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101.
Hiroaki Kato Shuta Asai Ayako Yamamoto-Katou Hirofumi Yoshioka Noriyuki Doke Kazuhito Kawakita 《Journal of General Plant Pathology》2008,74(1):15-23
During defense responses, plant cells produce nitric oxide (NO), which may control many physiological processes. In a previous
study, we reported that nitrate reductase (NR) is responsible in part for INF1 elicitor-induced NO production in Nicotiana benthamiana, but the possibility remains that other NO-generating system(s) contribute to NO production. In mammalian cells, NO production
is catalyzed by NO synthase (NOS). However, NOS-like enzyme(s) have never been identified in plants, and only the gene for
Arabidopsis thaliana nitric oxide-associated 1 (AtNOA1) has been identified as a putative regulator of NOS activity in plants. In this study, we cloned NbNOA1, a homolog of AtNOA1, from N. benthamiana and investigated its involvement in NO production induced by INF1. The NbNOA1 gene was silenced by a virus-induced gene-silencing (VIGS) technique. NbNOA1-silenced plants had yellowish leaves. Silencing NbNOA1 partially decreased INF1-induced NO production, while overexpressing NbNOA1 did not affect NO production. Silencing NbNOA1 suppressed INF1-induced PR1a gene expression and increased susceptibility to Colletotrichum lagenarium. These results suggest that NbNOA1 is involved in INF1-mediated NO production and is required for defense responses.
The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under accession number AB303300. 相似文献
102.
Fui Fui Ching Yoshizumi Nakagawa Keitaro Kato Osamu Murata Shigeru Miyashita 《Aquaculture Research》2012,43(2):303-310
The effects of delayed first feeding on the survival and growth of tiger grouper, Epinephelus fuscoguttatus (Forsskål 1775), larvae were examined under controlled conditions. The total length, yolk sac volume, oil globule volume, yolk sac absorption time and nutritional transition period (NTP) of the larvae fed at different first times (0, 6, 12, 18 and 24 h after the mouth opening stage; h AMO) were compared. Larval first feed intake was observed at 54 h after hatching (h AH) at 27.5 ± 0.5 °C. The yolk sac was consumed more rapidly with an increase in delayed first feeding and was significantly different among treatments (P<0.05). Larvae first fed at 0 h AMO had the longest yolk sac absorption (72 h AH) and NTP (20 h) times and had the highest survival and growth rates at the end of the experimental period (360 h AH), being significantly higher (P<0.05) than the other treatments. First mortality was observed at 69 h AH, approximately 2 h after point of no return (PNR) occurred. This study suggests that first feeding of tiger grouper larvae should commence at 0 h AMO for enhancement of larval survival and growth. 相似文献
103.
Hirofumi Ido Hirofumi Nagao Hideo Kato Atsushi Miyatake Yasushi Hiramatsu 《Journal of Wood Science》2010,56(5):422-428
Tests of compression perpendicular to the grain were carried out on laminated veneer lumber (LVL) and timber. The species
tested were sugi, radiata pine, karamatsu, akamatsu, and dahurian larch; two sets of sugi specimens were tested, with the
sugi LVL products being manufactured in different plants. The strength properties of the materials for different loading directions
were compared for LVL and timber. At 5% compressive strain in the same materials, the average stress in the tangential direction
of timber was larger than that in the radial direction for all species except for radiata pine, and the average stress in
the edge-wise direction of LVL was larger than that in the flat-wise direction for all species except for radiata pine. When
the stress at 5% strain was compared in the same direction, the average stress of LVL in the edge-wise direction was larger
than that in timber in the tangential direction for all species, but there were no great differences between the average stress
of LVL in the fl at-wise direction and that of timber in the radial direction for all species except for radiation pine. There
was a close relationship between density and stress at 5% strain in LVL, especially in the edge-wise direction. For all results,
radiata pine did not follow the trend of the other species; The large annual ring width of radiata pine was considered to
have affected the results. 相似文献
104.
This study on dried shiitake mushroom [Lentinula edodes (Berk.) Pegler] focuses on the behavior of the aromatic lenthionine compound and its precursor, lentinic acid, during the
drying process. Only a small amount of lenthionine was present after the drying process, regardless of the drying temperature
or time; however, the amount increased during the rehydration process using pH 8.0 buffer. The lenthionine content after incubation
in the buffer was correlated with the lentinic acid content in the dried shiitake mushroom. The lentinic acid content increased
signifi cantly during the drying process at 40°C up to 4 h. The results show that the characteristic smell of dried shiitake
mushroom was weak after drying, and that rehydration in a mild alkaline solution was necessary to increase the smell. The
lentinic acid content of dried shiitake could serve as an indicator of the smell produced by rehydration and would be useful
in screening to select odor-rich strains. Moderate heating likely caused the increase in lentinic acid content, which might
be related to a reaction to heat. 相似文献
105.
Methods to regenerate whole plants from mature leaf explants of Pelargonium rapaceum (L.) L’Hérit were established. To optimize shoot induction, leaf explants were cultured on media containing different types and combinations of plant growth regulators. Growth was initiated within 17–24 days culture, and included callus formation, and root or shoot organogenesis ranging from 20 to 100% regeneration. Shoots were induced only when explants were cultivated on MS medium containing a combination of NAA and kinetin, NAA and BAP, IAA and Kinetin, or IAA and BAP. On media containing NAA and BAP, dark incubation was critical for efficient direct shoot regeneration from explants. Direct shoot formation and the highest number of shoots per explant (17.6) were obtained from leaf explants cultured in the dark for 30 days on MS medium containing 0.1 mg l−1 NAA and 0.1 mg l−1 BAP. Shoots cultured on MS medium containing 0.1 mg l−1 NAA formed tuberous roots with microtubers within 42 days. Healthy regenerated plants were acclimated and transferred to a greenhouse. 相似文献
106.
Masahiko Mori Kento Maki Tsubasa Kawahata Daigo Kawahara Yuta Kato Toru Yoshida Hidetaka Nagasawa Hitoshi Sato Atsushi J. Nagano Paul C. Bethke Kiyoaki Kato 《Breeding Science》2021,71(2):208
Epicotyl length (ECL) of adzuki bean (Vigna angularis) affects the efficiency of mechanized weeding and harvest. The present study investigated the genetic factors controlling ECL. An F2 population derived from a cross between the breeding line ‘Tokei1121’ (T1121, long epicotyls) and the cultivar ‘Erimo167’ (common epicotyls) was phenotyped for ECL and genotyped using simple sequence repeats (SSRs) and single-nucleotide polymorphism (SNP) markers. A molecular linkage map was generated and fifty-two segregating markers, including 27 SSRs and 25 SNPs, were located on seven linkage groups (LGs) at a LOD threshold value of 3.0. Four quantitative trait loci (QTLs) for ECL, with LOD scores of 4.0, 3.4, 4.8 and 6.4, were identified on LGs 2, 4, 7 and 10, respectively; together, these four QTLs accounted for 49.3% of the phenotypic variance. The segregation patterns observed in F5 residual heterozygous lines at qECL10 revealed that a single recessive gene derived from T1121 contributed to the longer ECL phenotype. Using five insertion and deletion markers, this gene was fine mapped to a ~255 kb region near the end of LG10. These findings will facilitate marker-assisted selection for breeding in the adzuki bean and contribute to an understanding of the mechanisms associated with epicotyl elongation. 相似文献
107.
Nitrous oxide evolved from soil covered with plastic mulch film in horticultural field 总被引:1,自引:0,他引:1
Seiichi Nishimura Michio Komada Masako Takebe Seiichiro Yonemura Naoto Kato 《Biology and Fertility of Soils》2012,48(7):787-795
Soil solarization practice, in which soil is covered with plastic mulch film and exposed to high temperature prior to crop cultivation, is expected to be an effective method for reducing weeds and pathogenic microorganisms without using agricultural chemicals. Although the production of nitrous oxide (N2O), a major greenhouse gas, is enhanced in fertilized soil covered with plastic mulch films, its transport route to the atmosphere has not been sufficiently elucidated to date. In this study, we investigated the N2O evolution from plastic-mulch-film-covered agricultural soil. In a horticultural field where ridge soil was covered with a plastic mulch film after fertilization, we observed significant N2O flux from the soil surface of the unfertilized furrow between the ridges, indicating the horizontal diffusion of N2O from the ridge soil covered with the mulch film to the adjacent furrow soil surface. On the other hand, the measurement of the permeance (permeation coefficient) of the plastic mulch film for gaseous N2O by laboratory experiment revealed that N2O gradually permeated the mulch film; the permeance increased exponentially with an increase in ambient temperature, indicating possible N2O emission by permeation through the mulch film under field conditions. In winter, the amount of N2O emission by permeation through the mulch film was estimated to be lower than that emitted from the furrow soil surface, and it was lower than that in summer. On the other hand, it was estimated to be much higher in summer owing to the higher permeance of the film at high temperatures. 相似文献
108.
Tsujimura K Oyama T Katayama Y Muranaka M Bannai H Nemoto M Yamanaka T Kondo T Kato M Matsumura T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(12):1663-1667
A single non-synonymous nucleotide substitution of guanine (G) for adenine (A) at position 2254 in the viral DNA polymerase gene (encoded by open reading frame [ORF] 30) of equine herpesvirus type 1 (EHV-1) has been significantly associated with neuropathogenic potential in strains of this virus. To estimate the prevalence of EHV-1 strains with the neuropathogenic genotype (ORF30 G(2254)) in the Hidaka district--a major horse breeding area in Japan--we analyzed the ORF30 genomic region in cases of EHV-1 infection in this area during the years 2001-2010. Of the 113 cases analyzed, 3 (2.7%) were induced by ORF30 G(2254) strains. This prevalence is lower than those observed in the U.S.A. (10.8-19.4%), Argentina (7.4%), France (24%), and Germany (10.6%). 相似文献
109.
Tsuboi M Etoh H Kato K Nakatugawa H Kato H Maejima Y Matsumoto G Mori H Hosokawa M Miyashita K Tokuda H Suzuki N Maoka T 《Journal of agricultural and food chemistry》2011,59(19):10572-10578
The in vitro reactivity of capsanthin (1) and fucoxanthin (2) with peroxynitrite was investigated, and the reaction products produced by scavenging with peroxynitrite were analyzed. (14'Z)-Nitrocapsanthin (3) and 12-nitrocapsanthin (4) were isolated from the products of the reaction of capsanthin with peroxynitrite. Similarly, (14Z)-15-nitrofucoxanthin (5), (11Z)-11-nitrofucoxanthin (6), and (14Z,9'Z)-15-nitrofucoxanthin (7) were obtained from the reaction of peroxynitrite reaction with fucoxanthin. Capsanthin and fucoxanthin inhibited the nitration of tyrosine by peroxynitrite. Furthermore, nitrocapsanthins (3 and 4) and nitrofucoxanthins (5 and 6) exhibited an inhibitory effect on Epstein-Barr virus early antigen activation in Raji cells and an antiproliferative effect on human pancreatic carcinoma. Moreover, nitrocapsanthins (3 and 4) inhibited carcinogensis of mouse skin tumors initiated by 7,12-dimethylbenz[a]anthracene (DMBN). 相似文献