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41.
42.
Itaru Yamaguchi Kensuke Myojo Hiroko Sanada Eri Sudo Sayaka Ootsuka Hiroshi Okumura Atsuko Takami Tomomi Yoneshige Yui Suzuki Minami Imaizumi Chie Takada Naoya Kimoto Koji Saeki Katsumi Takaba 《Journal of toxicologic pathology》2013,26(3):301-307
We histopathologically and immunohistochemically investigated a case of malignant
lymphoma that spontaneously developed in a male common marmoset at two years of age.
Beginning at two years four months of age, the animal had an enlargement of the
submandibular and inguinal lymph nodes, small subcutaneous nodules near the right breast
and an approximately fivefold increase in peripheral lymphocyte count compared with the
previous examination value. The postmortem findings at two years eight months of age
showed lymphadenopathy with enlargement of the thymus and spleen. Small- to
intermediate-sized neoplastic lymphocytes had diffusely proliferated in the enlarged
nodes. The neoplastic cells were pleomorphic and had irregularly shaped nuclei. The
nuclear chromatin staining revealed hyperchromatism in the small-sized cells, and the
intermediate-sized cells exhibited vesicular staining. An immunohistochemical examination
indicated that the neoplastic lymphocytes were positive for CD3 and negative for CD20,
thus suggesting that they had originated from T cells. In addition, the proliferation of
high endothelial venules and reactive epithelioid histiocytes was observed. Scattered
tingible body-laden macrophages were infrequently detected. Neoplastic lymphocytes were
also observed in the thymus, spleen, heart, lungs, liver, kidneys, adrenal glands and
femoral and sternal bone marrow. This malignant lymphoma in a young male common marmoset
was considered to fit the category of “peripheral T-cell lymphoma, not otherwise specified
(PTCL-NOS)” according to the new WHO system of classification. 相似文献
43.
Toshihisa SUGINO Yuko KAWAKITA Rika FUKUMORI Yoshihisa HASEGAWA Masayasu KOJIMA Kenji KANGAWA Taketo OBITSU Kohzo TANIGUCHI 《Animal Science Journal》2010,81(2):199-204
Two experiments were conducted to elucidate the effects of post‐ruminal administration of starch and casein (Exp. 1), plasma amino acids concentrations (Exp. 2), and plasma glucose and insulin concentrations (Exp. 2) on plasma ghrelin concentrations in sheep. In Exp. 1, plasma ghrelin concentrations were determined by four infusion treatments (water, cornstarch, casein and cornstarch plus casein) in four wethers. Abomasal infusion of casein increased plasma α‐amino N (AAN) concentrations. Infusion of starch or casein alone did not affect plasma ghrelin concentrations, but starch plus casein infusion increased plasma levels of ghrelin, glucose and AAN. In Exp 2, we investigated the effects of saline or amino acids on ghrelin secretion in four wethers. Two hours after the initiation of saline or amino acid infusion into the jugular vein, glucose was also continuously infused to investigate the effects of blood glucose and insulin by hyper‐glycemic clump on plasma ghrelin concentrations. Infusion of amino acids alone raised plasma levels of ghrelin, but the higher plasma glucose and insulin concentrations had no effect on plasma ghrelin concentrations. These results suggest that high plasma levels of amino acids can stimulate ghrelin secretion, but glucose and insulin do not affect ghrelin secretion in sheep. 相似文献
44.
Fujimoto Y Nakatani N Kubo T Semi Y Yoshida N Nakajima H Iseri T Azuma YT Takeuchi T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(1):27-34
Macrophages are essential for controlling the majority of infections, and are mediators of natural immunity. During infection, lipopolysaccharide (LPS) stimulates macrophages to produce pro-inflammatory cytokines. Adenosine and ATP released into the extracellular space by immunological stimuli have been shown to regulate various immune functions. More recently, it has been shown adenosine and ATP have a critical role on the physiological negative feedback mechanism for limitation and termination of tissue-specific and systemic inflammatory responses. It was useful and meaningful to gain information about interaction between LPS, which generates the inflammation, and adenosine and ATP, which terminate the inflammation. We evaluate effects of adenosine and ATP on the production of cytokines related to inflammation in canine macrophage cell line DH82 cells. Adenosine and ATP respectively increased the production of IL-10 without affecting the production of IL-6, TNF-α and IL-12 in DH82 cells. In addition, adenosine and ATP prevented the production of LPS-induced IL-6, TNF-α and IL-12 in DH82 cells. In contrast, adenosine and ATP potentiated LPS-induced IL-10 production in DH82 cells. Moreover, adenosine, but not ATP inhibited LPS-induced expression of TLR4 in DH82 cells. These results suggest that conditions related to increased adenosine and/or ATP may play an important role in the inflammatory reactions. 相似文献
45.
Makimi Wakai Shusuke Hayashi Yuko Chiba Satoshi Koike Kyo Nagashima Yasuo Kobayashi 《Animal Science Journal》2021,92(1):e13598
The growth and morphology of rumen methanogenic archaea (15 strains of 10 species in 5 genera, including 7 strains newly isolated in the present study) and bacteria (14 species in 12 genera) were investigated using unsupplemented in vitro pure cultures and cultures supplemented with cashew nut shell liquid (CNSL) and its phenolic compound components, anti-methanogenic agents for ruminant animals. Growth of most of the methanogens tested was inhibited by CNSL and alkylphenols at different concentrations ranging from 1.56 to 12.5 μg/ml. Of the alkylphenols tested, anacardic acid exhibited the most potent growth inhibition. Three gram-negative bacterial species involved in propionate production were resistant to CNSL and alkylphenols (>50 μg/ml). All the methanogens and bacteria that were sensitive to CNSL and alkylphenols exhibited altered morphology; disruption of the cell surface was notable, possibly due to surfactant activity of the tested materials. Cells division was inhibited in some organisms, with cell elongation and unclear septum formation observed. These results indicate that CNSL and alkylphenols, particularly anacardic acid, inhibit both rumen bacteria and methanogens in a selective manner, which could help mitigate rumen methane generation. 相似文献
46.
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49.
Uchida Y Ike K Katayama N Oguni Y Imai S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(5):539-542
Interleukin 10 (IL-10) genes of Djungarian, Chinese, and Syrian hamsters were cloned. The clones of IL-10 consisted of 537 bp nucleotides and 178 amino acids in full length, and the nucleotide and amino acid sequences exhibited a high degree of homology with those of the mouse and human. Since the number and position of signal sequences, N-glycosylations and cysteine sites in the IL-10 amino acid sequences of the hamsters were the same as those of the mouse, we suggest that the IL-10 molecular structures of the hamster are closer to that of the mouse than human. 相似文献
50.
Katsuhiko Yoshizawa Yuichi Kinoshita Yuko Emoto Ayako Kimura Norihisa Uehara Takashi Yuri Nobuaki Shikata Airo Tsubura 《Journal of toxicologic pathology》2013,26(2):141-148
N-Methyl-N-nitrosourea (MNU)-induced renal tumors in rats and Wilms tumors in humans were compared. Renal mesenchymal tumors (RMTs) and nephroblastomas (blastemal and epithelial components) in female Lewis rats treated with a single intraperitoneal injection of 50 mg/kg MNU at birth and Wilms tumors (blastemal, epithelial and mesenchymal components) in humans were analyzed for the expression of pancytokeratin (CK), vimentin, p63, α-smooth muscle actin (SMA), desmin, S-100, CD57, CD117/c-kit, Wilms tumor 1 protein (WT1) and β-catenin. The mesenchymal components of rat RMTs and human Wilms tumors expressed vimentin, SMA and β-catenin. The blastemal components of rat nephroblastomas and human Wilms tumors expressed vimentin, CD117/c-kit and β-catenin. The epithelial components of rat nephroblastomas and human Wilms tumors expressed vimentin and β-catenin. WT1 was expressed in different cellular components of rat tumors as compared with human Wilms tumors; the expression was seen in mesenchymal tumors and blastemal components of nephroblastomas in rats and epithelial components in human Wilms tumors. CK, p63 and CD57 were not expressed in rat RMTs or nephroblastomas, while CK and WT1 were expressed in epithelial components and CD57 was expressed in blastemal and epithelial components of human Wilms tumors. Rat and human tumors were universally negative for the expression of desmin and S-100. The immunohistochemical characteristics of rat renal tumors and human Wilms tumors may provide valuable information on the differences in renal oncogenesis and biology between the two species. 相似文献