Comparative effects of mu and kappa opiate agonists on the cecocolic motility in the pony.

The electrical and mechanical activity of the large intestine and its response to administration of opiate mu and kappa agonists were assessed from electrodes and inductograph coils chronically implanted on the cecocolic segment in six ponies given a diet of hay and concentrates. Before the drugs were given, migrating complexes propagating from the cecum into the colon occurred at the rate of 1.5 to 16/hour. During this propulsive activity, the cecocolic sphincter opened and closed allowing the outflow of cecal contents and preventing the backflow of colic contents. Each pony was used as its own control and was given fentanyl (0.01 and 0.05 mg/kg of body weight, IV) and U50488H (0.1 and 0.5 mg/kg, IV) at weekly intervals. The mu agonist fentanyl elicited a marked phase of inhibition of the propulsive activity and a closure of the cecocolic sphincter that lasted one to two hours depending on the dose. The kappa agonist U50488H induced an inhibition of the short spiking activity, i.e. of the resting muscle tone. It did not disturb the occurrence of migrating complexes nor that of the openings of the cecocolic sphincter. These kappa compounds may be drugs of choice to alleviate visceral pain in colic stases without inducing delay of transit unlike mu compounds.     

普通小麦抗白粉病配合力及其与过氧化物酶的关系

选用6个亲本组配成双列杂交设计,对普通小麦抗白粉病的配合力及基因效应进行了分析。结果表明,白粉病病情指数属于数量性状遗传,符合加性—显性遗传模型,由加性效应和非加性效应共同控制,且以加性效应为主。低病情指数为部分显性。不同亲本之间一般配合力效应及含有的有利显性基因数存在明显差别。因此,选配杂交组合应在一般配合力高的基础上,注重其特殊配合力的选择。研究还表明,感病品种过氧化物酶活性高。     

葡萄品种需冷量及打破休眠研究

对上海地区葡萄促成栽培的几个主要品种的休眠需冷量进行了调查,发现在供试的5个品种中以里扎马特的需冷量最低,自然条件下仅为447.5h;其次为京亚和巨峰,需冷量均低于1000h;绯红和无核白鸡心需冷量最高,均超过1000h。由此推算,上海地区葡萄结束自然休眠的时间在1月上旬至2月中旬。打破休眠的试验表明以20%石灰氮12月中旬处理效果最佳。     

葡萄二倍体与四倍体品种间杂交胚珠的离体培养

为了克服葡萄二倍体品种与四倍体品种之间的交配障碍,进行了杂交胚珠的离体培养研究。发芽胚珠的取样时期集中于授粉后35～75d,其中多数以二倍体作母本的组合在授粉后55d取样的发芽率最高,以四倍体作母本的组合授粉后70d取样的发芽率最高。基本培养模式为:发育培养基-(剥胚)萌发培养基-生长培养基。用二倍体品种作母本时,所有组合均获得了培养苗,在1号培养基上培养的胚珠发芽率高于2号培养基。反交对培养基的要求有相反趋势,其中2个组合未得到培养苗。授粉后60d,直接剥出杂种胚进行培养,得到了最高发芽率。综合分析初步认为,用二倍体品种作母本比用四倍体品种作母本容易获得杂交后代。     

Pharmacokinetic evaluation of ceftiofur in serum, tissue chamber fluid and bronchial secretions from healthy beef-bred calves.

Ceftiofur is a new broad spectrum cephalosporin marketed for the treatment of acute bovine respiratory disease. In this investigation ceftiofur was administered by intramuscular injection, at 24 h intervals, to healthy beef-bred calves for four days at dosages of 2.2 and 4.4 mg/kg of body weight, with 4 wk intervals between dosing regimens. Serum, tissue chamber fluid (TCF), and bronchial secretion (BS) concentrations of ceftiofur were measured by microbiological assay after the first and fourth dose of each dosing regimen. Peak serum concentrations (Cmax) of 8.8 micrograms/mL and 17.3 micrograms/mL were obtained approximately 2 h (Tmax), the time of mean peak concentration) after single injections of 2.2 mg/kg and 4.4 mg/kg, respectively. The Cmax was increased approximately twofold following multiple doses of 2.2 mg/kg (Cmax = 13.1 micrograms/mL) and 4.4 mg/kg (Cmax = 24.1 micrograms/mL). Ceftiofur accumulated slowly into TCF and peak concentrations were found to be approximately 14% of those observed in serum after the first dose and approximately 24% after multiple dosing. Concentrations of ceftiofur in BS were obtained rapidly with peak concentrations reaching 45% of the serum Cmax after the first dose. After multiple dosing the Cmax for BS was approximately 25% of the serum Cmax. This study found that both the 2.2 mg/kg and 4.4 mg/kg dosing regimens resulted in continuous serum, TCF and BS concentrations of ceftiofur that exceeded the minimal concentration required to inhibit the bacteria most frequently isolated from calves with acute bovine respiratory disease.     

越桔对长白山区酸性土壤的适应性

在长白山区３种类型的酸性土壤上栽培越桔，研究结果表明，｀北空＇、｀美登＇、｀斯卫克＇在暗棕色森林土上生长结果较好，树体营养关系合理；在草甸沼泽土上３个种生长结果均较差，｀北空＇在水湿地潜育土上生长旺盛，但产量低，综合分析认为，矮丛越桔类型品种对我国长白山区酸性土壤条件的适应性较强，半高丛越桔则适应性差，针对长白山不同类型的土壤，以美登最为适宜暗棕色森林土栽培，斯卫克次之；水湿地潜育土可栽培美登品种     

不同氮源对红檬实生苗菌根依赖性的影响

将红檬实生苗培养于改良无土基质中，接种泡囊－丛枝菌根Ｇｌｏｍｕｓｃｉｔｒｏｃｏｌｕｍ，以不接种菌根为对照，并分别施用含氮量相等的３种氮肥，即硝酸按（ＮＯ３－Ｎ）、尿素（ＮＨ２－Ｎ）和硫酸铵（ＮＨ４－Ｎ）。接种菌根１６周后，施不同氮肥的红檬实生苗均有效地感染了菌根，并促进了植株的生长。试验证明，红实生苗施硝态氮（ＮＯ３－Ｎ）使植株的菌根依赖性有明显提高。     

冬季不供热简易产蛋鸡舍的设计与应用研究

通过实验表明:在冬季最低气温-28.3℃的黑龙江省密山市饲养产蛋鸡,只要鸡舍保温良好,适当增加鸡的饲养密度,不用供热,也可保持鸡舍内温度在13℃—15℃的最佳鸡产蛋环境温度;在151天的冬季里实验组鸡,按入舍鸡计算平均每只鸡产蛋103.8个,产蛋率为68.74%,仅比对供热取暖鸡舍的对照组鸡群产蛋率低0.5%,故在寒冷的黑龙江省推广产蛋鸡舍冬季不供热是完全可能的,其经济效益也是显著的。