲈鲤肌肉中含有7种脂肪酸,饱和脂肪酸有3种,不饱和脂肪酸有4种。3种饱和脂肪酸(SFA)总量为28.22%,4种不饱和脂肪酸(UFA)为71.75%,以不饱和脂肪酸中油酸C18∶1含量最高,为44.28%。鲈鲤鱼体肌肉脂肪酸主要由油酸C18∶1、棕榈酸C16∶0、亚油酸C18∶2和棕榈油酸C16∶14种脂肪酸组成,这4种脂肪酸质量分数总和占脂肪酸总质量分数的93.58%。鲈鲤肌肉脂肪酸种类组成与其它几种淡水鱼类比较,存在明显差异。     

柑桔炭疽菌次生分生孢子形成的研究

 分别用不同温度、湿度、pH、光照对柑桔炭疽菌的不同分离系作单因子和复因子试验,结果表明:次生分生孢子形成的最适温度为23~27℃,最适pH值为6~7,在相对湿度为100%时产孢率最高,需要一定的营养。光对次生分生孢子形成的作用受温度影响,当温度在25℃以上产孢率最高的是黑光与黑暗交替和黑光的处理;温度在25℃以下时产孢率最高的是连续24小时的荧光处理。次生分生孢子形成必需要光。复因子试验的结果表明主效因素为温度,产孢率最高的最优组合为全黑暗-015-查彼培养液-pH8-水-25~29℃及先昼后夜-011-1%蛋白胨液-pH6-水-20~23℃。     

广东鹤山、东莞莲花山小蛾类（Ⅰ）

对广东省鹤山和东莞莲花山自然保护区的小蛾类昆虫进行调查，计有8科35属43种。其中广东新记录种14种。     

酒泉市动物产地检疫现状及对策

随着社会经济迅速发展,酒泉市动物及动物产品流通日益频繁,动物产地检疫极为重要。本文主要论述了酒泉市动物产地检疫的作用、意义、现状、存在的问题及解决对策和措施。     

Three novel single-nucleotide polymorphisms of MBL1 gene in Chinese native cattle and their associations with milk performance traits

Mannan-binding lectin (MBL), a pattern recognizing serum protein, participates in the innate immune system of mammals as an opsonin. In humans, single-nucleotide polymorphisms (SNPs) in MBL2 gene were found to cause various innate immune dysfunctions. In the present study, we discovered three single-nucleotide polymorphisms of the MBL1 gene in Chinese native cattle and analyzed their associations with milk traits. By screening the genetic variation of MBL1 in 1053 individuals of three Chinese native cattle breeds including China Holstein, Luxi Yellow and Bohai Black using created restriction site–polymerase chain reaction (CRS–PCR), PCR–restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing techniques, three new SNPs, g.855G>A, g.2651G>A and g.2686T>C, were found to have allele frequencies of 0–12.65%, 24.07–42.39% and 56.95–73.68%, respectively. While SNP g.855G>A is located within intron ?, the other two SNPs reside in the exon II region with one mutation being non-synonymous (GTT (Val) > ATT (Ile)) and the other synonymous (GCT (Ala) > GCC (Ala)). Among the 596 Chinese Holstein cattle with at least 3 lactation Dairy Herd Improvement (DHI) records, eight different haplotypes and 19 genotype combinations were detected. Statistical analyses revealed no correlation between either g.855G>A or g.2686T>C and somatic cell score (SCS), however significant association was found between g.2651G>A and SCS, suggesting a possible role of this SNP in the host response against mastitis. Our data also suggested that the combined genotypes of GGC/AAC with the lowest SCS, AAT/AAT with the highest protein content and AGC/AGC with the highest 305-d milk yield were favorable combinations for mastitis resistance and milk production traits. Therefore, GGC/AAC, AAT/AAT and AGC/AGC can be used as possible candidates for marker-assisted selection in the dairy cattle breeding program.     

基层兽医机构防控非洲猪瘟效能评估与分析

为加强基层动物防疫体系建设,客观评估基层兽医机构防控非洲猪瘟能力和非洲猪瘟防控政策执行情况,组织开展了10个养猪大省的20个县基层兽医机构防控非洲猪瘟效能评估。结果显示：当前基层兽医机构具备防控非洲猪瘟的基本能力,但已不适应新时期动物疫病防控需要,部分关键能力存在不足;发生疫情县的防控能力普遍低于未发生疫情县,说明“人财物”资源配置好的地区防控非洲猪瘟等重大动物疫病的能力较强;政策落实情况与兽医机构效能总体上呈正相关性,技术性政策落实情况明显优于资源性政策落实情况。此次效能评估工作引起了地方政府重视,切实发挥了评估“指挥棒”作用,为各地加强基层动物防疫体系建设发挥了示范带动作用。     

超高效液相色谱质谱联用检测苹果和土壤中丙撑硫脲的残留分析方法

建立了丙撑硫脲在苹果和土壤中的残留分析方法。样品经提取乙腈提取、PSA净化后质谱检测器检测。结果表明:添加质量分数为0.01~0.5mg/kg时,丙撑硫脲在苹果和土壤中平均添加回收率分别为76.5%~98.3%和84.9%~99.0%,相对标准偏差分别为3.1%~4.9%和3.2%~9.0%。丙撑硫脲在苹果中的最低检出质量分数为0.01mg/kg。该方法快速简便,准确可靠。     

香蕉束顶病毒(BBTV)侵染对寄主内源激素的影响

 本试验用ELISA方法测定了香蕉感染束顶病毒(BBTV)后植株体内的3种内源激素,赤霉素(GA₃)、玉米素类(iPAs)和脱落酸(ABA)的变化。结果表明,感株的GA₃水平在侵染过程中虽有微弱增加,但含量和增长速度显著低于健株对照;iPAs在接种BBTV第14天后明显下降,并维持较低水平;ABA在BBTV侵染后被大量诱导增加并不断积累,在接种后第35天测定含量最高,为对照的3.34倍。试验还同时检测了BBTV在侵染过程中的运转。用间接ELISA测定的接种叶和顶叶的BBTV含量显示:接种21天后BBTV在接种叶和顶叶中还大量增殖,呈系统性分布。但寄主的症状在接种35天后才逐渐在顶叶表现。以上结果表明:香蕉束顶病的症状表现似乎主要与病株中的内源激素的失调有关,而与BBTV在体内的运转并不直接相关     

山东省棕榈蓟马田间种群对7种杀虫剂的抗药性

采用叶管药膜法测定了山东省4个蔬菜产区棕榈蓟马对7种杀虫剂的抗药性水平。结果表明,供试药剂毒力由大到小依次为多杀霉素甲氨基阿维菌素苯甲酸盐吡虫啉噻虫嗪吡蚜酮高效氯氟氰菊酯辛硫磷;济南、聊城和泰安地区的棕榈蓟马种群对7种药剂基本处于敏感状态,寿光地区的棕榈蓟马对多杀霉素、甲氨基阿维菌素苯甲酸盐、吡虫啉和吡蚜酮产生了6.99~10.83倍的低水平抗性。