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991.
Yu Lu Ma 《Soil Science and Plant Nutrition》2013,59(4):628-641
Two pot experiments were conducted to evaluate biochar derived from dead dairy cattle as a mineral fertilizer, especially phosphorus (P) fertilizer, and to clarify the effect of particle size of biochar on plant growth (Zea mays L.) and P uptake. To produce the biochar, body parts of dead cattle were placed in a charring chamber and allowed to char at 450°C for 4 h. The biochar was of high pH and rich in major plant nutrients, especially P. Application of fine biochar (< 1 mm) increased P uptake by the corn plants grown in soil of low available P status. As a result, plant growth was improved following biochar application and dry matter production was also increased. The effect of the biochar application on the P uptake and plant growth was promoted by the application of mineral nitrogen (N) fertilizer. Soil analysis after harvest indicated that the biochar application increased soil pH, available P and exchangeable calcium (Ca) and magnesium (Mg) compared with the soil before seeding, while soil available N and exchangeable potassium (K) were considerably decreased. The decrease in the soil available N was incomprehensible, because the result of the mass balance given by the difference between input as the applied N from the biochar and fertilizer N and output as the N uptake by the plants was positive. We observed a similar result in the mass balance of K to the case of N. The medium (2–4 mm) and coarse (> 4 mm) grade biochar did not significantly affect plant growth, because P uptake was not, or was only slightly, increased by the application of these biochars. Dissolution of P from the coarser biochars was probably slower than that of the fine biochar. The lower dissolution of P from the medium and coarse biochars was supported by the lower P absorption efficiency of these biochars compared with that of the fine biochar and superphosphate. The effect of fine biochar on plant growth and P uptake was similar to that of superphosphate. We can therefore conclude that fine biochar derived from cattle carcasses is an effective source of P fertilizer and amendment for soil acidity. The N and K contents in the biochar, although relatively high, cannot be relied upon as a mineral fertilizer. Further studies are needed to assess whether the N and K contents of the biochar indicate it can be regarded as a useful fertilizer. 相似文献
992.
本研究提供了一种新的整合多拷贝外源基因表达单元到枯草芽孢杆菌染色体同一位点的方法。以β-淀粉酶表达单元作为应用实例,本方法包括以下步骤:首先,构建含有一个拷贝β-淀粉酶表达单元的整合质粒pMLK83-CTBA,通过同源双交换获得单拷贝β-淀粉酶表达单元整合到枯草芽孢杆菌1A751染色体α-淀粉酶基因位点的菌株1A751[CTBA]Neo+;然后,利用抗性基因替换质粒pVK71,将新霉素抗性基因替换为状观霉素抗性基因,得到1A751[CTBA]Neo-Spe+;最后,整合质粒pMLK83-CTBA再以同源单交换方式整合到1A751[CTBA]Neo-Spe+染色体,通过新霉素抗性和状观霉素抗性筛选出两个拷贝β-淀粉酶基因整合的重组菌1A751[CTBA2]Neo+Spe+。结果显示,利用此方法增加β-淀粉酶基因的拷贝数,能够显著和稳定地提高β-淀粉酶的表达量。 相似文献
993.
994.
Influence of management practices on soil organic matter changes in the Northern China plain and Northeastern China 总被引:4,自引:0,他引:4
Yan Xu Fengrong Zhang Xiying Hao Jingkuan Wang Ru Wang Xiangbin Kong 《Soil & Tillage Research》2006,86(2):230-236
Soil organic matter (SOM) is strongly related to soil type and management practices. Changes in government policy have brought drastic changes in farm management practices in the last two decades in rural China. This study investigates changes in SOM in two different soils: Ustepts and Udolls. Ustepts, in the North China Plain where the climate is warm and sub-humid, developed from an alluvial flood plain with organic matter <10 g kg−1. Udolls, in Northeastern China where the climate is cool and sub-humid, developed from loess-like materials with organic matter >20 g kg−1. Two locations for Ustepts and three locations for Udolls were used to collect 567 soil samples in 1980–1982 and again in 2000 for SOM analysis. Soil organic matter increased for Ustepts and decreased for Udolls soils over the sampling period, resulting from differences in fertilizer rates and crop residue input to soil. Higher fertilizer input and crop intensity and initially very low SOM content in Ustepts all contributed to greater OM input than oxidation release. In contrast, lower fertilizer input and crop intensity, and initially high SOM content in the Udolls, led to lower OM input than oxidation release. Increasing SOM content through higher mineral fertilizer input is a valuable option for sustainable agriculture production in areas where SOM is low and there is a shortage or potential shortage of food supply. 相似文献
995.
Yu. N. Vodyanitskii 《Eurasian Soil Science》2006,39(10):1074-1083
The analysis of published data shows that the sequential extraction of heavy metals from soils according to the schemes suggested by Tessier and by the European Community Bureau of Reference (BCR) does not ensure the selectivity of the extraction of heavy metal compounds from certain carriers and can result in the artificial redistribution of heavy metals by fractions. A new approach is based on the assessment of the kinetics of metal extraction from certain carriers. A wide range of heavy metal compounds in soils have been identified using the method of synchrotron X-ray radiation. It is argued that the schemes of Tessier and the BCR are applicable for the determination of the contents of Pb humates, Pb and Zn carbonates, and heavy metals bound with hydroxides of Fe and Al. However, these methods are not suitable for the determination of phosphates and silicates of heavy metals. 相似文献
996.
Rain-fed agriculture is widespread in Inner Mongolia, northern China, where wind erosion of farmland is very common because of sandy soil and dry, windy weather. However, very little is known about the effects of wind erosion on soil physical and chemical properties in this region. A field experiment was conducted in sandy farmland, where erosional and depositional gradients were established to evaluate the effects of wind erosion and leeward sand accumulation on soil texture, nutrient content, soil water, and soil temperature. The research showed that long term wind erosion could result in significant soil coarseness, infertility and dryness. Severe erosion reduced clay by 59.6%, organic C by 71.2%, total N by 67.4%, total P by 31.4%, available N by 64.5%, available P by 38.8%, and average soil water content by 51.8%, compared with non-eroded farmland in the study region. The sand fraction (particles > 0.05 mm), pH and ground-surface temperature increased by 6.2%, 3.7%, and 2.2 °C, respectively. Accumulated sand also caused a decrease in nutrients and soil water content. Under severe sand accumulation, clay was reduced by 2.0%, organic C by 19.3%, total N by 21.7%, total P by 13.7%, available N by 52.5%, and average soil water content by 26.6%. The sand fraction, pH, available P, and ground-surface temperature increased by 0.2%, 0.9%, 5.8% and 2.8 °C, respectively. 相似文献
997.
998.
基于粗集权重的改进可拓评价方法在灌区干旱评价中的应用 总被引:9,自引:2,他引:9
在分析了灌区干旱评价特点和目前评价方法不足的基础上,引入了基于非对称贴近度和粗集理论的改进可拓评价方法,有效避免了灌区干旱评价指标界限的纲性量化导致的遗漏问题和单项指标评价结果的矛盾性、不确定性和不相容性。与传统可拓评价方法相比,在指标权重的确定中采用经验因子协调主客观权重,克服了以往决策者过分依赖专家经验知识确定的不足。在等级评价中采用非对称贴近度原则,代替最大隶属度,有效解决最大隶属度原则的失效问题。计算实例表明,采用基于粗集权重的改进可拓评价方法进行灌区干旱评价是切实可行的。 相似文献
999.
反相HPLC法快速测定鸭蛋蛋黄粉中胆固醇含量 总被引:2,自引:0,他引:2
通过RP-HPLC方法,比较了不同的流动相、流速、检测波长和温度对鸭蛋蛋黄粉中胆固醇分离效果的影响。确定试验最佳条件为:流动相:乙睛:甲醇:异丙醇=50∶80∶10(体积比),柱温45℃,进样量20 μL,流速1.3 mL/min,检测波长205 nm。在此条件下测得的天然红心鸭蛋蛋黄粉和普通鸭蛋蛋黄粉中胆固醇的含量分别为24.84 mg/g和25.75 mg/g。 相似文献
1000.
根据番茄生物学特性、发育阶段有效积温恒定的原理和多年的栽培经验,对温室长季节栽培番茄的发育阶段进行划分,其生长发育阶段包括播种期、幼苗期、开花座果期、果实膨大期、果实采收初期、果实采收盛期和果实采收末期.将不同播期各生育阶段的生长度日的平均值确定为建模过程中的参数Ai自幼苗期至果实采收末期分别为710.5、110.5、152.3、302.9、245.6、2156.7、200.5度日.确定了发育阶段有效积温参数后,建立了温室番茄长季节栽培的发育动态模拟模型,系统的预测番茄发育阶段.模型检验结果表明,温室番茄发育动态模拟模型具有较高的精确性、机理性和实用性. 相似文献