铺散诸葛菜的组织培养及植株再生

 以铺散诸葛菜的叶柄及叶片为外植体, 研究了不同的再生方式。在6-BA 和NAA 配合使用的MS 培养基上, 叶柄和叶片外植体有高的成芽率, 具有强的器官再生能力。在2,4-D 与6-BA、NAA 结合的培养基上, 叶柄和叶片有较高的愈伤组织诱导率, 在含6-BA 的培养基上也易于分化。叶柄、叶片再生的芽和经愈伤组织分化而得来的芽快速繁殖后, 在生根培养基上形成完整植株。研究表明铺散诸葛菜具有极强的器官分化能力。     

木质包装中黄斑星天牛微波处理初探

为了探索微波处理作为木质包装替代处理方法的可能性,对不同规格和含水量的杨树木块进行微波处理试验,结果表明:频率为2450MHz的微波能够穿透厚度为10cm的杨树木材;当微波功率为900W时,规格为10cm×10cm×10cm和10cm×10cm×2.5cm的新木块中黄斑星天牛(Anoplophora nobolis)幼虫完全死亡所需时间分别为5min和2min;而干木块中则仅需3min和30s.对微波处理引起木材温度变化和水分损失以及天牛幼虫的水分损失进行了初步研究.     

苹果矮化砧木‘辽砧2号’选育

从1135系的助列涅特与M9杂交实生苗中选出优良苹果矮化砧木—辽砧2号(原代号80-1-6),经过22年田间观查和区试鉴定,其矮化性、早果性、丰产性、生根性与M26相近,适应性强于M26,以其为中间砧的富士树栽后6年进入盛果期,平均株产为23.5kg,同龄M26为17.2kg;8年生树冠积为4.07m3,同龄M26为4.58m3,树体存活率较M26高55%,平均每666.7m2产量1000kg以上;与基砧山定子和生产上主栽的富士系、元帅系、国光系等品种亲和性良好,以自根砧和中间砧方式利用均可。     

Relationship of p21WAF1 gene polymorphisms with protein expression in breast carcinomas

AIM: To investigate the relationship between p21^WAF1gene polymorphisms and protein expression in breast carcinoma. METHODS: Polymerase chain reaction single-strand conformation polymorphisms technique (PCR-SSCP) and immunohistochemical assay of S-P immunostaining technique were used to study polymorphisms of p21^WAF1 and protein expression respectively on the specimen of paraffin-embedded tissues in 100 cases of breast carcinomas and 40 benign breast diseases as control. RESULTS: Two p21^WAF1 gene polymorphisms were found in 18% (18/100) of breast carcinomas and 5% (2/40) of control samples. The difference between the two groups was statistically significant (χ²=3.94, P＜0.05). The positive immunohistochemical reaction of p21^WAF1 protein were found in 50% (50/100) of breast carcinomas and 12.5% (5/40) of control samples. The difference between the two groups was statistically significant (χ²=16.84, P＜0.01). The positive immunohistochemical reaction of p21^WAF1 protein were found in 100% (18/18) of breast carcinomas with p21^WAF1 gene polymorphisms and 39% (32/82) of no p21^WAF1 gene polymorphisms. The difference between two groups was statistically significant (χ²=21.95, P＜0.01). The p21^WAF1 gene polymorphisms were correlated with the protein expression in breast carcinomas (r=0.576, P＜0.01). CONCLUSION: p21^WAF1 gene polymorphisms may create the different copies of mRNA and may make relevant protein molecules.     

Recent advances in molecular mechanisms of oncosis

Oncosis is another form of cell death, which is different from apoptosis. The review will discuss the recent advances of oncosis on pathological morphology, nuclear biochemical changes and molecular mechanisms.     

西安荷斯坦奶牛群5个基因座位遗传多态性的PCR-RFLP分析

应用PCR-RFLP方法对西安荷斯坦牛的κ-en、β-lg、β-lg5′侧翼区、CSN1S2、IGFBP-3共5个基因座位进行了多态性分析。结果表明，在西安荷斯坦牛群中，没有发现携带CSN1S2^P等位基因的个体，其多态信息含量为0。κ-en基因座位呈现低度多态(PIC=0．2366)，β-lg、β-lg5′侧翼区、IGFBP-3基因座位的多态信息含量分别为0．3168、0．3689、0．4439，均呈现中度多态。κ-en、β-lg、β-lg5′侧翼区、IGFBP-3、CSNIS2基因座位的杂合度和DNA多态度分别为0．2742、0．3947、0．4879、0．4891、0和0．0255、0．0116、0．0333、0．0112、0。而且，在西安荷斯坦牛群中，κ-en、β-lg、β-lg5′侧翼区、IGFBP-3共4个基因座位均处于Hardy-Weinberg平衡状态，CSN1S2基因座位处于纯合状态。     

国际贸易中的"动物福利"及其影响

“动物福利”这一概念的提出，标志着人们惯有的对待动物甚至对待生命的态度已经转变。“动物福利”不仅要求善待所有的生命，而且也关系到人类的公共道德和环境保护。早在19世纪初，欧洲的一些有识之士就开始了他们捍卫动物的征程。1822年世界上第一部反对虐待动物的法律在英国诞生，许多国家和地区出于对人类健康、环境保护和“动物福利”的考虑，也都相继制定了相关法律来保护动物的基本福利，禁止虐待和伤害动物。近20年来，特别是欧洲发生震惊世界的疯牛病和二恶英污染事件后，发达国家纷纷出台和制订“动物福利”法和饲料添加剂使用法来加强对动物的保护。“动物福利”国家间的差距正在成为农产品国际贸易中的新壁垒形式。     

耕作方式与长期定位施肥对雨养农田冬小麦产量的调控效应

为了明确西北黄土高原半湿润偏旱区耕作方式与长期定位施肥对冬小麦产量的调控效应,以设在半湿润偏旱区连续12年的耕作与肥料长期定位试验为平台,采用裂区设计,以传统耕作和免耕耕作为主处理,不施肥(CK)、单施无机氮肥(N)、单施无机磷肥(P)、单施有机肥(M)、无机氮磷肥配施(NP)、有机无机肥配施(NMP)为副处理,栽培制度为1年春玉米-3年冬小麦轮作,研究耕作及施肥措施对冬小麦产量的影响及其在生产年型间的变化关系。结果表明,不同年型及耕作方式均以有机无机配施冬小麦产量最高,有机肥单施高于化肥单施,磷肥单施高于氮肥单施。耕作方式间表现为传统耕作高于免耕耕作,年型间表现为丰水年>平水年>干旱年,耕作和施肥方式的增产效果以干旱年最好,平水年和丰水年差异不显著。有机无机配施与传统耕作结合优化了冬小麦冠层温度、叶绿素相对含量等生理指标,提高了光合速率、气孔导度及蒸腾速率,因而改善了有效穗、穗粒数和千粒重等产量性状而使冬小麦增产。在550 mm左右降水量的陇东旱塬雨养农业区,无论何种耕作方式及生产年型,长期采用有机无机或无机氮磷肥配施均表现出持续提高冬小麦产量的良好作用。因此,有机无机配施结合传统耕作是提高陇东半湿润偏旱区冬小麦产量的最佳耕作栽培模式。     

Effects of five cryoprotectants on proliferation and differentiation‐related gene expression of frozen‐thawed bovine calf testicular tissue

The cryopreservation of testicular tissue is a potential method for preserving male fertility. However, the effect of cryopreservation on bovine calf testicular tissue is scarce. This study investigated the effect of different cryoprotectants on bovine calf testicular tissue at the molecular level. Testicular tissue from ten immature bovine calves (6 months) was collected after slaughter and cryopreserved in an extender containing different concentrations of the following five cryopreservation solutions (CP): bovine serum albumin (BSA) with 5% dimethyl sulfoxide (DMSO), trehalose with 5% DMSO, DMSO and glycerol and ethylene glycol (EG). After 7‐day cryopreservation, the expression levels of three spermatogonial stem cell (SSC)‐related genes, octamer‐4 (OCT4), KIT ligand (MGF/SCF) and kit oncogene (C‐KIT), were investigated by quantitative PCR (qPCR). The cell viability was highest for the tissues preserved with 30 mg/ml BSA (77.82% ± 1.22) and 40 mg/ml trehalose (74.23% ± 1.16) compared with other groups (p < 0.05), and the level of expression of the three genes was highest with 30 mg/ml BSA (p < 0.05). Compared with other CPs, the 30 mg/ml BSA and 40 mg/ml trehalose have the better cryopreserve protection. The 30 mg/ml BSA is the most viable media for the cryopreservation of testicular tissue from cattle.