对虾皮下及造血组织坏死杆状病毒的精细结构、核酸、多肽及血清学研究

对纯化的对虾皮下及造血组织坏死杆状病毒（ＨＨＮＢＶ）进行了超微结构、紫外吸收、核酸类型及多肽ＳＤＳ－ＰＡＧＥ的研究。同时用单克隆抗体对ＨＨＮＢＶ的两分离株和ＭＢＶ进行了血清学比较研究。用ＴＭＶ作为内标定物测得病毒粒子大小为１５０ｎｍ×４５０ｎｍ，其衣壳为两个帽状物端夹１３圈螺旋对称的园柱体结构。病毒在２６０ｎｍ处吸光系数为０．１１７ｍｇ／ｍＬ／ＯＤ２６０。病毒核酸为双链ＤＮＡ，分子量大于３５×１０６ｄ。病毒囊膜有１２种主要多肽。病毒衣壳有两种主要多肽。从寿光（ＨＨＮＢＶ—９３７）和青岛（ＨＨＮＢＶ—９３８）分离到的病毒种在ＳＤＳ—ＰＡＧＥ和抗ＨＨＮＢＶ—９３７单抗ＥＬＩＳＡ反应上没有显著差异。ＨＨＮＢＶ与斑节对虾杆状病毒（ＭＢＶ）在抗原决定簇上存在差别。     

饲喂中草药和微量元素硒提高蛋的营养价值的研究

本项研究系应用中草药和微量元素硒配伍的特制预混料，对蛋鸡进行了３次饲养试验。试验结果表明，蛋鸡在饲喂了这种特制预混料后所产的蛋平均含硒量可稳定地由对照组的每枚４３μｇ，提高到１２０～２７５μｇ，成为一种富硒蛋。这种蛋经实验动物安全试验和人医临床试验，均无毒副反应。受试对象按规定量连续服用３个月后表现在血清硒、发硒的含量和血清中谷胱甘肽过氧化物酶（ＧＳＨ—Ｐｘ）的活性均比服用前明显提高，差异极显著（Ｐ＜０．０１）。这些指标的升高表明富硒蛋可以方便、安全地补充人体生理需要量的有机硒．作者认为这种富硒蛋可以提高人体的免疫功能和抗衰老以及预防心血管疾病等作用，将为人们提供一种多功能性的天然绿色保健食品。     

对阜新地区农机维修管理工作的探讨

随着农业机械化发展进程的不断加快和农业机械数量的快速增加,农机社会化服务领域也随之不断拓宽.农机维修服务作为保障农机作业质量和安全的关键环节,对其服务质量的提高、技术水平的升级提出更高要求.为强化农机维修工作的地位和作用,就新时期阜新地区农机维修工作进行探讨.     

Symbiotic N<Subscript>2</Subscript> fixation and nitrate utilisation in irrigated lucerne (<Emphasis Type="Italic">Medicago sativa</Emphasis>) systems

Symbiotic N₂ fixation by lucerne (Medicago sativa) has capacity to provide significant inputs of N to agro-ecosystems, and the species has also been shown to scavenge soil mineral N and thus act as a sink for excess reactive N. The balance between these two N cycle processes was investigated in an extensive irrigated lucerne growing region where nitrate contamination of groundwater has been reported. We sampled 18 permanent pure lucerne stands under irrigation for standing dry matter, total shoot N, and N₂ fixation using ¹⁵N natural abundance along with activity of the inducible enzyme nitrate reductase as indicators of use of soil NO₃⁻ by lucerne. On average 65% of lucerne N was obtained from symbiotic N₂ fixation. Converting standing dry matter estimates to annual N₂ fixation amounts we calculated average N₂ fixation of 311 kg N/ha, including N in roots and nodules. Uptake of N from soil by lucerne was calculated to be 181 kg N/ha/year. We were not able to identify the source of this soil mineral N, although nitrate reductase activity of lucerne was higher than that of non-N₂ fixing species examined.     

健康苗识别标准数据库的构建

为实现应用计算机图像处理技术进行健康苗识别,必须建立健康苗的标准数据库.为此,通过对健康苗的定性描述,建立了健康苗的判别准则、判别模型,构建了健康苗识别标准数据库.该数据库实现了健康苗的识别,提高工厂化集中育苗的自动化、智能化程度.     

<Emphasis Type="Italic">Pinus halepensis</Emphasis> somatic embryogenesis is affected by the physical and chemical conditions at the initial stages of the process

Pinus halepensis has been described as a drought-tolerant species with high plasticity to growth in different environments. Its eco-physiological characteristics could facilitate the use of this species in large afforestations in the future scenery of climate change. Somatic embryogenesis is a biotechnological tool with potential for large-scale clonal propagation. In order to establish an improved regeneration protocol for Pinus halepensis, the effects of different temperatures (18, 23, and 28 °C) and water availability conditions (2, 3, and 4 g L^?1 Gelrite^®), during initiation of embryonal masses on the rate of initiation, proliferation, maturation, and the number of embryos developed, were evaluated. It was found that environmental conditions during the initiation stage of Pinus halepensis somatic embryogenesis influence the success of initiation and proliferation. In contrast, there was no effect of these conditions on the maturation rates and the number of somatic embryos. Somatic embryos were obtained in all treatments tested, indicating that plants can be produced from extreme conditions of induction, such as high temperatures (28 °C) and low water availability conditions (4 g L^?1).     

云南省彝良县农业产业发展的战略思考

随着经济一体化趋势的加剧,农业发展的目标、条件与环境也发生了根本变化,农业发展的主要制约因素已从过去的资源约束为主转向以需求和资源双重约束为主。通过对彝良县农业产业发展情况与制约因素分析,提出彝良县农业产业发展的思路、目标、重点及保障措施。     

生姜群体光台特性的研究

利用田间同化箱对生姜的群体光合特性进行了研究。结果表明，生姜群体光合速率（ＣＰｎ）有明显的季节变化和日变化，随着季节的进展ＣＰｎ呈二次曲线型。ＣＰｎ的日变化呈单峰曲线，峰值出现在中午１２时左右。ＣＰｎ的峰值与旺盛生长季节相吻合。在本试验范围内单位土地面积的ＣＰｎ随ＬＡＩ的增加而增加，若以叶面积计算ＣＰｎ则随叶面积指数（ＬＡＩ）的增加而降低。在田间试验条件下ＣＰｎ随光强和ＣＯ２浓度的增加而增加，在自然光下未测到生姜ＣＰｎ的光饱和点。     

农村中小学现代远程教育资源利用的问题与策略

随着农村中小学现代远程教育工程的逐步实施和深入,农村中小学在利用农村远程教育资源（以下简称农远资源）的过程中也暴露出越来越多的问题。针对农远资源在农村中小学教育中利用率低的问题,在做了相关调查的基础上,分析了农村中小学农远资源利用中存在的弊端,为提高农远资源的利用率提出了相关的解决策略。     

古田山国家自然保护区常绿阔叶林植物物种多样性研究

分析了古田山自然保护区常绿阔叶林植物区系和物种多样性.结果表明:古田山自然保护区常绿阔叶林植物种类十分丰富,计有维管束植物64科114属198种(含变种),其中蕨类植物7科8属19种,裸子植物2科2属2种,被子植物55科104属177种.种子植物区系成分复杂,具有较高的热带成分比例.不同群落类型其物种多样性不同,4种常绿阔叶林群落类型中以栲树林物种多样性最高,甜槠-木荷林物种多样性最低.在群落垂直结构中,除钩栗林外,灌木层→乔木层→草本层物种多样性依次降低.乔木层与灌木层的物种多样性指数差异不显著,而乔木层、灌木层的物种多样性指数均显著大于草本层.