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31.
Tsujimura Y Watarai S Uemura A Ohnishi Y Kodama H 《Research in veterinary science》2005,78(3):245-247
The effect of antibody against ganglioside antigen on Trypanosoma brucei parasites was examined in vitro and in vivo using anti-ganglioside GM1 (AGM-1) monoclonal antibody. The antibody showed complement-dependent cytotoxicity against T. brucei with mouse complement. Furthermore, mice given AGM-1 were challenged intraperitoneally with T. brucei. Although all non-treated control mice died within six days after infection, all of AGM-1-injected mice had survived by six days post-infection. These data suggest that antibody against ganglioside antigen on T. brucei has potential in protection against T. brucei infection. 相似文献
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Wei Wei Lan Yoshie Nishiwaki Seishi Akino Norio Kondo 《Journal of General Plant Pathology》2013,79(3):182-193
In August 2010, bacterial soft rot was found on root chicory (Cichorium intybus var. sativum) in Hokkaido, Japan. Severely infected plants in fields were discolored, had wilted foliage, and black necrosis of petioles near the crown. Wilted leaves subsequently collapsed and died, forming a dry, brown or black rosette. The root and crown became partially or wholly soft-rotted. Slimy masses on infected areas of roots, turned dark brown or black. Gram-negative, rod-shaped, peritrichously flagellated, facultatively anaerobic bacteria were exclusively isolated from rotted roots, and typical symptoms were reproduced after inoculation with the strains. The bacteria were identified as Dickeya dianthicola, Pectobacterium carotovorum subsp. carotovorum, and Pectobacterium carotovorum subsp. odoriferum based on further bacteriological characterization and the sequence analysis of the malate dehydrogenase gene and 16S rRNA gene. These bacteria should be included with the previously reported Dickeya (=Erwinia) chrysanthemi in Saitama Prefecture, Japan, as causal pathogens of bacterial wilt of chicory. 相似文献
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Yurie KAWASAKI Yuka AOKI Fumie MAGATA Akio MIYAMOTO Chiho KAWASHIMA Takuo HOJO Kiyoshi OKUDA Koumei SHIRASUNA Takashi SHIMIZU 《The Journal of reproduction and development》2014,60(3):173-178
The present study aimed to assess the effect of polymorphisms in the tumor necrosis factor α (TNF-α) promoter (A/A, A/G and
G/G) and exons (T/T, T/C and C/C) on immune function and reproductive performance in dairy cows. The occurrence of the first
postpartum ovulation within 3 weeks in the cows with the TNF-α promoter A/G and G/G genotypes was higher than in the A/A
group. Among the different TNF-α exon genotypes, the occurrence of early first postpartum ovulation was higher in the T/C and
C/C genotype groups than in the T/T group. Single nucleotide polymorphisms (SNPs) in the TNF-α gene did not affect the rate
of artificial insemination (AI) or duration from parturition to next conception (days open). The apoptosis rate of
polymorphonuclear leukocytes (PMNs) did not differ among the TNF-α promoter genotypes, but the PMN transmigration rate was
significantly higher for the A/A and A/G genotypes than for the G/G genotype. Interleukin 8 (IL-8) mRNA expression in PMNs
and peripheral blood mononuclear cells (PBMCs) before culture was significantly higher for the A/A genotype compared with the
G/G genotype. There were no significant differences between the genotypes in the mRNA expression of TNF-α, IL-6, IL-1β, and
toll-like receptor 4 (TLR4) in PMNs and PBMCs before and 4 h after culture. IL-8 and IL-1β production by PBMCs cultured for 4
h was significantly higher for the animals with the A/A genotype than for those with the G/G genotype. On the other hand, no
significant difference was observed in IL-8 and IL-1β production by PMNs among different TNF-α genotypes. Taken together,
these results suggest that SNP in the TNF-α gene affects immune function and reproductive performance in dairy cows. 相似文献
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Fukutaro Karu Toru Kobari Taiga Honma Takeru Kanayama Koji Suzuki Naoki Yoshie Gen Kume 《Fisheries Oceanography》2020,29(5):442-456
Many migratory fishes reproduce and recruit around the Kuroshio, and their survival of early life stages is supported by mesozooplankton. Mesozooplankton standing stocks and productivity equivalent to those on the continental shelf have been found in the Kuroshio; however, there is limited information on trophic sources and linkages to support the mesozooplankton community. Here, we evaluate mesozooplankton feeding on protists and their trophodynamics importance by removal bottle experiments in the Kuroshio of the East China (ECS‐Kuroshio). Pico‐ and nano‐autotrophs dominated chlorophyll a concentrations throughout the study sites across the continental shelf, within the Kuroshio path and in adjacent waters. Calanoid and poecilostomatoid copepods comprised more than 85% of mesozooplankton biomass. Significant mesozooplankton ingestion rates were found for nano‐autotrophs based on size‐fractionated chlorophyll; for haptophytes, chrysophytes, chlorophytes, and diatoms from pigment‐based phytoplankton analysis; and for naked ciliates in the microzooplankton. Based on the estimates of individual carbon budgets, nano‐autotrophs and naked ciliates ingested by mesozooplankton composed 39% of their food requirements, suggesting other available prey like nano‐heterotrophs. These results imply that mesozooplankton ingestion in the ECS‐Kuroshio has great impacts on protozoan and phytoplankton communities and their major trophic pathways are from nano‐sized auto‐ and heterotrophs and ciliates to copepods. 相似文献
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Differences in the biochemical compositions of two dietary jellyfish species and their effects on the growth and survival of Ibacus novemdentatus phyllosomas 下载免费PDF全文
Phyllosomas (planktonic larvae) of slipper lobsters cling onto and feed on jellyfish under both natural and laboratory conditions. Phyllosomas of Ibacus novemdentatus are capable of feeding on various jellyfish species including venomous stingers; however, the range of jellyfish species capable of supporting the growth and survival of phyllosomas is unknown. Seventeen (12 for the first and five for the second trials) and 18 (13 for the first and five for the second trials) phyllosomas were fed exclusively on the jellyfish Aurelia aurita and Chrysaora pacifica, respectively. Aurelia aurita‐fed phyllosomas metamorphosed into the nisto stage (postbenthic larvae) ~54 days after hatching, whereas C. pacifica‐fed phyllosomas did not. Major nutritional compositions such as amino acids, fatty acids and minerals were compared between the two jellyfish species. The proportion of each major nutritional component was not significantly different between the two jellyfish species, suggesting that C. pacifica was not nutritionally inferior to A. aurita. Therefore, the observation that the C. pacifica‐fed phyllosomas did not metamorphose into the nisto stage was not because of major nutritional compositions but due to other factors such as the lack or excess of other minor nutrients, or the species‐specific texture of the jellyfish. 相似文献
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Maritana Mela Francisco Filipak Neto Flávia Yoshie Yamamoto Ronaldo Almeida Sonia Regina Grötzner Dora Fix Ventura Ciro Alberto de Oliveira Ribeiro 《Fish physiology and biochemistry》2014,40(1):245-256
In the present study, we investigated the mercury distribution, mercury bioaccumulation, and oxidative parameters in the Neotropical fish Hoplias malabaricus after trophic exposure. Forty-three individuals were distributed into three groups (two exposed and one control) and trophically exposed to fourteen doses of methylmercury each 5 days, totalizing the doses of 1.05 μg g?1 (M1.05) and 10.5 μg g?1 (M10.5 group). Autometallography technique revealed the presence of mercury in the intestinal epithelia, hepatocytes, and renal tubule cells. Mercury distribution was dose-dependent in the three organs: intestine, liver, and kidney. Reduced glutathione concentration, glutathione peroxidase, catalase, and glutathione S-transferase significantly decreased in the liver of M1.05, but glutathione reductase increased and lipid peroxidation levels were not altered. In the M10.5, most biomarkers were not altered; only catalase activity decreased. Hepatic and muscle mercury bioaccumulation was dose-dependent, but was not influenced by fish sex. The mercury localization and bioaccumulation corroborates some histopathological findings in this fish species (previously verified by Mela et al. in Ecotoxicol Environ Saf 68:426–435, 2007). However, the results of redox biomarkers did not explain histopathological findings previously reported in M10.5. Thus, fish accommodation to the stressor may reestablish antioxidant status at the highest dose, but not avoid cell injury. 相似文献
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Marina HOSOTANI Kiyokazu KAMETANI Nobuhiko OHNO Kohzy HIRAMATSU Takeshi KAWASAKI Yasuhiro HASEGAWA Tomohito IWASAKI Takafumi WATANABE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1764
Typical skeletal muscles are composed of mixed muscle fiber types, which are classified as slow-twitch (type I) and fast-twitch (type II) fibers, whereas pectoralis major muscles (PMs) in broiler chickens are 100% composed of type IIb fast-twitch fibers. Since metabolic properties differ among muscle fiber types, the combination of muscle fiber types is involved in physiological functions and pathological conditions in skeletal muscles. In this study, using serial block-face scanning electron microscopy, we compared three-dimensional (3D) mitochondrial properties in type IIb fibers in broiler PMs and those in type I fibers of broiler gastrocnemius muscles (GMs) heterogeneously composed of slow- and fast-twitch muscle fibers. In type I fibers in the GMs, elongated mitochondria with numerous interconnections to form a substantial network among myofibrils were observed. Along with lipid droplets sandwiched by mitochondria, these features are an adaptation to effective oxidative respiration and constant oxidative damage in slow-twitch muscle fibers. In contrast, type IIb fibers in the PMs showed small and ellipsoid-shaped mitochondria with few interconnections and no lipid droplets, forming a sparse network. The mitochondrial spatial network comprises of active mitochondrial dynamics to reduce mitochondrial damage; therefore, type IIb fibers possess physiologically low capacity to maintain mitochondrial wellness due to static mitochondrial dynamics. Based on 3D mitochondrial properties, we discussed the contrasting physiological functions between type I and IIb fibers and proposed a high contractile power and low stress resistance as unique physiological properties of broiler PMs. 相似文献
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