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81.
82.
通过高效凝胶过滤层析和反相高效液相色谱先后对大米渣蛋白酶解后的多肽溶液进行分离纯化,分别得到组分2和组分RF9;再经反相高效液相色谱-质谱联用对组分RF9进行分析鉴定得到抗氧化肽氨基酸序列L-Q-P-Y(Leu-Gln-Pro-Tyr),分子质量为520.277u。合成的抗氧化肽片段L-Q-P-Y质量浓度为1 mg/mL时,对DPPH自由基清除率达到85.84%。 相似文献
83.
腐乳作为我国传统发酵豆制食品,经过不断的工艺改进,已经逐步形成一定生产规模。但由于生产技术限制,我国腐乳生产还存在着生产发酵时间较长、盐含量较高、安全性有待提升等品质问题,这些问题大大限制了该行业的发展。为提高腐乳生产质量,从生产流程入手,针对生产流程中包括制胚、微生物接种、食盐添加、发酵条件等方面进行分析,对可能影响腐乳最终品质的因素进行概述,通过对以上提出问题逐个击破,提出解决方案,改进制作工艺,最终提升腐乳品质。 相似文献
84.
Jie Li Yuanliang Shi Jiafa Luo David Houlbrooke Stewart Ledgard Anwar Ghani Stuart Lindsey 《Journal of Soils and Sediments》2014,14(8):1341-1349
Purpose
With land application of farm effluents from cows during housing or milking as an accepted practice, there are increasing concerns over its effect on nitrogen (N) loss through ammonia (NH3) volatilization. Understanding the relative extent and seasonal variation of NH3 volatilization from dairy effluent is important for the development of management practices for reducing NH3 losses. The objectives of this study were to determine potential NH3 losses from application of different types of dairy effluent (including both liquid farm dairy effluent (FDE) and semi-solid dairy farm manure) to a pasture soil during several contrasting seasons and to evaluate the potential of the urease inhibitor (UI)—N-(n-butyl) thiophosphoric triamide (NBTPT, commercially named Agrotain®) to reduce gaseous NH3 losses.Material and methods
Field plot trials were conducted in New Zealand on an established grazed pasture consisting of a mixed perennial ryegrass (Lolium perenne L.)/white clover (Trifolium repens L.) sward. An enclosure method, with continuous air flow, was used to compare the effects of treatments on potential NH3 volatilization losses from plots on a free-draining volcanic parent material soil which received either 0 (control) or 100 kg N ha?1 as FDE or manure (about 2 and 15 % of dry matter (DM) contents in FDE or manure, respectively) with or without NBTPT (0.25 g NBTPT kg?1 effluent N). The experiment was conducted in the spring of 2012 and summer and autumn of 2013.Results and discussion
Results showed that application of manure and FDE, both in fresh and stored forms, potentially led to NH3 volatilization, ranging from 0.6 to 19 % of applied N. Difference in NH3 losses depended on the season and effluent type. Higher NH3 volatilization was observed from both fresh and stored manure, compared to fresh and stored FDE. The difference was mainly due to solid contents. The losses of NH3 were closely related to NH4 +-N content in the two types of manure. However, there was no relationship between NH3 losses and NH4 +-N content in either type of FDE. There was no consistent seasonal pattern, although lower NH3 losses from fresh FDE and stored FDE applied in spring compared to summer were observed. Potential NH3 losses from application of fresh FDE or manure were significantly (P?<?0.05) reduced by 27 to 58 % when NBTPT was added, but the UI did not significantly reduce potential NH3 volatilization from stored FDE or manure.Conclusions
This study demonstrated that NH3 losses from application of FDE were lower than from manure and that UIs can be effective in mitigating NH3 emissions from land application of fresh FDE and manure. Additionally, reducing the application of FDE in summer can also potentially reduce NH3 volatilization from pasture soil. 相似文献85.