茶叶中EGCG改善肥胖大鼠肠黏膜屏障损伤的作用研究

为探究EGCG对高脂诱导肥胖大鼠肠黏膜屏障损伤的保护作用,将雄性SD大鼠随机分为正常组、肥胖组、EGCG低剂量及高剂量干预组,每组8只.通过分析大鼠的体重、血清指标、脏器指数及脂肪组织病理切片评估肥胖大鼠脂质聚集与炎症状况及EGCG的干预作用.通过分析肠道病理切片、绒毛长度/隐窝深度值(V/C值)以及血清中脂多糖(LP...     

应用计算机推荐烤烟施肥方案的研究

在辽宁省有代表性的烤烟产区,经过7年的田间试验,得出了在不同肥力水平土壤条件下的烤烟适宜肥料用量范围、肥料效应模型和适宜的施肥方法。应用Viaual Basic高级程序设计语言建立了计算机推荐施肥应用系统。应用系统可根据对不同土壤区域的土壤有效养分含量与施肥量的定量分析对肥料施用数学模型进行数据优化并给出最佳施肥方案,用于烤烟种植推荐施肥与咨询服务,对进一步提高烤烟质量和产量将起到一定的作用。     

我国金针菇工厂化生产的现状、存在问题及对策

分析我国金针菇工厂化产业现状:投资和营收趋稳,产业配套能力明显增强,产业区域分布趋于合理,企业规模化、集团化和合作发展趋势明显。指出目前产业发展中存在的主要问题有技术普及率偏低,生产中还存在一定技术瓶颈问题;专业技术人才紧缺,人才流动频繁;生产过程存在较大资源浪费;产品类型较单一,产品附加值低等。提出打造国际化营商环境,强化科技驱动,加大高层次实战型人才培养,优化产业布局,深挖产业潜能,以及产品品质提升和多元化开发等发展对策。     

桃雄性不育的表现形式及其败育途径

研究了桃［Ｐｒｕｎｕｓ ｐｅｒｃｉｃａ Ｂａｔｓｃｈ（Ｌ．）］２３个品种花粉发生发育和花粉败育过程中细胞形态学上的变化特征。结果表明：桃雄性不育的主要表现形式为空粒花粉和花药空囊及花粉形似发育正常,但生活力极低。导致空粒花粉产生的主要途径：①花粉母细胞减数分裂不正常;②花粉处于单核晚期至二核早、中期绒毡层细胞发生液化、增生现象。空囊产生的主要途径：①四分体至单核早期绒毡层细胞体积发生明显的增大、液化;②绒毡层细胞在四分体至单核早期发生增生;③造胞细胞或花粉母细胞生长发育不良。     

MacCormack算法在长输管道瞬变流动仿真中的应用

为了提高长输管道仿真精度和速率,基于MacCormack格式基本原理和流动方程建立了长输液体管道水力瞬变流动仿真模型。以某管道为例,分别采用该仿真算法和特征线法模拟了阀门关闭和流量增加引起的瞬变流动,并探讨了MacCormack格式中不同边界条件处理方法及Courant常数值对模拟结果的影响。结果表明:MacCormack格式可用于精确仿真长输管道瞬变流动过程,采用特征线法求解预估层边界参数后,其振荡幅度、收敛速度均优于特征线仿真方法;同时,采用特征线法处理MacCormack格式边界条件较线性外插法更易收敛。研究成果可为长输管道瞬变流动仿真提供参考。(图8,表2,参22)     

Bound amino acids in humic acids from arable cropping systems

We investigated the varying concentrations of bound amino acids in humic acids (HA) extracted from soils under both crop rotation and continuous cropping of rye. The experiment was created in 1957. Since then, winter rye had been grown continuously and also the sequence of the 7 yr rotation had been started: potato, spring barley, alfalfa, alfalfa, oil seed rape, winter rye, and winter rye. Soils were fertilized with NPK and manure. Continuous cropping of rye increased total acidity of soils and the contents of carboxylic and phenolic groups in HA. The total amounts of the bound amino acids in HA from soils under crop rotation were higher than from continuous cropping of rye. Fertilization with NPK increased the contents of bound amino acids more than manure. Neutral amino acids dominated in all samples of HA, and basic amino acids had the lowest concentrations. In both types of cultivation, glutamic acids, glycine, alanine, valine, and lysine dominated. The proline contents in HA from continuous rye cropping were higher than in HA from soils under crop rotation. The concentrations of β‐alanine and lysine were higher in HA from crop rotation indicating a higher microbial biomass since these compounds are typical constituents of bacteria cell walls.     

Effects of Temperature,Sonication Time,and Power Settings on Size Distribution and Extractability of Total Wheat Flour Proteins as Determined by Size‐Exclusion High‐Performance Liquid Chromatography

The size‐exclusion (SE) HPLC profile of total protein extract obtained by sonication of flour samples at ambient temperature showed marked instability on reinjection. Instability was related to the presence of flour proteases that were inactivated by thermal treatment of flour samples at 60°C. Extraction of flour protein by sonication was a function of ultrasonic energy (sonication time × power product) delivered to flour sample. As protein solubility increased, the proportions of the earliest eluted SE‐HPLC fractions (F1 and F2) increased. Oversonication of proteins evidenced by a decrease in F1 amount at the benefit of F2 occurred below the sonication energy level needed for total protein extraction. Ultrasonic energy level was adjusted to allow total protein extraction while limiting oversonication. The sonication procedure was applied on 27 flour samples of contrasting dough strength to extract total proteins. Absolute amount of protein extractable by sonication, determined from SE‐HPLC area, was strongly correlated with flour protein content. Very significant and equivalent relationships were found between alveographic W index and absolute amount of either unextractable protein extract or F1 of SE‐HPLC profile from total protein extract.     

正反转旋耕作业的秸秆混埋效果比较

秸秆混埋是增强土壤碳汇的重要技术途经。为分析和比较正、反转旋耕作业的秸秆混埋效果,进行了正、反转旋耕秸秆混埋对比试验。测取旋耕处理后的地貌形态及秸秆在土壤空间中的分布状况,并使用Pro-E造型展示秸秆的空间分布状况。结果表明三维数字化仪配合虚拟造型技术能够直观反映混埋后秸秆在土壤空间的分布状态。2种旋耕处理方式的地表形态及土壤空间内秸秆分布对比分析表明,正转旋耕的秸秆埋覆率及纵向空间分布总体均匀率优于反转旋耕,而反转旋耕的秸秆在沿土壤深度方向的空间分布均匀率、秸秆-土壤混合效果、耕幅内地表平整度等优于正转旋耕。综合分析表明正转旋耕的秸秆混埋质量略有优势,但具体选择混埋模式时还应考虑田间秸秆残留情况。秸秆量较少时反转旋耕较适宜,反之正转旋耕更好。     

气象要素时间分辨率对参考作物蒸散估算的影响

参考作物蒸散(reference evapotranspiration,ET0)的准确估算是农业水资源合理利用的重要环节。为了明确气象要素不同时间分辨率对参考作物蒸散估算的影响,该文基于寿县国家气候观象台2007-2013年观测资料,将1min时间分辨率数据平均值作为真实值,分析了10、20、30、40、60 min、4次/d(02:00、08:00、14:00、20:00)和3次/d(08:00、14:00、20:00)这7种不同时间分辨率对逐日气温、风速、太阳辐射、相对湿度和日、月及年参考作物蒸散(ET0)估算的误差情况。结果表明:ET0和气象要素的误差整体上随时间分辨率降低而增大。4个气象因子中,日平均风速估算受时间分辨率变化的影响最显著,误差最大;其次是太阳辐射。逐日ET0估算在7种时间分辨率的平均绝对相对误差(mean absolute relative error,MAPE)依次为0.53%、1.01%、1.38%、1.72%、2.46%、4.72%和6.14%,表明10至60min时间分辨率的估算效果相较3次/d和4次/d有明显改善。10至40 min的绝对误差超过95%都在-0.20~0.20 mm/d区间内,误差较小且集中度高;太阳辐射时间分辨率变化对ET0估算误差贡献最大,其次是风速,这主要是由于两个要素本身对分辨率较敏感且分别是ET0辐射项和动力项的主要组成因子。时间分辨率的变化对累计后长时间尺度ET0的影响较小,月和年ET0的误差明显小于逐日ET0,月ET0在7种时间分辨率的MAPE值依次为0.13%、0.21%、0.27%、0.40%、0.50%、1.18%和1.48%;各年ET0相对误差(relative error,PE)的绝对值多数均小于0.50%。     

Electron transfer capacity of soil dissolved organic matter and its potential impact on soil respiration

Purpose

Soil dissolved organic matter (DOM) as the labile fraction of soil organic carbon (SOC) is able to facilitate biogeochemical redox reactions effecting soil respiration and carbon sequestration. In this study, we took soil samples from 20 sites differing in land use (forest and agriculture) to investigate the electron transfer capacity of soil DOM and its potential relationship with soil respiration.

Materials and methods

DOM was extracted from 20 soil samples representing different land uses: forest (nos. 1–12) and agriculture (nos. 13–20) in Guangdong Province, China. Chronoamperometry was employed to quantify the electron transfer capacity (ETC) of the DOM, including electron acceptor capacity (EAC) and electron donor capacity (EDC), by applying fixed positive or negative potentials to a working electrode in a conventional three-electrode cell. The reversibility of electron accepting from or donating to DOM was measured by applying switchable potentials to the working electrode in the electrochemical system with the multiple-step potential technique. Carbon dioxide produced by soil respiration was measured with a gas chromatograph.

Results and discussion

Forest soil DOM samples showed higher ETC and electron reversible rate (ERR) than agricultural soil DOM samples, which may be indicative of higher humification rate and microbial activity in forest soils. The average soil respiration of forest soil (nos. 1–12) and agricultural soil (nos. 13–10) was 26.34 and 18.58 mg C g^?1 SOC, respectively. Both EDC and EAC of soil DOM had close relationship with soil respiration (p?<?0.01). The results implied that soil respiration might be accelerated by the electroactive moieties contained in soil DOM, which serve as electron shuttles and facilitate electron transfer reactions in soil respiration and SOC mineralization.

Conclusions

DOM of forest soils showed higher ETC and ERR than DOM of agricultural soils. As soil represents one of the largest reservoirs of organic carbon, soil respiration affects C cycle and subsequently CO₂ concentration in the atmosphere. As one of the important characteristics of soil DOM related to soil respiration, ETC has a significant impact on greenhouse gas emission and soil carbon sequestration but has not been paid attention to.