液相色谱/酶标免疫分析法测定动物组织中已烯雌酚

青岛黄海水产研究所２６６０００ 欧盟及国际社会对在动物性食品严禁使用１，２一二苯乙烯类药物。我国也相应制定了严禁在肉食动物中使用己烯雌酚（ＤＥＳ）等激素的法规。继而我国于 １９９９年制定了《中华人民共和国动物及动物源食品残留监控计划》，加强了对违禁药物的监控力度，规定动物产品中１，２－二苯乙烯类药物的最大残留限量（ＭＲＬ）为不得检出。目前，检测动物产品中ＤＥＳ残留量的方法较多，有紫外分光光度法、液相色谱法、放射免疫法、ＧＣ／ＭＳ法等。各方法各有优缺点。本文根据残留监控过程中既要大批量快速测定，又…     

桑悬浮细胞原生质体培养的研究

采用继代培养三个月后的桑子叶悬浮细胞为材料,进行了原生质体的分离和培养。桑悬浮细胞在纤维素酶、果胶酶、半纤维素酶的混合溶液中,酶解获得产量高、活力强的原生质体。原生质体在K8p(附加6—BA、NAA、2,4—D、LH)液体培养基中,再生细胞经多次分裂,得到肉眼可见的小愈伤组织。再通过增殖继代培养,获得浅黄色、具有明显颗粒结构的愈伤组织,转至各种激素含量的MSB固体培养基中,尚未获得绿苗分化。     

Investigation of Cervical Patency and Uterine Appearance in Domestic Cats by Fluoroscopy and Scintigraphy

The cervical patency of six domestic female cats was monitored under sedation by infusion of contrast medium (Omnipaque) into the cranial vagina during early oestrus, mid‐oestrus, late oestrus and interoestrus or a radiopharmaceutical (^99mTc‐HSA) during mid‐ and interoestrus in a non‐ovulatory oestrous cycle. The transport of the contrast medium or the radiopharmaceutical through the cervix and within the uterine horns was observed under fluoroscopy and with the aid of scintigraphy. In three of the queens, transcervical transport of contrast medium was demonstrated in all stages of oestrus, in one queen during mid‐oestrus, late oestrus and 1 day after oestrus, and in two queens only during late oestrus. The relations between the cervical patency to the contrast medium and the oestrous behaviour, cornification of the vaginal cells and the serum oestradiol‐17β concentration were evaluated, and a relationship was found between the cervical patency and the degree of vaginal cornification. Transcervical transport of the radiopharmaceutical was observed in three queens during mid‐oestrus. When the cervix was open, hysterography under a fluoroscope and hysteroscintigraphy were performed. The fluoroscopic and scintigraphic recordings revealed the patterns of the uterine contractions during oestrus in both ascending and descending directions, and the movement of the uterine contents back and forth between the uterine horns. The hysterograms were classified according to the shape of the uterine horns and the appearance of the endometrial lining. Spiral‐shaped uterine horns with a smooth inner contour were observed in two queens, and a corkscrew appearance with irregular filling defects in the uterine lumen was shown in two queens that had developed subclinical cystic endometrial hyperplasia. These findings demonstrated that fluids or particles deposited in the cranial vagina of the cat can be transported into the uterus during some stages of the oestrous cycle. The fluoroscopic and scintigraphic techniques developed in this study may be further modified to permit more detailed studies of uterine contractile patterns and sperm transport in the feline female reproductive tract. Hysterography proved useful to diagnose uterine disease. The information on cervical patency is of value also for the development of techniques for artificial insemination in this species, and should be studied also in the ovulatory cycle.     

控制sch单养雄蚕品种雌雄蚕孵出比例的催青处理因素研究

研究结果表明 :在RH 6 0 %状态下 ,控制性比的温度和下限时间负相关达到显著水平 ;控制性比的高温处理适于干燥状态下进行 ;处理后雌蚕孵化整齐度明显低于雄蚕孵化整齐度 ,雌雄蚕孵化整齐度均随处理温度的升高而下降 ,尤以雌蚕更为明显。由此提出了在RH 6 0 %状态下sch单养雄蚕品种控制性比的起点温度及温度、时间范围。认为高温催青用下限温度配合 1日收蚁法效果好 ,高温处理以后的胚胎适于在RH 80 %～ 85 %的湿度中保护     

隔膜压滤机在制糖工业中的应用

本文从技术和经济两方面简要介绍了隔膜滤板在制糖行业中的应用，叙述了隔膜板的优点。     

处理桥头跳车的几点认识

本文通过采用常规和采用新材料、新工艺(土工格栅)两种方法对处理桥头跳车现象进行了论述。重点介绍了采用土工格栅处理桥头跳车的具体做法。     

GRC构件在建筑装饰业的应用

本文系统地阐述了GRC构件的产品性能,施工工艺及方法、特点,展望未来建筑业外装饰的发展趋势。     

Reproductive roles predict sexual dimorphism in internal and external morphology of lake whitefish,Coregonus clupeaformis

Abstract – The different reproductive roles of the sexes can predict the direction and magnitude of sexual dimorphism of external and internal morphology. Males should have enlarged structures that enhance the acquisition of mating opportunities, whereas females are predicted to have enlarged organs that are associated with the production of eggs. We tested these predictions in male and female lake whitefish, a species in which both sexes have similar overall body size and shape. After controlling for body size, male lake whitefish had significantly longer jaws and pectoral and pelvic fins, larger hearts, and more muscle than females. Sexual dimorphism in relative muscle mass may be one of the most fundamental morphological differences between males and females. Females had relatively heavier livers than males. Because the liver is important for the breakdown of fats and vitellogenesis, selection should favour an enlarged liver in females for the processing of energy and the production of large numbers of eggs.     

增瘦剂对长×巴猪(F_1)染色体诱变的研究

本文采用外局血淋巴细胞培养及姊妹染色体分染技术,对埋植增瘦剂的长×巴猪(F_1)染色体进行了研究,结果表明,在所统计的细胞中,试验组正常二倍体细胞(2n=3.8)的百分率为71.43％,对照组为80.36％,差异极显著(P<0.01);试验组多倍体细胞数(8.33％)极显著地高于对照组(2.23％,P<0.01);试验组染色体断裂细胞百分率(10.67％)与对照组(8.00％)之间差异不显著(P>0.05);试验组姊妹染色体交换频率(1.88)极显著地高于对照组(0.76,P<0.01)。     

微机综合自动化系统在大型泵站中的应用

水利部“94．8”项目之一“江都抽水机站机组监控关键技术”的实施，在国内首次实现了大型泵站“无人值守，少人值班”的新局面，该系统自动化调节功能，充分利用潮汐河流的特点，实现了泵站的优化调度，提高了运行效率，挖掘了泵站的潜在抽水能力，不仅节约了运行费用，而且还能增灌农田，显著地提高了经济效益和社会效益。