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101.
Aoshima H Takeda K Okita Y Hossain SJ Koda H Kiso Y 《Journal of agricultural and food chemistry》2006,54(7):2514-2519
Beer induced the response of the ionotropic gamma-aminobutyric acid receptors (GABA(A) receptors) expressed in Xenopus oocytes, indicating the presence of gamma-aminobutyric acid (GABA)-like activity. Furthermore, the pentane extract of the beer, hop (Humulus lupulus L.) oil, and myrcenol potentiated the GABA(A) receptor response elicited by GABA. The GABA(A) receptor responses were also potentiated by the addition of aliphatic esters, most of which are reported to be present in beer flavor. Aliphatic esters showed the tendency to decrease in the potentiation of the GABA(A) receptor response with an increase in their carbon chain length. When myrcenol was injected to mice prior to intraperitoneal administration of pentobarbital, the pentobarbital-induced sleeping time of mice increased additionally. Therefore, the beer contained not only GABA-like activity but also the modulator(s) of the GABA(A) receptor response. 相似文献
102.
Acid deposition in eastern Asia will increase and freshwaters in Japan are likely to become acidified in future. In order to make long-term predictions about freshwater acidification, it is necessary to evaluate acid neutralization mechanisms in Japanese watersheds. Ikeda and Miyanaga (1999) earlier proposed a method of separating acid-neutralization capacity into chemical weathering and cation exchange. By this means, we were able to assess the effect of hydrogeochemical properties on chemical weathering and stream water chemistry for three watersheds in Japan. On the basis of this assessment, acid-neutralization stream water chemistry was predicted using the ILWAS (Integrated Lake-Watershed Acidification Study) model. The main factors determining acidification are the thickness of weatherd profile and chemical weathering rates. The principal results are: (1) for non-acidified watersheds in Japan, acid deposition is neutralized by chemical weathering of primary minerals; (2) freshwaters in Japanese watersheds will not acidify even if acid deposition increases to the extent found in an acidified watershed in the U.S.A. 相似文献
103.
104.
The antineoplastic platinum complexes cisplatin and its analogues are widely used in the chemotherapy of a variety of human malignancies, and are especially active against several types of cancers. Nedaplatin is a second-generation platinum complex with reduced nephrotoxicity. However, their use commonly causes nephrotoxicity due to a lack of tumor tissue selectivity. Several recent studies have provided significant insights into the molecular and histopathological events associated with nedaplatin nephrotoxicity. In this review, we summarize findings concerning the renal histopathology and molecular pathogenesis induced by antineoplastic platinum complexes, with a particular focus on the comparative nephrotoxicity of cisplatin and nedaplatin in rats. 相似文献
105.
Kensuke HIROSE Tatsuya TAKIZAWA Kazuo FUKAWA Tetsuya ITO Masayuki UEDA Yoichi HAYASHI Kazuaki TANAKA 《Animal Science Journal》2011,82(1):46-51
A C?T single nucleotide polymorphism (SNP) on exon 24 of the porcine class 3 phosphoinositide‐3‐kinase (PIK3C3) gene is considered a possible genetic marker for selecting backfat (BF) thickness and carcass fat, although only one study has published results on its effects by performing experiments on a single resource family. We analyzed the association of this PIK3C3 polymorphism with production traits in 739 Duroc pigs. The C allele frequency was 67.9% in our study population. PIK3C3 polymorphism showed significant effects on average daily weight gain (ADG), BF thickness, intermuscular fat content (IMF), and the size of the loin eye muscle area (EMA). The C alleles increased ADG, BF and IMF, and decreased EMA. The predicted differences in traits between the homozygous pigs of the C and T alleles were 40 g/day for DG, 1.2 mm for BF, 0.44% for IMF, and 1.6 cm2 for EMA. Furthermore, the statistical models for estimating the breeding values of each trait had lower Akaike's information criterion values when adding PIK3C3 genotype information. We therefore confirmed that the polymorphism in PIK3C3 (C2604T) has the potential to be a genetic marker for production traits in Duroc pigs. 相似文献
106.
The durability of wood-based panels was evaluated by comparing the bending properties of panels subjected to five accelerated
aging treatments with the bending properties of panels that had experienced 5 years of outdoor exposure in Shizuoka City,
Japan. In each accelerated aging treatment, methylene diphenyl diisocyanate-bonded panels showed higher bending retention
than phenol formaldehyde (PF)-bonded panels. The bending retentions after six repeated cycles of the JIS-B, APA D-1, and ASTM
treatments showed a correspondence of nearly one-to-one in the data for the three different treatments. The Shizuoka City
5-year outdoor exposure test data showed that the bending retentions of all panels decreased with time. In particular, the
bending retentions of PF resin-bonded particleboard and oriented strandboard made from aspen were less than 30% and 10% of
the original values, respectively, after the 5-year exposure period. The deterioration of the bending properties after the
5-year outdoor exposure in Shizuoka City was the same as that for six repetitions of the ASTM treatment. 相似文献
107.
Materials emitting light in the deep ultraviolet region around 200 nanometers are essential in a wide-range of applications, such as information storage technology, environmental protection, and medical treatment. Hexagonal boron nitride (hBN), which was recently found to be a promising deep ultraviolet light emitter, has traditionally been synthesized under high pressure and at high temperature. We successfully synthesized high-purity hBN crystals at atmospheric pressure by using a nickel-molybdenum solvent. The obtained hBN crystals emitted intense 215-nanometer luminescence at room temperature. This study demonstrates an easier way to grow high-quality hBN crystals, through their liquid-phase deposition on a substrate at atmospheric pressure. 相似文献
108.
A number of bioactive marine natural products have been isolated so far, but it is still difficult to disclose their modes of action. In this study, we prepared fluorescently labeled chemical probes from the cytotoxic marine cyclic peptides kapakahines A (1) and F (2) to visualize their localization as the first step of the study of their modes of action. We used fluorescent dyes 3a or 3a/b (a 1:1 mixture of 3a and 3b) whose terminal N-hydroxysuccinimide (NHS) group can react with the free amino groups of kapakahines. The fluorescently labeled kapakahine A (Kap A-5-FL, 5a) stained P388 murine leukemia cells and HeLa human cervical cancer cells, while cells treated with fluorescently labeled kapakahine F (Kap F-5-FL, 6a) only weakly stained them. Further analysis of the confocal images of the stained cells with higher magnification (×100) indicated the localization of Kap A-5-FL (5a) in the cells. In this paper, we report the small-scale preparation and a new delivery method of fluorescent probes, as well as the application of these procedures to cell staining. 相似文献
109.
cDNA cloning of Japanese oyster stress protein homologous to the mammalian 78-kDa glucose regulated protein and its induction by heatshock 总被引:1,自引:0,他引:1
Yoshihiro Yokoyama Hisashi Hashimoto Satoshi Kubota Akira Kuriyama Yoichi Ogura Shoshi Mizuta Reiji Yoshinaka Haruhiko Toyohara 《Fisheries Science》2006,72(2):402-409
ABSTRACT: The 78-kDa glucose regulated protein (GRP78), a member of stress proteins, was cloned from a cDNA library of Japanese oyster Crassostrea gigas . The analysis on Japanese oyster GRP78 clone of approximately 2.6 kb revealed that the entire open reading frame was 1983 bp long and encoded 661 amino acid residues. At the DNA sequence level, the coding region of Japanese oyster GRP78 gene was 72, 62, and 62% identical to those of chicken GRP78, Japanese flounder HSP70, and Japanese flounder HSC71 genes, respectively. Deduced amino acid sequence of Japanese oyster GRP78 was 84, 62, and 62% identical to those of chicken GRP78, Japanese flounder HSP70, and Japanese flounder HSC71, respectively. Japanese oyster GRP78 contained an 18-residue sequence at the N-terminus that exhibits characteristics of a cleavable signal sequence. It also contained an ATPase domain, and a peptide-binding domain in addition to a Lys-Asp-Glu-Leu (KDEL) peptide motif that is involved in determining endoplasmic reticulum localization. Northern blot analysis showed that GRP78 mRNA was induced with heatshock treatment in the oyster tissues. 相似文献
110.
Light-evoked changes in the interphotoreceptor matrix 总被引:2,自引:0,他引:2
The normal function of vertebrate photoreceptor cells depends on multiple interactions and transfer of substances between the photoreceptors and the retinal pigment epithelium (RPE), but the mechanisms of these interactions are poorly understood. Many are thought to be mediated by the interphotoreceptor matrix (IPM), a complex extracellular matrix that surrounds the photoreceptors and lies between them and the RPE. Histochemical, immunocytochemical, and lectin probes for several IPM constituents revealed that components of the IPM in the rat undergo a major shift in distribution or molecular conformation after the transition between light and dark. In the light, various IPM constituents concentrated in bands at the apical and basal regions of the outer segment zone; in the dark, they distributed much more uniformly throughout the zone. The change in IPM distribution was triggered by the light-dark transition; it was not a circadian event, and it was not driven by a systemic factor. The light-evoked change in IPM distribution may facilitate the transfer of substances between the photoreceptors and the RPE. 相似文献